preparation-cytoplasmic-nuclear-long-rnas-from-primary-cultured

Preparation of Cytoplasmic and Nuclear Long RNAs from Primary and Cultured Cells

The separation of intracellular components has been a key tool in cellular biology for many years now and has been able to provide useful insight into how ...

University of Miami

University of Miami Miller School of Medicine

Patients with multiple myeloma-bearing translocation t(11;14) have recently been shown to benefit from the apoptosis-inducing drug venetoclax; however, the drug lacks FDA approval in multiple myeloma thus far due to a potential safety signal in the overall patient population. Selinexor is an inhibitor of nuclear export that is FDA-approved for patients with multiple myeloma refractory to multiple lines of therapy. Here, we report that in four patients with multiple myeloma with t(11;14), the concomitant administration of venetoclax and selinexor was safe and associated with disease response. Moreover, the combination was synergistic in t(11;14) multiple myeloma cell lines and caused decreased levels of Cyclin D1 (which is overexpressed due to the CCND1-IGH fusion) when given in combination as compared to single agents. These data suggest that the combination of venetoclax and selinexor is effective and t(11;14) may serve as a therapeutic marker for response and target for future clinical trials.

Alejandro Pardo

Biography

JoVE Journal Publications

Other Publications