experimental-entomology-in-the-age-of-video

Experimental Entomology in the Age of Video

ARTICLES DISCUSSED: 
Smodi&#353; &#352;kerl, M. I. Histology basics and cell death detection in honeybee tissue. Journal of Visualized Experiments. (185), ...

CUNY School of Medicine

City University of New York

Chronic pain is often linked to comorbidities such as anxiety and cognitive dysfunction, alterations that are reflected in brain plasticity in regions such as the prefrontal cortex and the limbic area. Despite the growing interest in pain-related cognitive deficits, little is known about the relationship between the emotional valence of the stimulus and the salience of its memory following painful injuries. We used the tibia fracture model of chronic pain in mice to determine whether pleasant and unpleasant odor location memories differ in their salience seven weeks following the onset of the painful injury. Our results indicate that injured mice show a bias toward recalling unpleasant memories, thereby propagating the vicious cycle of chronic pain and negative affect. Next, we linked these behavioral differences to mechanisms of molecular plasticity by measuring the levels of global methylation and hydroxymethylation in the olfactory bulb. Compared to controls, global methylation levels were shown to be increased, while hydroxymethylation levels were decreased in the olfactory bulb of injured mice, indicative of overall changes in DNA regulation machinery and the subsequent alterations in sensory systems.

Differential olfactory bulb methylation and hydroxymethylation are linked to odor location memory bias in injured mice.

Animal coloration has been rigorously studied and has provided morphological implications for fitness with influences over social behavior, predator-prey interactions, and sexual selection. In vertebrates, its study has developed our understanding across diverse fields ranging from behavior to molecular biology. In the search for underlying molecular mechanisms, many have taken advantage of pedigree-based and genome-wide association screens to reveal the genetic architecture responsible for pattern variation that occurs in early development. However, genetic differences do not provide a full picture of the dynamic changes in coloration that are most prevalent across vertebrates at the molecular level. Changes in coloration that occur in adulthood via phenotypic plasticity rely on various social, visual, and dietary cues independent of genetic variation. Here, I will review the contributions of pigment cell biology to animal color changes and recent studies describing their molecular underpinnings and function. In this regard, conserved epigenetic processes such as DNA methylation play a role in lending plasticity to gene regulation as it relates to chromatophore function. Lastly, I will present African cichlids as emerging models for the study of pigmentation and molecular plasticity for animal color changes. I posit that these processes, in a dialog with environmental stimuli, are important regulators of variation and the selective advantages that accompany a change in coloration for vertebrate animals.

Article	Year
Differential olfactory bulb methylation and hydroxymethylation are linked to odor location memory bias in injured mice. Molecular pain\| PubMed ID: 31407613	2019
Molecular Plasticity in Animal Pigmentation: Emerging Processes Underlying Color Changes. Integrative and comparative biology\| PubMed ID: 33104199	2020

Sebastian Alvarado

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