microvascular-doppler-assisted-microsurgical-denervation-spermatic

Microvascular Doppler Assisted Microsurgical Denervation of the Spermatic Cord in Treating Chronic Orchialgia

Chronic orchialgia is a common disease in department of urology and andrology. The etiology is complex, and the treatment is difficult. In severe cases, ...

Sun Yat-sen University

Early prediction of erectile dysfunction (ED) is critical in the treatment of impotence. Underlying pathogenesis may be the reason for ED without organic causes in young men.

Glycosylated serum protein may improve our ability to predict endothelial and erectile dysfunction in nonorganic patients.

Ligands that are capable of binding to tumor cell surface biomarkers specifically used in the early diagnosis of cancer and targeted drug delivery in cancer chemotherapy have been extensively investigated. Phage display technology has been demonstrated to be a powerful tool in this field. In this study, the non-small cell lung cancer NCI-H1299 and the normal lung small airway epithelial cell lines were used for subtractive screening in vitro with a phage display 12-peptide library. After three rounds of panning, there was an obvious enrichment in the phages specifically binding to the NCI-H1299 cells, and the output/input ratio of phages increased approximately 875-fold (from 0.4x104 to 3.5x106). A group of peptides capable of binding specifically to the NCI-H1299 cells was obtained, and the affinity of these peptides to bind to the targeted cells and tissues was studied. Through cell-based ELISA, immunocytochemical staining, immunohistochemical staining and immunofluorescence, an M13 phage was isolated and identified from the above screenings, and a synthetic peptide, ZT-1 (sequence QQMHLMSYAPGP), corresponding to the sequence of the surface protein of the M13 phage, was demonstrated to be capable of binding to the tumor cell surfaces of NCI-H1299 and A549 cells and biopsy specimens, but not to normal lung tissue samples, other cancer cells, or non-tumor adjacent lung tissues. In conclusion, the peptide ZT-1 may be a potential candidate biomarker ligand that can be used for targeted drug delivery in lung cancer therapy.

Screening and identification of a peptide specifically targeted to NCI-H1299 cells from a phage display peptide library.

&#x4EE5;&#x7EC6;&#x80DE;&#x8868;&#x9762;&#x53D7;&#x4F53;&#x7684;&#x7279;&#x5B9A;&#x7684;&#x80BD;&#x914D;&#x6709;&#x5DF2;&#x5E7F;&#x6CDB;&#x7528;&#x4E8E;&#x80BF;&#x7624;&#x7814;&#x7A76;&#x548C;&#x4E34;&#x5E8A;&#x5E94;&#x7528;&#x3002;&#x566C;&#x83CC;&#x4F53;&#x5C55;&#x793A;&#x6280;&#x672F;&#x662F;&#x5206;&#x79BB;&#x7684;&#x7EC6;&#x80DE;&#x7279;&#x5F02;&#x6027;&#x80BD;&#x914D;&#x4F53;&#x7684;&#x6709;&#x529B;&#x5DE5;&#x5177;&#x3002;&#x8FDB;&#x884C;&#x7B5B;&#x9009;&#xFF0C;&#x5E76;&#x786E;&#x5B9A;&#x4E3A;&#x80BE;&#x7EC6;&#x80DE;&#x764C;&#x7684;&#x65B0;&#x578B;&#x6807;&#x8BB0;&#xFF0C;&#x6211;&#x4EEC;&#x8BC4;&#x4F30;&#x5DF2;&#x7ECF;&#x786E;&#x5B9A;&#x7684;&#x4E00;&#x79CD;&#x80BD;&#x7684;&#x566C;&#x83CC;&#x4F53;&#x663E;&#x793A;&#x6280;&#x672F;&#x3002;

&#x80BE;&#x7EC6;&#x80DE;&#x764C;&#x7684;&#x7279;&#x5B9A;&#x80BD;&#x7684;&#x566C;&#x83CC;&#x4F53;&#x7684;&#x7B5B;&#x9009;&#x548C;&#x9274;&#x5B9A;&#x663E;&#x793A;&#x80BD;&#x5E93;&#x3002;

Specific peptide ligands to cell surface receptors have been extensively used in tumor research and clinical applications. Phage display technology is a powerful tool for the isolation of cell-specific peptide ligands. To screen and identify novel markers for renal cell carcinoma, we evaluated a peptide that had been identified by phage display technology.

Screening and identification of a renal carcinoma specific peptide from a phage display peptide library.

Ligands peptidiques sp&eacute;cifiques aux r&eacute;cepteurs de surface de cellules ont &eacute;t&eacute; largement utilis&eacute;s dans la recherche de la tumeur et des applications cliniques. Technologie d&#39;affichage bact&eacute;riophage est un outil puissant pour l&#39;isolement des ligands peptidiques sp&eacute;cifiques des cellules. &Agrave; l&#39;&eacute;cran et identifier de nouveaux marqueurs pour cancer du rein, nous avons &eacute;valu&eacute; un peptide qui avait &eacute;t&eacute; identifi&eacute; par le phage technologie d&#39;affichage.

D&eacute;pistage et identification d&#39;un peptide sp&eacute;cifique du carcinome r&eacute;nal d&#39;un phage affichent la biblioth&egrave;que de peptide.

Spezifischen Peptid-Liganden zu Handy-Oberfl&auml;che Rezeptoren wurden ausgiebig in Tumorforschung und klinische Anwendungen verwendet. Phage-Display-Technologie ist ein leistungsf&auml;higes Werkzeug f&uuml;r die Isolierung von zellspezifische Peptid-Liganden. Wir evaluieren um Bildschirm und identifizieren neuartiger Marker f&uuml;r Nierenzellkarzinom, ein Peptid, das bestimmt wurde von Phage display-Technologie.

Screening und Identifizierung von renalem Karzinom spezifischen Peptid aus einem Bakterium anzeigen Peptid-Bibliothek.

Ligands del peptide specifico ai recettori di superfici di cella sono stati ampiamente utilizzati nel tumore ricerca e applicazioni cliniche. Tecnologia di visualizzazione dei fagi &egrave; un potente strumento per l&#39;isolamento di ligandi specifici delle cellule del peptide. Per schermo e identificare nuovi marcatori per il carcinoma a cellule renali, abbiamo valutato un peptide che era stato identificato da fagi della tecnologia di visualizzazione.

Lo screening e l&#39;identificazione di un peptide specifico di carcinoma renale da un fago visualizzazione Libreria del peptide.

&#x7D30;&#x80DE;&#x8868;&#x9762;&#x306E;&#x53D7;&#x5BB9;&#x5668;&#x3078;&#x306E;&#x7279;&#x5B9A;&#x306E;&#x30DA;&#x30D7;&#x30C1;&#x30C9;&#x6027;&#x30EA;&#x30AC;&#x30F3;&#x30C9;&#x306F;&#x816B;&#x760D;&#x306E;&#x7814;&#x7A76;&#x304A;&#x3088;&#x3073;&#x81E8;&#x5E8A;&#x30A2;&#x30D7;&#x30EA;&#x30B1;&#x30FC;&#x30B7;&#x30E7;&#x30F3;&#x3067;&#x5E83;&#x304F;&#x4F7F;&#x7528;&#x3055;&#x308C;&#x3066;&#x3044;&#x307E;&#x3059;&#x3002;&#x30D5;&#x30A1;&#x30FC;&#x30B8;&#x30C7;&#x30A3;&#x30B9;&#x30D7;&#x30EC;&#x30A4;&#x7D30;&#x80DE;&#x7279;&#x7570;&#x30DA;&#x30D7;&#x30C1;&#x30C9;&#x6027;&#x30EA;&#x30AC;&#x30F3;&#x30C9;&#x306E;&#x9694;&#x96E2;&#x306E;&#x305F;&#x3081;&#x306E;&#x5F37;&#x529B;&#x306A;&#x30C4;&#x30FC;&#x30EB;&#x3067;&#x3059;&#x3002;&#x753B;&#x9762;&#x3057;&#x3001;&#x814E;&#x7D30;&#x80DE;&#x764C;&#x306E;&#x65B0;&#x898F;&#x30DE;&#x30FC;&#x30AB;&#x30FC;&#x3092;&#x8B58;&#x5225;&#x3059;&#x308B;&#x306B;&#x306F;&#x3001;&#x6211;&#x3005; &#x304C;&#x540C;&#x5B9A;&#x3055;&#x308C;&#x3066;&#x3044;&#x306A;&#x304B;&#x3063;&#x305F;&#x30DA;&#x30D7;&#x30C1;&#x30C9;&#x8A55;&#x4FA1;&#x6280;&#x8853;&#x306B;&#x3088;&#x3063;&#x3066;&#x30D0;&#x30AF;&#x30C6;&#x30EA;&#x30AA;&#x30D5;&#x30A1;&#x30FC;&#x30B8;&#x3092;&#x8868;&#x793A;&#x3002;

&#x30B9;&#x30AF;&#x30EA;&#x30FC;&#x30CB;&#x30F3;&#x30B0;&#x3068;&#x30D5;&#x30A1;&#x30FC;&#x30B8;&#x304B;&#x3089;&#x814E;&#x764C;&#x7279;&#x5B9A;&#x30DA;&#x30D7;&#x30C1;&#x30C9;&#x306E;&#x540C;&#x5B9A;&#x30DA;&#x30D7;&#x30C1;&#x30C9; &#x30E9;&#x30A4;&#x30D6;&#x30E9;&#x30EA;&#x3092;&#x8868;&#x793A;&#x3057;&#x307E;&#x3059;&#x3002;

&#xC138;&#xD3EC; &#xD45C;&#xBA74; &#xC218;&#xC6A9; &#xCCB4;&#xB97C; &#xD2B9;&#xC815; &#xD3A9; &#xD2F0; &#xB4DC; ligands &#xC885;&#xC591; &#xC5F0;&#xAD6C; &#xBC0F; &#xC784;&#xC0C1; &#xC751;&#xC6A9;&#xC5D0; &#xAD11;&#xBC94;&#xC704; &#xD558; &#xAC8C; &#xC0AC;&#xC6A9; &#xB418;&#xC5C8;&#xC2B5;&#xB2C8;&#xB2E4;. &#xC0B4; &#xADE0; &#xC18C; &#xB514;&#xC2A4;&#xD50C;&#xB808;&#xC774; &#xAE30;&#xC220;&#xC740; &#xC140; &#xAD00;&#xB828; &#xD3A9; &#xD2F0; &#xB4DC; ligands&#xC758; &#xACA9;&#xB9AC;&#xB97C; &#xC704;&#xD55C; &#xAC15;&#xB825;&#xD55C; &#xB3C4;&#xAD6C;&#xC785;&#xB2C8;&#xB2E4;. &#xD654;&#xBA74; &#xC2E0;&#xC7A5; &#xC138;&#xD3EC; &#xC554; &#xC885;&#xC5D0; &#xB300; &#xD55C; &#xC0C8;&#xB85C;&#xC6B4; &#xB9C8;&#xCEE4;&#xB97C; &#xC2DD;&#xBCC4; &#xD558; &#xC6B0;&#xB9AC; &#xD3C9;&#xAC00; &#xD3A9;&#xD0C0;&#xC774;&#xB4DC; &#xBC1C;&#xACAC; &#xD588;&#xB2E4; &#xD558;&#xB294; &#xC0B4; &#xADE0; &#xC18C;&#xC5D0; &#xC758;&#xD574; &#xB514;&#xC2A4;&#xD50C;&#xB808;&#xC774; &#xAE30;&#xC220;.

&#xC2EC;&#xC0AC; &#xBC0F; &#xC2E0;&#xC7A5; &#xC554; &#xD2B9;&#xC815; &#xD3A9;&#xD0C0;&#xC774;&#xB4DC;&#xB294; &#xC0B4; &#xADE0; &#xC18C;&#xC5D0;&#xC11C;&#xC758; &#xD3A9;&#xD0C0;&#xC774;&#xB4DC; &#xB77C;&#xC774;&#xBE0C;&#xB7EC;&#xB9AC;&#xB97C; &#xD45C;&#xC2DC;&#xD569;&#xB2C8;&#xB2E4;.

Ligandos de p&eacute;ptidos espec&iacute;ficos para receptores de superficies celular se han utilizado extensivamente en tumor investigaci&oacute;n y aplicaciones cl&iacute;nicas. Tecnolog&iacute;a de la reproducci&oacute;n del fago es una poderosa herramienta para el aislamiento de ligandos de p&eacute;ptido de c&eacute;lulas espec&iacute;ficas. Para pantalla e identificar nuevos marcadores para el carcinoma de c&eacute;lulas renales, evaluamos un p&eacute;ptido que hab&iacute;a sido identificado por el fago Mostrar la tecnolog&iacute;a.

Detecci&oacute;n e identificaci&oacute;n de un p&eacute;ptido espec&iacute;fico de carcinoma renal de un Fago Mostrar biblioteca de p&eacute;ptidos.

Retraction: Screening and identification of a renal carcinoma specific peptide from a phage display peptide library.

Insulin resistance (IR) triggers endothelial dysfunction, which contributes to erectile dysfunction (ED) and cardiovascular disease.

Insulin resistance is an independent determinate of ED in young adult men.

Expression of the non-receptor tyrosine kinase ETK/BMX has been reported in several solid tumors, but the underlying molecular mechanisms and its clinical significance in renal cell carcinoma (RCC) remain to be elucidated.

The expression and role of tyrosine kinase ETK/BMX in renal cell carcinoma.

Sunitinib monotherapy instead of mitotane combination therapy for the treatment of refractory adrenocortical carcinoma.

To explore the laparoscopic technique with the retroperitoneal approach for complex adrenal tumors.

Retroperitoneal Laparoscopic Surgery in the Treatment of Complex Adrenal Tumors.

Previous studies have demonstrated that the transplantation of alginate-poly-ʟ-lysine-alginate (APA)-encapsulated rat Leydig cells (LCs) provides a promising approach for treating testosterone deficiency (TD). Nevertheless, LCs have a limited capacity to proliferate, limiting the efficacy of LC transplantation therapy. Here, we established an efficient differentiation system to obtain functional Leydig-like cells (LLCs) from human stem Leydig cells (hSLCs). Then we injected APA-encapsulated LLCs into the abdominal cavities of castrated mice without an immunosuppressor. The APA-encapsulated cells survived and partially restored testosterone production for 90 days in vivo. More importantly, the transplantation of encapsulated LLCs ameliorated the symptoms of TD, such as fat accumulation, muscle atrophy and adipocyte accumulation in bone marrow. Overall, these results suggest that the transplantation of encapsulated LLCs is a promising new method for testosterone supplementation with potential clinical applications in TD.

Transplantation of encapsulated human Leydig-like cells: A novel option for the treatment of testosterone deficiency.

Intraepithelial neoplasia is a special type of squamous cell carcinoma occurring in the skin epidermis. The incidence of penile intraepithelial neoplasia in Asian males is rare. We report the clinical characteristics and treatment process of a case of penile intraepithelial neoplasia in a Chinese man. We treated the disease of this patient by surgical excision of the penile lesion and scrotal flap plastic surgery. After surgery, the shape of the penis was satisfactory, and there was no adverse effect on erection. The pathological results confirmed the diagnosis of penile intraepithelial neoplasia. The pathological features showed that the lesion tissue was covered with squamous epithelium and that there was severe atypical hyperplasia of the lesion epithelium, disordered arrangement of polarity, and an intact basement membrane. The removal of the lesions of penile intraepithelial neoplasia through a wider surgical resection range, combined with the stretchability of a scrotal flap, can achieve a good healing effect of the surgical wound and reduce the possibility of recurrence of penile intraepithelial neoplasia.

Case Report: Treatment of Penile Intraepithelial Neoplasia in an Asian Male With Reconstructive Plastic Surgery.

The elderly males undergo degenerative fertility and testicular endocrine function that jeopardize the reproductive health and well-being. However, the mechanisms underlying reproductive aging are unclear. Here, we tried to address this by investigating the phenotypes and transcriptomes of seven regions of the male mouse reproductive tract: the testis, efferent ductules, initial segment, caput, corpus and cauda epididymidis, and vas deferens, in adult (3 months) and aged (21 months) mice. Quantitative PCR, immunohistochemistry, immunofluorescent staining, and enzyme-linked immunosorbent assay were performed for the analysis of gene expression in mice, human tissues, and semen samples. Aged male mice showed both systematic and reproductive changes, and remarkable histological changes were detected in the testis and proximal epididymis. Transcriptomes of the male reproductive tract were mapped, and a series of region-specific genes were identified and validated in mouse and/or human tissues, including Protamine 1 (), ADAM metallopeptidase domain 28 (), Ribonuclease A family member 13 (), WAP four-disulfide core domain 13 (), and . Meanwhile, age-related transcriptome changes of different regions of the male reproductive tract were characterized. Notably, increased immune response was functionally related to the male reproductive aging, especially the T cell activation. An immune response-associated factor, phospholipase A2 group IID (), was identified as a potential biomarker for reproductive aging in mice. And the PLA2G2D level in human seminal plasma surged at approximately 35 years of age. Furthermore, we highlighted Protein tyrosine phosphatase receptor type C (), Lymphocyte protein tyrosine kinase (), Microtubule associated protein tau (), and Interferon induced protein with tetratricopeptide repeats 3 () as critical molecules in the aging of initial segment, caput, caput, and cauda epididymidis, respectively. This study provides an RNA-seq resource for the male reproductive system during aging in mice, and is expected to improve our understanding of male reproductive aging and infertility.

Article	Year
Glycosylated serum protein may improve our ability to predict endothelial and erectile dysfunction in nonorganic patients. The journal of sexual medicine\| PubMed ID: 21143415	2011
Screening and identification of a peptide specifically targeted to NCI-H1299 cells from a phage display peptide library. Molecular medicine reports\| PubMed ID: 21475935	2009
Screening and identification of a renal carcinoma specific peptide from a phage display peptide library. Journal of experimental & clinical cancer research : CR\| PubMed ID: 22071019	2011
Retraction: Screening and identification of a renal carcinoma specific peptide from a phage display peptide library. Journal of experimental & clinical cancer research : CR\| PubMed ID: 22414203	2012
Insulin resistance is an independent determinate of ED in young adult men. PloS one\| PubMed ID: 24391852	2013
The expression and role of tyrosine kinase ETK/BMX in renal cell carcinoma. Journal of experimental & clinical cancer research : CR\| PubMed ID: 24606948	2014
Sunitinib monotherapy instead of mitotane combination therapy for the treatment of refractory adrenocortical carcinoma. International journal of urology : official journal of the Japanese Urological Association\| PubMed ID: 26307384	2015
Retroperitoneal Laparoscopic Surgery in the Treatment of Complex Adrenal Tumors. Cancer management and research\| PubMed ID: 32765081	2020
Transplantation of encapsulated human Leydig-like cells: A novel option for the treatment of testosterone deficiency. Molecular and cellular endocrinology\| PubMed ID: 32980418	2021
Case Report: Treatment of Penile Intraepithelial Neoplasia in an Asian Male With Reconstructive Plastic Surgery. Frontiers in surgery\| PubMed ID: 34262931	2021
Anatomical Transcriptome Atlas of the Male Mouse Reproductive System During Aging. Frontiers in cell and developmental biology\| PubMed ID: 35211476	2021

Xiangan Tu

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