development-evaluation-rat-model-full-thickness-cartilage

Development and Evaluation of a Rat Model of Full-Thickness Cartilage Defects

Cartilage defects of the knee joint caused by trauma are a common sports joint injury in the clinic, and these defects result in joint pain, impaired ...

Zhejiang Chinese Medical University

External snapping hip(ESH) is a vague term used to describe palpable or auditory snapping with hip movements with or without pain. The pathogenesis of ESH is related to the specific anatomical structure and friction factor. The clinical symptom is auditory snapping during activities, physical examination, X-ray, magnetic resonance imaging(MRI), dynamic ultrasound and other imaging techniques can be used to diagnose. Conservative medical management includes rest, avoidance of aggravating activities, and antiinflammatory medications. Treatment Patients with mild symptoms can achieve good results by medication, rest and physiotherapy. Surgical treatment for patients with ineffective conservative treatment was performed. All kinds of open surgery method can achieve good clinical curative effect, arthroscopic surgery is gradually been promoted due to small trauma, less complications. Besides, there are some reports that traditional treatments such as massage, acupuncture and acupotomology have achieved good clinical results, which deserve further study and promotion.

[Research progress on clinical diagnosis and treatment for external snapping hip].

This study assessed the surgical outcomes of Lisfranc injuries accompanied by multiple metatarsal fractures. Metatarsal fractures here refers to metatarsal head, neck, and shaft (including shaft fractures accompanied by fractures of the base) fractures, as well as mixed (i.e., segmental fracture) fractures, as seen on imaging studies.

The surgical outcome of Lisfranc injuries accompanied by multiple metatarsal fractures: A multicenter retrospective study.

Stainless steel wires are often used to fix specific types of fractures in orthopaedic surgery using pliers. This article aims to introduce a new technique to twist steel wires by using an electric drill. The steps before twisting the wire are the same as usual. Our technique is as follows. First, tighten the two ends of wire by using pliers, then insert both the ends of wires into the drill and hold in place. Second, set the drill to reverse mode. Third, start turning the drill, then the two wires begin to intertwine and tighten. It is important to stop turning before the wire (the twisted part) begins to bend. Finally, cut the twisted part of wire in place, and bend the wire stump. This technique can achieve a better appearance while saving the strength of surgeons. It has the same clinical effect as the traditional method. This technique provides a new method for surgery and wide clinical application.

Twisting Steel Wires with a Drill: A New Surgical Technique.

Letters about Published Papers.

This study assessed the reliability and validity of the modified Unified Classification System for femur fractures after hip arthroplasty.

The reliability and validity of the modified Unified Classification System for periprosthetic femoral fractures after hip arthroplasty.

To compare the clinical and radiographic results of the supercapsular percutaneously assisted total hip (SuperPATH) approach and the conventional approach in hip arthroplasty.

A Systematic Review and Meta-Analysis of the SuperPATH Approach in Hip Arthroplasty.

Periprosthetic femoral fracture is identified as the third most frequent reason for revision total hip arthroplasty (THA). Treatment of periprosthetic fractures of the femur after THA remains a surgical challenge. In this report, we presented 2 patients with periprosthetic proximal femur fracture variant (a fracture of the greater trochanter with lateral cortical extension) and femoral stem destabilization.

Open reduction and internal fixation with cables for the variant A Periprosthetic fracture: a case report and literature review.

Correction to: Open reduction and internal fixation with cables for the variant A Periprosthetic fracture: a case report and literature review.

The short-term safety and efficacy of stromal vascular fraction (SVF) in treating knee osteoarthritis (KOA) have been extensively studied but the mid-term and long-term prognoses remain unknown.

Mid-term prognosis of the stromal vascular fraction for knee osteoarthritis: a minimum 5-year follow-up study.

Skin aging is a currently irreversible process, affected by increased oxidative stress, activated cellular senescence, and lacked regeneration of the dermal layer. Mesenchymal stem cells (MSCs), such as human umbilical cord-derived MSCs (hucMSCs), have pro-regeneration and anti-aging potencies. To explore whether hucMSCs can be used to treat skin aging, this study employed skin-aging model of nude mice to conduct in vivo assays, including biochemical analysis of superoxide dismutase (SOD) and malondialdehyde (MDA), gross observation, histopathological observation, and immunohistochemical analysis. To clarify how hucMSCs work on skin aging, this study employed skin-aging model of human dermal fibroblasts (HDFs) to conduct in vitro assays by applying conditional medium of hucMSCs (CMM), including wound healing assay, senescence staining, flow cytometric oxidative detection, real time PCR, and western blot analysis. The in vivo data demonstrated that hucMSCs dose-dependently removed wrinkles, smoothed skin texture, and increased dermal thickness and collagen production of aged skin by reversing SOD and MDA levels and up-regulating Col-1 and VEGF expressions, indicating anti-oxidative and pro-regenerative effects against skin aging. The in vitro data revealed that hucMSCs significantly reversed the senescence of HDFs by promoting cell migration, inhibiting ROS production, and restoring the overexpressions of oxidative and senescent markers through paracrine mode of action, and the paracrine mechanism was mediated by the inhibition of autophagy. This study provided novel knowledge regarding the anti-aging efficacy and paracrine mechanism of hucMSCs on skin, making hucMSCs-based therapy a promising regime for skin aging treatment.

Human Umbilical Cord-Derived Mesenchymal Stem Cells Ameliorate Skin Aging of Nude Mice Through Autophagy-Mediated Anti-Senescent Mechanism.

The commonly used technique for treating unstable pelvic fractures with sacroiliac screws and anterior internal fixator (INFIX) is prone to complications, such as injury to the pelvic vasculature and nerves, life-threatening bleeding, lateral femoral cutaneous neuritis, and wound infection. This study investigated the clinical effects of using a modified percutaneous iliosacral screw and INFIX technique for treating unstable pelvic fractures.

Modified percutaneous iliosacral screw and anterior internal fixator technique for treating unstable pelvic fractures: a retrospective study.

 Knee Osteoarthritis (kOA), the most common joint degenerative disorder, lacks effective therapeutics. Placenta-derived mesenchymal stromal cells (PMSCs) are effective in tissue repairing and generation, which have potential in treating kOA. This study aimed to determine the anti-kOA efficacy of PMSCs and to explore its action mode.  Flow cytometry and three-line differentiation were performed for identification of PMSCs. , a rat kOA model established by anterior cruciate ligament transection (ACLT) surgery was used to evaluate the efficacy of PMSCs. Histopathological HE and SO staining with Osteoarthritis Research Society International scoring were conducted, and cartilage expressions of MMP13 and Col2 were measured by immunohistochemistry. Pain behavior parameters by mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL), were measured. , wound healing and cell immunofluorescence assays were conducted to detect the proliferation and migration ability of chondrocytes treated with PMSCs conditioned medium (PMSCs-CM). Quantitative real-time PCR (qRT-PCR) and Western blot (WB) assays were applied to explore the molecular action of PMSCs on chondrocytes.  The results of flow cytometry indicated that the surface markers of PMSCs (CD73 > 95%, CD90 > 95%, and CD34 < 2%) were consistent with the typical mesenchymal stromal cells. The  data showed that PMSCs significantly reversed the kOA progression by protection of cartilage, regulation of anabolic (Col2) and catabolic (MMP13) expressions, and relief of pain symptoms. The  data showed that PMSCs promoted chondrocyte proliferation and migration and significantly restored the IL-1β-induced abnormal gene expressions of Col2, Mmp13, Adamts4, Adamts5 and Sox9 and also restored the abnormal protein expressions of Col2, Mmp13 and Sox9 of chondrocytes. The molecular actions of PMSCs on chondrocytes in nested co-culture way or in conditioned medium way were similar, confirming a paracrine-based mode of action.  This study demonstrated PMSCs' anti-kOA efficacy and its paracrine-based action mode, providing novel knowledge of PMSCs and suggesting it as a promising cell therapy for treatment of kOA.

Intra-articular injection of placental mesenchymal stromal cells ameliorates pain and cartilage anabolism/catabolism in knee osteoarthritis.

Gastrointestinal dysfunction seriously affects the prognosis and quality of life of patients with multiple fractures. However, experimental evidence of this relationship is lacking. Here we describe a newly developed mouse model of postoperative gastrointestinal dysfunction after multiple fractures.

An Innovative Model of ISS-Based Multiple Fractures and Gastrointestinal Dysfunction Related to c-Kit Protein Expression on Interstitial Cells of Cajal.

Article	Year
[Research progress on clinical diagnosis and treatment for external snapping hip]. Zhongguo gu shang = China journal of orthopaedics and traumatology\| PubMed ID: 29890812	2018
The surgical outcome of Lisfranc injuries accompanied by multiple metatarsal fractures: A multicenter retrospective study. Injury\| PubMed ID: 30587333	2019
Twisting Steel Wires with a Drill: A New Surgical Technique. Orthopaedic surgery\| PubMed ID: 30972913	2019
Letters about Published Papers. Journal of orthopaedic surgery (Hong Kong)\| PubMed ID: 31615324	2019
The reliability and validity of the modified Unified Classification System for periprosthetic femoral fractures after hip arthroplasty. Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association\| PubMed ID: 32229162	2021
A Systematic Review and Meta-Analysis of the SuperPATH Approach in Hip Arthroplasty. BioMed research international\| PubMed ID: 34337015	2021
Open reduction and internal fixation with cables for the variant A Periprosthetic fracture: a case report and literature review. Arthroplasty (London, England)\| PubMed ID: 35236421	2020
Correction to: Open reduction and internal fixation with cables for the variant A Periprosthetic fracture: a case report and literature review. Arthroplasty (London, England)\| PubMed ID: 35236429	2020
Mid-term prognosis of the stromal vascular fraction for knee osteoarthritis: a minimum 5-year follow-up study. Stem cell research & therapy\| PubMed ID: 35279201	2022
Human Umbilical Cord-Derived Mesenchymal Stem Cells Ameliorate Skin Aging of Nude Mice Through Autophagy-Mediated Anti-Senescent Mechanism. Stem cell reviews and reports\| PubMed ID: 35864432	2022
Modified percutaneous iliosacral screw and anterior internal fixator technique for treating unstable pelvic fractures: a retrospective study. BMC musculoskeletal disorders\| PubMed ID: 36471331	2022
Intra-articular injection of placental mesenchymal stromal cells ameliorates pain and cartilage anabolism/catabolism in knee osteoarthritis. Frontiers in pharmacology\| PubMed ID: 36523496	2022
An Innovative Model of ISS-Based Multiple Fractures and Gastrointestinal Dysfunction Related to c-Kit Protein Expression on Interstitial Cells of Cajal. Orthopaedic surgery\| PubMed ID: 36919913	2023

Mengqiang Fan

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