rat-model-normothermic-ex-situ-perfused-heterotopic-heart

Rat Model of Normothermic Ex-Situ Perfused Heterotopic Heart Transplantation

Heart transplantation is the most effective therapy for end-stage heart failure. Despite the improvements in therapeutic approaches and interventions, the ...

Chonnam National University Graduate School

Chonnam National University Hospital and Medical School

Chonnam National University Hospital

Extracorporeal membrane oxygenation (ECMO) may be a viable salvage therapy in selected patients with septic shock. As ECMO use increases, we studied left ventricular (LV) performance during sepsis with and without ECMO using a pressure-volume (PV) loop in a murine model and aimed to understand LV hemodynamics in septic shock with ECMO. The rats were divided into Group 1 (ECMO applied to healthy rats), Group 2 (ECMO for septic rats), Group 3 (Controls, n = 20) and Group 4 (Sepsis induction only, n = 20). The cardiac parameters include end-diastolic volume (EDV), end-systolic volume (ESV), end-diastolic pressure (EDP), and end-systolic pressure (ESP), ejection fraction (EF), end-systolic elastance (Ees), diastolic time constant (Tau) index, arterial elastance (Ea), pressure-volume area (PVA), stroke work (SW), and potential energy (PE). We compared the changes of parameters in all groups. A total of 74 rats were included in the analyses. After 2 h on ECMO, Group 2 was associated with significant increases in ESP, EDV, ESV, PVA, PE, and SW. The difference ratio of PE and PVA was significantly higher in Group 2 compared to Group 1 (P < 0.01). In conclusion, myocardial oxygen consumption was higher in septic shock with ECMO than in controls.

Combined effects of sepsis and extracorporeal membrane oxygenation on left ventricular performance in a murine model.

Author Correction: Combined effects of sepsis and extracorporeal membrane oxygenation on left ventricular performance in a murine model.

Extracorporeal membrane oxygenation (ECMO) use has remarkably increased in recent years. Although ECMO has become essential for patients with refractory cardiac and respiratory failure, extracorporeal circulation (ECC) is associated with significant complications. Small-animal models of ECC have been developed and widely used to better understand ECC-induced pathophysiology. This review article summarizes the development of small-animal ECC models, including the animal species, circuit configuration, priming, perioperative procedures, cannulation, and future perspectives of small-animal ECMO models.

Article	Year
Combined effects of sepsis and extracorporeal membrane oxygenation on left ventricular performance in a murine model. Scientific reports\| PubMed ID: 36564422	2022
Author Correction: Combined effects of sepsis and extracorporeal membrane oxygenation on left ventricular performance in a murine model. Scientific reports\| PubMed ID: 36658286	2023
Extracorporeal circulation models in small animals: beyond the limits of preclinical research. Acute and critical care\| PubMed ID: 36935529	2023

Mukhammad Kayumov

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