establishing-a-diaphyseal-femur-fracture-model-in-mice

Establishing a Diaphyseal Femur Fracture Model in Mice

Bones have a significant regenerative capacity. However, fracture healing is a complex process, and depending on the severity of the lesions and the age ...

Federal University of Rio de Janeiro

This study evaluated the effectiveness of treating pseudarthrosis in rats by using bone marrow cell suspensions or cultures of bone marrow mesenchymal stromal cells

Heterologous mesenchymal stem cells successfully treat femoral pseudarthrosis in rats.

Bone marrow stromal cells (BMSCs) are a valuable resource for skeletal regenerative medicine because of their osteogenic potential. In spite of the very general term "stem cell," this population of cells is far from homogeneous, and different BMSCs clones have greatly different phenotypic properties and, therefore, potentially different therapeutic potential. Adherence to a culture flask surface is a primary defining characteristic of BMSCs. We hypothesized that based on the adherence time we could obtain an enriched population of cells with a greater therapeutic potential. We characterized two populations of bone marrow-derived cells, those that adhered by three days (R-cells) and those that did not adhere by three days but did by six days (L-cells). Clones derived from L-cells could be induced into adipogenic, chondrogenic, and osteogenic differentiation in vitro. L-cells appeared to have greater proliferative capacity, as manifested by larger colony diameter and clones with higher CD146 expression. Only clones from L-cells developed bone marrow stroma in vivo. We conclude that the use of late adherence of BMSCs is one parameter that can be used to enrich for cells that will constitute a superior final product for cell therapy in orthopedics.

Late adherent human bone marrow stromal cells form bone and restore the hematopoietic microenvironment in vivo.

Bone marrow stromal cells (BMSCs, also known as bone marrow mesenchymal stem cells) are a plastic-adherent heterogeneous cell population that contain inherent skeletal progenitors and a subset of multipotential skeletal stem cells (SSCs). Application of BMSCs in therapeutic protocols implies its isolation and expansion under good manufacturing practices (GMP). Here we describe the procedures we have found to successfully generate practical BMSCs numbers, with preserved biological potency.

Article	Year
Heterologous mesenchymal stem cells successfully treat femoral pseudarthrosis in rats. Journal of translational medicine\| PubMed ID: 22429995	2012
Late adherent human bone marrow stromal cells form bone and restore the hematopoietic microenvironment in vivo. BioMed research international\| PubMed ID: 23710460	2013
The Isolation and Manufacture of GMP-Grade Bone Marrow Stromal Cells from Bone Specimens. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 33534112	2021

Danielle Cabral Bonfim

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