mycorrhizal-maps-as-tool-to-explore-colonization-patterns-fungal

Mycorrhizal Maps as a Tool to Explore Colonization Patterns and Fungal Strategies in the Roots of <em>Festuca rubra</em> and <em>Zea mays</em>

Arbuscular mycorrhizal fungi are symbionts in the roots of plants. Their role is to sustain host development and maintain the nutritional equilibrium in ...

University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca

Excessive application of chemical fertilizers and other agrochemicals can cause large imbalances in soils and agricultural ecosystems. In this context, mycorrhizae represent a viable solution to mitigate these negative effects. Arbuscular mycorrhizae are vital symbionts due to the multiple benefits they bring to both crops and the entire agroecosystem. The main purpose of this study was to observe whether differentiated fertilization has an influence on mycorrhizal colonization patterns in corn. Observed frequencies and intensities of colonization varied widely between phenophases and treatments, with 20% variation for frequency and 14% for intensity, which implies the constant development of both partners during the vegetation period. Arbuscules and vesicles were present in all development stages, but the overall mean was lower than 4% for arbuscules and 1% for vesicles in the analyzed root fragments. Intensity was highly correlated with frequency of colonization compared with arbuscules, where the coefficient was 0.54, and vesicles, with a coefficient of 0.16. Both PCA and NMDS provided good graphical solutions, with a high resolution due to explained variance and good spatial position of vectors. The use of mycorrhizal maps permits the full exploration of colonization patterns and fungal strategy, and the assessment of mycorrhizae-free areas. For the untreated variant, the strategy was oriented toward a longitudinal colonization followed by an irregular development of hyphae with multiple non-colonized areas. Treatment acts to stimulate the appearance of mycorrhizal spots, which further develop radially.

Divergence in Corn Mycorrhizal Colonization Patterns Due to Organic Treatment.

Grassland ecosystems occupy significant areas worldwide and represent a reservoir for biodiversity. These areas are characterized by oligotrophic conditions that stimulate mycorrhizal symbiotic partnerships to meet nutritional requirements. In this study, we selected  for its dominance in the studied mountain grassland, based on the fact that grasses more easily accept a symbiotic partner. Quantification of the entire symbiosis process, both the degree of colonization and the presence of a fungal structure, was performed using the root mycorrhizal pattern method. Analysis of data normality indicated colonization frequency as the best parameter for assessing the entire mycorrhizal mechanism, with five equal levels, each of 20%. Most of the root samples showed an intensity of colonization between 0 and 20% and a maximum of arbuscules of about 5%. The colonization degree had an average value of 35%, which indicated a medium permissiveness of roots for mycorrhizal partners. Based on frequency regression models, the intensity of colonization presented high fluctuations at 50% frequency, while the arbuscule development potential was set to a maximum of 5% in mycorrhized areas. Arbuscules were limited due to the unbalanced and unequal root development and their colonizing hyphal networks. The general regression model indicated that only 20% of intra-radicular hyphae have the potential to form arbuscules. The colonization patterns of dominant species in mountain grasslands represent a necessary step for improved understanding of the symbiont strategies that sustain the stability and persistence of these species.

Mycorrhizal Patterns in the Roots of Dominant  in a High-Natural-Value Grassland.

Exploring grassland microbiomes is a challenge in the current context of linking soil microorganism activity with the balance of these ecosystems. Microbiologists are constantly attempting to develop faster and lower-cost methods, and propose new and best-fitted indicators that will provide a more complex data analysis. A different concept was proposed for assessing functional microbiomes by splitting the functional ecological niche into complementary segments. The comparison with the upper and lower limits of the ecological niche provides a clearer image of community alterations due to long-term applied treatments. The method allows the extraction of the most sensitive and stable functional guilds, with the extraction of the most critical dominant-codominant functional groups in every segment of the functional niche. The resulting microbial functional-sociological model is ready to use on community-level physiological profile databases and also can be applied backward for vegetation analysis.

Article	Year
Divergence in Corn Mycorrhizal Colonization Patterns Due to Organic Treatment. Plants (Basel, Switzerland)\| PubMed ID: 34961236	2021
Mycorrhizal Patterns in the Roots of Dominant in a High-Natural-Value Grassland. Plants (Basel, Switzerland)\| PubMed ID: 35009115	2021
Exploration of Soil Functional Microbiomes-A Concept Proposal for Long-Term Fertilized Grasslands. Plants (Basel, Switzerland)\| PubMed ID: 35567254	2022

Larisa Corcoz

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