repetitive-blood-sampling-from-the-subclavian-vein-of-conscious-rat

Repetitive Blood Sampling from the Subclavian Vein of Conscious Rat

Rats are widely used in pharmacokinetics (PK) and toxicokinetic (TK) studies that need to collect a certain amount of blood at specific time points to ...

Guangdong Engineering Research Center for Innovative Drug Evaluation and Research

Naoxintong Capsule (NXTC), a standardized herbal medicine, has been widely applied in treating cardiovascular and cerebrovascular diseases with remarkable efficacy. However, the efficacy contributing components of NXTC are unclear, and the in vivo absorption and metabolism processes of NXTC remain largely obscured. In this study, using beagle dog as model species, we have identified and tentatively characterized 25 prototype and 15 catabolites of NXTC in beagle dog plasma by ultra-fast liquid chromatography/quadrupole-time-of-flight tandem mass spectrometry (UFLC-Q-TOF-MS/MS). We have proposed the in vivo bio-transformation pathways of these absorbed constituents. In addition, for six crucial components, we have developed a quantitative method and conducted plasma pharmacokinetic study of these six components by rapid resolution liquid chromatography tandem triple quadrupole mass spectrometry (RRLC-QQQ-MS/MS). In conclude, our study provided comprehensive insights into the understanding of the plasma absorbed components profiling of NXTC as well as their in vivo transformation behaviors, which would be of great value for identifying efficacy contributing critical components as well as mechanism related investigations of NXTC in the future.

Pharmacokinetics and biotransformation investigation in beagle dog of active compounds from naoxintong capsule.

Forsythin is the main ingredient of  and is widely used in treatment of fever, viral cold, gonorrhea, and ulcers clinically. This study aimed to evaluate the potential genetic toxicity of forsythin and its safety for human use.

Article	Year
Pharmacokinetics and biotransformation investigation in beagle dog of active compounds from naoxintong capsule. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie\| PubMed ID: 33227707	2021
Genetic Toxicology and Safety Pharmacological Evaluation of Forsythin. Evidence-based complementary and alternative medicine : eCAM\| PubMed ID: 34239584	2021

Jianmin Guo

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