novel-methodological-perspectives-in-the-study-of-mosquito-biology

Novel Methodological Perspectives In The Study Of Mosquito Biology

ARTICLES DISCUSSED:
Bui, M., Li, M., Raban, R. R., Liu, N., Akbari, O. S. Embryo microinjection techniques for efficient site-specific mutagenesis in ...

University of Florida

In this review we examine how exploiting the Wolbachia-mosquito relationship has become an increasingly popular strategy for controlling arbovirus transmission. Field deployments of Wolbachia-infected mosquitoes have led to significant decreases in dengue virus incidence via high levels of mosquito population suppression and replacement, emphasizing the success of Wolbachia approaches. Here, we examine how improved knowledge of Wolbachia-host interactions has provided key insight into the mechanisms of the essential phenotypes of pathogen blocking and cytoplasmic incompatibility. And we discuss recent studies demonstrating that extrinsic factors, such as ambient temperature, can modulate Wolbachia density and maternal transmission. Finally, we assess the prospects of using Wolbachia to control other vectors and agricultural pest species.

Wolbachia as translational science: controlling mosquito-borne pathogens.

Florida lies within a subtropical region where the climate allows diverse mosquito species including invasive species to thrive year-round. As of 2021, there are currently 66 state-approved Florida Mosquito Control Districts, which are major stakeholders for Florida public universities engaged in mosquito research. Florida is one of the few states with extensive organized mosquito control programs. The Florida State Government and Florida Mosquito Control Districts have long histories of collaboration with research institutions. During fall 2020, we carried out a survey to collect baseline data on the current control priorities from Florida Mosquito Control Districts relating to (1) priority control species, (2) common adult and larval control methods, and (3) major research questions to address that will improve their control and surveillance programs. The survey data showed that a total of 17 distinct mosquito species were considered to be priority control targets, with many of these species being understudied. The most common control approaches included truck-mounted ultra-low-volume adulticiding and biopesticide-based larviciding. The districts held interest in diverse research questions, with many prioritizing studies on basic science questions to help develop evidence-based control strategies. Our data highlight the fact that mosquito control approaches and priorities differ greatly between districts and provide an important point of comparison for other regions investing in mosquito control, particularly those with similar ecological settings, and great diversity of potential mosquito vectors, such as in Florida. Our findings highlight a need for greater alignment of research priorities between mosquito control and mosquito research. In particular, we note a need to prioritize filling knowledge gaps relating to understudied mosquito species that have been implicated in arbovirus transmission.

Mosquito Control Priorities in Florida-Survey Results from Florida Mosquito Control Districts.

Mosquito and arbovirus surveillance is essential to the protection of public health. A majority of surveys are undertaken at ground level. However, mosquitoes shelter, breed, and quest for hosts across vertical strata, thus limiting our ability to fully describe mosquito and arboviral communities. To elucidate patterns of mosquito vertical stratification, canopy traps were constructed to sample mosquitoes at heights of 1.5, 5.0, and 8.7 m across three different landscape types in a Florida coastal conservation area. We assessed trapping efforts using individual-based rarefaction and extrapolation. The effects of height, landscape, site location, and sampling date on mosquito community composition were parsed out using permutational ANOVA on a Hellinger-transformed Bray-Curtis dissimilarity abundance matrix. Lastly, a generalized linear mixed effects model (GLMM) was used to explore species-specific vertical patterns. We observed differences in sampling effort and community composition structure across various heights and landscapes. Our GLMM revealed significant effects of trap height for , , , and  but not for , the ultra-dominant species present in this area. Together these data provide evidence that height and landscape significantly affect mosquito community structures and highlight a need to develop sampling regimes to target specific vector and nuisance species at their preferred height and across different landscape types.

Mosquito Communities Vary across Landscape and Vertical Strata in Indian River County, Florida.

Arthropods, including mosquitoes, sand flies, tsetse flies, and ticks are vectors of many bacterial, parasitic, and viral pathogens that cause serious disease in humans and animals. Their microbiota, that is, all microorganisms that dwell within their tissues, can impact vector immunity and susceptibility to pathogen infection. Historically, host-pathogen-microbiota interactions have not been well described, with little known about mechanism. In this review, we highlight recent advances in understanding how individual microorganisms and microbial communities interact with vectors and human pathogens, the mechanisms they utilize to achieve these effects, and the potential for exploiting these interactions to control pathogen transmission. These studies fill important knowledge gaps and further our understanding of the roles that the vector microbiota plays in pathogen transmission.

Article	Year
Wolbachia as translational science: controlling mosquito-borne pathogens. Trends in parasitology\| PubMed ID: 34303627	2021
Mosquito Control Priorities in Florida-Survey Results from Florida Mosquito Control Districts. Pathogens (Basel, Switzerland)\| PubMed ID: 34451411	2021
Mosquito Communities Vary across Landscape and Vertical Strata in Indian River County, Florida. Pathogens (Basel, Switzerland)\| PubMed ID: 34959530	2021
Vector microbiota and immunity: modulating arthropod susceptibility to vertebrate pathogens. Current opinion in insect science\| PubMed ID: 35065286	2022

Eric P. Caragata

Biography

JoVE Journal Publications

Other Publications