hydrophobic-salt-modified-nafion-for-enzyme-immobilization

Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization

Over the last decade, there has been a wealth of application for immobilized and stabilized enzymes including biocatalysis, biosensors, and biofuel ...

University of Utah 

Levodopa reportedly inhibits insulin action in skeletal muscle. Here we show that C2C12 myotubes produce levodopa and that insulin-stimulated glucose transport is enhanced when endogenous levodopa is depleted. Exogenous levodopa prevented the stimulation of glucose transport by insulin (P < 0.05) and increased cAMP concentrations (P < 0.05). The decrease in insulin-stimulated glucose transport caused by levodopa was attenuated by propranolol (a beta-adrenergic antagonist) and prevented by NSD-1015 (NSD), an inhibitor of DOPA decarboxylase (DDC; converts levodopa to dopamine). Propranolol and NSD both prevented levodopa-related increases in [cAMP]. However, the effects of levodopa were unlikely to be dependent on the conversion of levodopa to catecholamines because we could detect neither DDC in myotubes nor catecholamines in media after incubation of myotubes with levodopa. The data suggest the possibility of novel autocrine beta-adrenergic action in C2C12 myotubes in which levodopa, produced by myotubes, could have hormone-like effects that impinge on glucose metabolism.

Article	Year
Analytical chemistry\| PubMed ID: 12349985	2002
Journal of neuroscience methods\| PubMed ID: 12706842	2003
Biomacromolecules\| PubMed ID: 15244436	2004
Lab on a chip\| PubMed ID: 15672138	2005
Environmental science & technology\| PubMed ID: 16082955	2005
Possibility of autocrine beta-adrenergic signaling in C2C12 myotubes. Experimental biology and medicine (Maywood, N.J.)\| PubMed ID: 16339749	2005
The Analyst\| PubMed ID: 16440091	2006
Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 16508059	2006
Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 16508071	2006
Inorganic chemistry\| PubMed ID: 16933936	2006
Environmental science & technology\| PubMed ID: 16999091	2006
Current opinion in biotechnology\| PubMed ID: 17399977	2007
Chemical Society reviews\| PubMed ID: 18497931	2008
Bioelectrochemistry (Amsterdam, Netherlands)\| PubMed ID: 18760973	2008
Biosensors & bioelectronics\| PubMed ID: 18774285	2008
Journal of the American Chemical Society\| PubMed ID: 18950156	2008
Biosensors & bioelectronics\| PubMed ID: 19022645	2009
Journal of nanoscience and nanotechnology\| PubMed ID: 19435030	2009
Journal of nanoscience and nanotechnology\| PubMed ID: 19437978	2009
Analytical chemistry\| PubMed ID: 19888713	2009
ACS applied materials & interfaces\| PubMed ID: 20353225	2009
Journal of the American Chemical Society\| PubMed ID: 20405828	2010
The Analyst\| PubMed ID: 20419266	2010
Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 20865383	2011
Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 20865385	2011
Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 20865392	2011
ACS applied materials & interfaces\| PubMed ID: 21043456	2010
Physical chemistry chemical physics : PCCP\| PubMed ID: 21069214	2011
Chemical communications (Cambridge, England)\| PubMed ID: 21203617	2011
Analytical and bioanalytical chemistry\| PubMed ID: 21359574	2011
Analytical chemistry\| PubMed ID: 21627304	2011
The Analyst\| PubMed ID: 21776494	2011
Journal of the American Chemical Society\| PubMed ID: 21902236	2011
Enzyme and microbial technology\| PubMed ID: 22113017	2011
Chemical communications (Cambridge, England)\| PubMed ID: 22227738	2012
Annual review of analytical chemistry (Palo Alto, Calif.)\| PubMed ID: 22524222	2012

Shelley D. Minteer

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