induction-lumbar-disc-degeneration-rabbits-through-transabdominal

Induction of Lumbar Disc Degeneration in Rabbits Through a Transabdominal Approach

Lower back pain is an extremely common medical issue in populations worldwide. One of the main contributors to lower back pain is intervertebral disc ...

Xingtai General Hospital of North China Medical Health Group

Orthopaedic Research Institute of Xingtai

Circular RNAs (circRNAs) are an important class of non-coding RNAs partly by acting as microRNA sponges. Growing evidence indicates that air pollution exposure during pregnancy could lead to congenital defects in the offspring. In this study, using circRNAs sequencing, we profiled differentially expressed circRNAs in rat embryos exposed to a high concentration (> 200 μg/m) of fine particulate matter (PM) in utero. Compared with the control embryos whose mothers were reared in clean air, 25 and 55 circRNAs were found to be downregulated and upregulated, respectively, in the air pollution-exposed group. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses of circRNA-coexpressed genes indicated that segmentation, brain development, and system development together with lysine degradation, Rap1 signaling pathway, and adrenergic signaling were deregulated by in utero air pollution exposure. We also identified the central role of three circRNAs, namely circ_015003, circ_030724, and circ_127215 in the circRNA-microRNA interaction network. These data suggested that circRNA deregulation might play a crucial role in the development of air pollution-associated congenital malformations.

Differentially expressed circular RNAs in air pollution-exposed rat embryos.

Exposure to air pollution is known to increase the risks for cardiovascular, pulmonary and metabolic diseases. Growing evidences also indicated that air pollution exposure during pregnancy could negatively impact on early embryonic development and children's health. We performed RNA sequencing to identify deregulated mRNAs in air pollution-exposed rat embryos. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were used to analyse the potential cellular functions of deregulated mRNAs. Our analysis indicated that a total of 1678 mRNAs were differentially expressed on gestation day 9 upon in utero exposure to fine particulate matter of > 200 μg/m, among which 1098 mRNAs were downregulated and 580 mRNAs were upregulated. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses revealed gap junction, cell adhesion, axon guidance and the neurotrophin signalling pathway as key biological processes perturbed by air pollution exposure. Furthermore, reconstruction of the mRNA regulatory network highlighted the central roles of Tbx4, Bmp4, Sox10, Wnt9b, Bmp7 and Foxc2. These data suggested that embryonic mRNA deregulation may underlie the formation of air pollution-associated congenital defects.

Embryonic gene expression altered by maternal exposure to air pollution in rats.

To compare the biomechanical stability of two-level PLIF constructs with unilateral and bilateral pedicle screw fixations.

Article	Year
Differentially expressed circular RNAs in air pollution-exposed rat embryos. Environmental science and pollution research international\| PubMed ID: 31637615	2019
Embryonic gene expression altered by maternal exposure to air pollution in rats. Environmental science and pollution research international\| PubMed ID: 32500497	2020
Biomechanical Characterization of Unilateral and Bilateral Posterior Lumbar Interbody Fusion Constructs. BioMed research international\| PubMed ID: 35996543	2022

Zhanyong Wu

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