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Investigating the Alleviating Effects of <em>Bacillus cereus</em> Administration on Colitis through Gut Microbiota Modulation

Gut microbiota dysbiosis is thought to exert a role in the progression of colitis. However, the precise standards for probiotic administration in ...

Anhui University

Anhui Key Laboratory of Modern Biomanufacturing

Grape seed proanthocyanidin extract (GSPE) that prevents and alleviates the degenerative changes associated with aging has been receiving extensive attention. In our present work, the ageing model was induced by injection of 500 mg kgD-galactose daily for a period of eight weeks. The D-galactose-induced ageing mice model was used for evaluating the effect of GSPE on oxidative stress, inflammation levels and gut microbiota composition. D-Galactose induced oxidative damage and inflammation with a significant increase in malondialdehyde contents, myeloperoxidase activities and the levels of TNF-α and IL-1β, as well as a reduction in the activities of glutathione peroxidase and reduced glutathione. Treatment with different doses of GSPE could significantly improve the antioxidant capacity and inflammation levels in the liver and brain, which is accompanied by increased , , , and , as well as decreased  and . In addition, the high-dose GSPE group exhibited greater potential to delay the ageing process than the low-dose group. Our results also showed that GSPE administration could downregulate the NLRP3 inflammasome signaling pathway for inhibiting inflammation levels in the brain tissue. This study provided a novel strategy to target the gut microbiota with regard to the effect of GSPE administration on alleviating aging-induced alterations  the gut microbiota-liver axis and gut microbiota-brain axis.

Alleviation effects of grape seed proanthocyanidin extract on inflammation and oxidative stress in a D-galactose-induced aging mouse model by modulating the gut microbiota.

Sanghuang mushrooms are medicinal fungi widely used in eastern Asia. In this study, the antioxidant and anti-inflammatory activity of a novel extracellular polysaccharopeptide, sanghuang extracellular polysaccharopeptide (SePSP) was investigated. The SePSP was purified from the submerged fermentation broth of a sanghuang mycelium, Sanghuangporus lonicericola strain CBS17, which was isolated from a wild sanghuang fruiting body.

Purification and antioxidant and anti-Inflammatory activity of extracellular polysaccharopeptide from sanghuang mushroom, Sanghuangporus lonicericola.

Dysbiosis leads to continuous progress of inflammatory bowel disease (IBD). However, current therapeutic approaches for IBD have limited efficacy and are associated with various side effects. This study focused on exploring the positive effect of a new  () strain (HMPM18123) in a colitis mouse model and elucidate the underlying molecular mechanisms. The colitis symptoms were alleviated by the  administration as evidenced by decreased body weight loss, colon length shortening, disease activity index score, and histopathological score. The  mitigated intestinal epithelial barrier damage by upregulating tight junction protein expression. Moreover,  exerted anti-inflammatory effects by regulating macrophage polarization and suppressing the TLR4-NF-κB-NLRP3 inflammasome signaling pathways.  also rebalanced the damaged gut microbiota. Thus, the molecular mechanism of alleviating colitis by  treatment involved the regulation of the TLR4-NF-κB-NLRP3 inflammasome signaling pathways in intestinal mucosal barriers by modulating gut microbiota composition.

Probiotic  Alleviates Dextran Sulfate Sodium-Induced Colitis in Mice through Improvement of the Intestinal Barrier Function, Anti-Inflammation, and Gut Microbiota Modulation.

Limitations in compatibility and effectiveness in delivering bioactive compounds often make it prohibitively difficult to apply Pickering emulsions in wound dressing. In this research, we prepared Pickering emulsion composite hydrogels based on carboxymethyl chitosan - sodium alginate (CMCS-SA) nanoparticles (NPs) stabilized Pickering emulsions, poloxamer 407 (PLX), and curcumin (CUR). CMCS-SA NPs were prepared and used to stabilize Pickering emulsion. The stability of Pickering emulsion improved with the increase of the concentration of NPs, and was highly sensitive to ionic strength change. This Pickering emulsion remained stable at various temperatures. After curcumin were introduced into the emulsion, 0.6% CMCS-SA NPs Pickering emulsion showed controlled release of curcumin in vitro. The CMCS-SA-PLX-CUR hydrogels also exhibited smooth surface and dense structure. This composite hydrogels has antibacterial properties against Escherichia coli and Staphylococcus aureus. Moreover, the CMCS-SA-PLX-CUR hydrogels improved wound healing and increased expression of Ki67 and CD31. RT-qPCR results indicated that the mRNA levels of α-SMA and TGF-β1 in the CMCS-SA-PLX-CUR group were downregulated, while the mRNA levels of TGF-β3 increased. The present study suggests that the potentials of CMCS-SA-PLX-CUR hydrogels are promising in protecting bioactive components and wound care management.

Investigation of construction and characterization of carboxymethyl chitosan - sodium alginate nanoparticles to stabilize Pickering emulsion hydrogels for curcumin encapsulation and accelerating wound healing.

Article	Year
Alleviation effects of grape seed proanthocyanidin extract on inflammation and oxidative stress in a D-galactose-induced aging mouse model by modulating the gut microbiota. Food & function\| PubMed ID: 35043135	2022
Purification and antioxidant and anti-Inflammatory activity of extracellular polysaccharopeptide from sanghuang mushroom, Sanghuangporus lonicericola. Journal of the science of food and agriculture\| PubMed ID: 32767366	2021
Probiotic Alleviates Dextran Sulfate Sodium-Induced Colitis in Mice through Improvement of the Intestinal Barrier Function, Anti-Inflammation, and Gut Microbiota Modulation. Journal of agricultural and food chemistry\| PubMed ID: 34677958	2021
Investigation of construction and characterization of carboxymethyl chitosan - sodium alginate nanoparticles to stabilize Pickering emulsion hydrogels for curcumin encapsulation and accelera... International journal of biological macromolecules\| PubMed ID: 35489626	2022

Yifan Xu

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