prospective-randomized-controlled-study-human-umbilical-cord

Prospective, Randomized, and Controlled Study of a Human Umbilical Cord Mesenchymal Stem Cell Injection for Treating Diabetic Foot Ulcers

With the development of society and the economy, the incidence of diabetic foot ulcers continues to increase. Currently, conventional debridement with ...

Affiliated Hospital of Xuzhou Medical University

To compare and analyze the clinical effects of arthroscopic therapy and drug therapy in treating anterior cruciate ligament (ACL) rupture with secondary osteoarthritis (OA).

The curative efficacy of arthroscopic therapy in treating anterior cruciate ligament rupture with secondary osteoarthritis.

New collagen sponge scaffold (NCSS) combined with adipose-derived stem cells (ADSCs) in the repair of full-thickness skin wound in nude mice was investigated. Human ADSCs were extracted via enzyme digestion; NCSS materials were prepared using modified method; the tissue-engineered skin substitute was constructed using ADSCs combined with NCSS. Two 10 mm full-thickness skin wounds were designed on the back of 24 female nude mice, respectively. Mice were divided into 4 groups in the experiment: ADSCs-NCSS (group A), simple NCSS (group B), simple ADSCs (group C) and blank control (group D). The wound healing rates were observed at 3, 7, 10 and 14 days after operation, and specimens were taken at 1 and 2 weeks for histological detection and immunohistochemical cluster of differentiation 31 (CD31) vascular density detection, respectively. At 3 and 7 days after construction of new tissue-engineered skin substitute, the infiltration of ADSCs could be seen within NCSS. The wound healing rates at 7, 10 and 14 days after operation in group A were (77.13±1.25%), (89.90±1.08%) and (96.08±0.6%), respectively, which were significantly higher than those in groups B-D; the differences were statistically significant (p<0.05). The detection of regenerated wound tissue thickness at 1 and 2 weeks after operation and CD31 vascular density at 1 week after operation showed that the vascular density in the wound in group A was significantly higher than those in other groups; the differences were statistically significant (p<0.05). After the transplantation of tissue-engineered skin constructed by human ADSCs combined with NCSS, the quality of wound healing in nude mice can be significantly improved, and the wound repair can be promoted.

Experimental research on ADSCs-NCSS in wound repair.

Nowadays, the use of cryopreserved fat tissue for soft tissue augmentation is common, except for its unpredictable fat graft absorption, and the toxicity of the cryoprotective agent remains a limitation. In this study, the effects of freezing stored fat tissue without a cryoprotector, and the addition of the stromal vascular fraction (SVF) on the survival of cryopreserved transplants was studied.

Effects of Frozen Stromal Vascular Fraction on the Survival of Cryopreserved Fat Tissue.

Cell-assisted lipotransfer (CAL) promotes the survival of fat grafts with high vascular density and improves skin quality by increasing collagen content. However, no study has quantified the changes on the skin surface, and rigorous methodological evaluations are still lacking.

Autologous fat graft assisted by stromal vascular fraction improves facial skin quality: A randomized controlled trial.

Wounds are soft tissue injuries, which are difficult to heal and can easily lead to other skin diseases. Bone marrow mesenchymal stem cells (BMSCs) and the secreted exosomes play a key role in skin wound healing. This study aims to clarify the effects and mechanisms of exosomes derived from BMSCs in wound healing. Exosomes were extracted from the supernatant of the BMSCs. The expression of the micro-RNA miR-93-3p was determined by qRT-PCR analysis. HaCaT cells were exposed to hydrogen peroxide (HO) to establish a skin lesion model. MTT, flow cytometry, and transwell assays were conducted to determine cellular functions. The binding relationship between miR-93-3p and apoptotic peptidase activating factor 1 (APAF1) was measured using a dual luciferase reporter gene assay. The results showed that BMSC-derived exosomes or BMSC-exos promoted proliferation and migration and suppressed apoptosis in HaCaT cells damaged by HO. However, the depletion of miR-93-3p in BMSC-exos antagonized the effects of BMSC-exos on HaCaT cells. In addition, APAF1 was identified as a target of miR-93-3p. Overexpression of APAF1 induced the dysfunction of HaCaT cells. Collectively, the results indicate that BMSC-derived exosomes promote skin wound healing via the miR-93-3p/APAF1 axis. This finding may help establish a new therapeutic strategy for skin wound healing.

Exosomal microRNA⁃93⁃3p secreted by bone marrow mesenchymal stem cells downregulates apoptotic peptidase activating factor 1 to promote wound healing.

Hyaluronic acid (HA) is an effective dermal filler for facial rejuvenation. This study aimed to observe the clinical efficacy of HA injection for lip augmentation in Chinese patients.

Efficacy and safety of hyaluronic acid fillers for lip augmentation in a Chinese population.

The combination of platelet-rich plasma with botulinum toxin A in the treatment of hyaluronic acid embolic cutaneous necrosis and alopecia.

Currently, the lack of clinically accurate measurement and evaluation methods for breast asymmetry has considerably limited the use of autologous fat grafting in the correction of breast asymmetry.

Article	Year
The curative efficacy of arthroscopic therapy in treating anterior cruciate ligament rupture with secondary osteoarthritis. European review for medical and pharmacological sciences\| PubMed ID: 26875887	2016
Experimental research on ADSCs-NCSS in wound repair. Experimental and therapeutic medicine\| PubMed ID: 30542393	2018
Effects of Frozen Stromal Vascular Fraction on the Survival of Cryopreserved Fat Tissue. Aesthetic plastic surgery\| PubMed ID: 30843098	2019
Autologous fat graft assisted by stromal vascular fraction improves facial skin quality: A randomized controlled trial. Journal of plastic, reconstructive & aesthetic surgery : JPRAS\| PubMed ID: 32269011	2020
Exosomal microRNA⁃93⁃3p secreted by bone marrow mesenchymal stem cells downregulates apoptotic peptidase activating factor 1 to promote wound healing. Bioengineered\| PubMed ID: 34898374	2022
Efficacy and safety of hyaluronic acid fillers for lip augmentation in a Chinese population. Journal of cosmetic dermatology\| PubMed ID: 35132743	2022
The combination of platelet-rich plasma with botulinum toxin A in the treatment of hyaluronic acid embolic cutaneous necrosis and alopecia. Dermatologic therapy\| PubMed ID: 35279923	2022
Correction of breast asymmetry by autologous fat grafting with the aid of 3D laser-scanning technology. Asian journal of surgery\| PubMed ID: 35753912	2023

Aijun Zhang

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