quantitative-analysis-random-migration-cells-using-time-lapse-video

Quantitative Analysis of Random Migration of Cells Using Time-lapse Video Microscopy

Cell migration is a dynamic process, which is important for embryonic development, tissue repair, immune system function, and tumor invasion 1, 2. During ...

LSU School of Medicine

The biggest challenge for cancer research is relapses that occur in patients undergoing chemotherapy and radiotherapy, suggesting that some cells in tumors escape targeted treatment. Key questions are why relapses occur and why current therapies fail to remove all cancer cells. The cancer stem-cell hypothesisis based on the fact that not all cells within a tumor are similar. Other than tumorigenesis and metastasis, cancer stem cells have some properties that are similar to those of normal stem cells, such as self-renewal and differentiation. Accordingly, breast cancer stem cells may arise from mutation of normal mammary stem cells or progenitor cells. Cancer stem cell regulation involves several factors, such as Wnt, Notch, and Hedgehog, mutations of which endow cancer stem cells with the capacity for self-renewal. Moreover, epithelial mesenchymal transition and microRNAs recently have been shown to regulate the "stemness " of cancer cells. Targeting cancer stem cells could prevent relapse and provide new hope for cancer prevention.

Breast cancer stem cells: a new challenge for breast cancer treatment.

Although integrins have been implicated in the progression of breast cancer, the exact mechanism whereby they exert this regulation is clearly not understood. To understand the role of integrins in breast cancer, we examined the expression levels of several integrins in mouse breast cancer cell lines by flow cytometry and the data were validated by Western and RT-PCR analysis. The importance of integrins in cell migration and cell invasion was examined by in vitro assays. Further the effect of integrins on metastasis was investigated by in vivo experimental metastasis assays using mouse models.

Article	Year
Biotechnology journal\| PubMed ID: 17582823	2007
The Journal of thoracic and cardiovascular surgery\| PubMed ID: 18805281	2008
Clinical and applied thrombosis/hemostasis : official journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis\| PubMed ID: 19520680	2009
Breast cancer stem cells: a new challenge for breast cancer treatment. Frontiers in bioscience : a journal and virtual library\| PubMed ID: 21196267	2011
Integrin subunits alpha5 and alpha6 regulate cell cycle by modulating the chk1 and Rb/E2F pathways to affect breast cancer metastasis. Molecular cancer\| PubMed ID: 21752283	2011

Prachi Jain

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