extraction-of-diatom-dna-from-water-samples-and-tissues

Extraction of Diatom DNA from Water Samples and Tissues

Diatom testing is an essential auxiliary means in forensic practice to determine whether the corpse drowned in water and to infer the drowning location. ...

dna-extraction-comparison-between-old-fresh-necrophilic-fly

DNA Extraction and Comparison Between Old and Fresh Necrophilic Fly Samples

A total of five samples of Chrysomya megacephala samples - three fresh samples, one sample stored in alcohol for 2 years, and one sample stored in dry ...

erratum-dna-extraction-comparison-between-old-fresh-necrophilic-fly

Erratum: DNA Extraction and Comparison Between Old and Fresh Necrophilic Fly Samples

This corrects the article ...

Hainan Medical University, Hainan Provincial Academician Workstation (tropical forensic medicine)

To construct STR slippage model and study factors involved in this procedure.

[Constructing of STR slippage model by optimizing some factors].

To establish a whole genome amplification testing system based on degenerate oligonucleotide primed-PCR (DOP-PCR) and to explore its reliability and sensitivity.

[Degenerate oligonucleotide primed-PCR technology establishment and its sensitivity test analysis].

Genetic diversity of 21 autosomal STR loci in the Han Chinese population from Hainan Province in South China.

Genetic analysis of 27 Y-STR loci in Han population from Hainan province, southernmost China.

Population analysis of 27 Y-chromosomal STRs in the Li ethnic minority from Hainan province, southernmost China.

In the present study, population data of 19 autosomal STR loci included in the Goldeneye™ DNA ID System 20A in 653 Li individuals was obtained and population genetic relationships among 13 populations were investigated. MDS and phylogenetic analysis suggested that the Hainan Li population kept a close genetic relationship with the Chinese Han populations, especially for Southern Han populations (Guangdong Han, Sichuan Han, and Hunan Han). Our results indicated that the 19 autosomal STRs are highly discriminative and polymorphic in the Hainan Li population suitable for personal forensic identification and paternity testing.

Population data of 19 autosomal STR loci in the Li population from Hainan Province in southernmost China.

The Ong Be language, an important branch of the Tai-Kadai language family, is one of the most distinctive languages on Hainan Island. Ong Be language speakers, who have lived in the Lingao district of Hainan Island for generations, were classified as Han Chinese in the early days of the establishment of the People's Republic of China but have distinct differences from the Han Chinese in language, lifestyle, customs and values and particularly in appearances and features. Currently, Y-chromosomal short tandem repeat (STR) haplotypes have been widely used in genetic applications, such as human forensics, historical investigations and genealogical research. In this study, 487 unrelated male individuals residing in the Lingao district volunteered, and their Y-STR haplotypes were investigated using the Yfiler and Yfiler Plus with 17 and 27 Y-STR loci, respectively. Furthermore, we combined our population data on the Lingao people with existing datasets from Asian nations (East, South and Southeast Asia) to explore the genetic variance and relationships with Han Chinese from different administrative regions in Northern and Southern China and Chinese ethnic minorities officially recognized by the PRC. Population comparisons demonstrated that the Lingao people had distant relationships with Asian nations at the national level and had relatively close genetic and linguistic relationships with Hainan Li and Guizhou Gelao, both of whom belong to the Tai-Kadai language family. The present results increase our understanding of the genetic relationships between the Lingao people and other groups, and further research in genetics and other areas is still needed to trace the origin of the Lingao people.

The evaluation of forensic characteristics and the phylogenetic analysis of the Ong Be language-speaking population based on Y-STR.

The Ong Be language-speaking population (Lingao population) settled in the north-central coast of Hainan Island and has attracted little attention because of its small population size (about five hundred thousand) as well as its relative geographical isolation in linguistics, anthropology, and forensic genetics. The Lingao population selected "Han Chinese" as its ethnic component around the founding period of the PRC. Hence, we used the Goldeneye™ DNA ID System 20A (including 13 CODIS core loci and 6 expanded CODIS loci) to obtain Lingao population genotypes and to enable the publishing of relative forensic parameters; further, this data will allow the evaluation of the Lingao ethnic component from different perspectives. Genetic differences between the Lingao population and Han Chinese populations from north and south administrative divisions of China as well as genetic distinctions among official ethnic groups were also investigated by the principal component analysis (PCA). The phylogenic tree was investigated by the unweighted pair-group method with arithmetic averages (UPGMA). We analysed the genetic polymorphisms of 19 autosomal STR loci in 821 individuals from the Lingao population and observed a total of 269 alleles at 19 autosomal STR loci, with the corresponding allelic frequencies ranging from 0.0006 to 0.5780. The combined power of discrimination (CPD) and combined power of exclusion (CPE) for the 19 autosomal STR loci were 0.99999999999999999999998569 and 0.999999989, respectively. No evidence of deviation from the Hardy-Weinberg equilibrium (HWE) was identified and no linkage disequilibrium (LD) was observed. The results demonstrated that the Goldeneye™ DNA ID System 20A had highly genetic diversities in the 19 STR loci in the Lingao population for forensic applications. In addition, the Lingao population had relatively close genetic relationships with Guangxi Han and Hainan Li populations compared to other populations. However, from a historical and linguistic perspective, "Han Chinese" is probably not an accurate description of the Lingao population. In conclusion, it is neither accurate or appropriate to classify the Ong Be language-speaking population as "Han Chinese" for multiple reasons. The present study can increase our understanding of the genetic relationships between the Lingao population and other Chinese groups. Nonetheless, further genetic studies are still needed to explore the mysteries of the Ong Be language-speaking population.

ArticleTotal : 3	Year
Publication title Cited by 2	2023
Publication title Cited by 3	2024
Publication title	2024

Article	Year
[Constructing of STR slippage model by optimizing some factors]. Fa yi xue za zhi\| PubMed ID: 16524184	2006
[Degenerate oligonucleotide primed-PCR technology establishment and its sensitivity test analysis]. Fa yi xue za zhi\| PubMed ID: 22435337	2012
Genetic diversity of 21 autosomal STR loci in the Han Chinese population from Hainan Province in South China. Forensic science international. Genetics\| PubMed ID: 27510303	2016
Genetic analysis of 27 Y-STR loci in Han population from Hainan province, southernmost China. Forensic science international. Genetics\| PubMed ID: 29276015	2018
Population analysis of 27 Y-chromosomal STRs in the Li ethnic minority from Hainan province, southernmost China. Forensic science international. Genetics\| PubMed ID: 29409735	2018
Population data of 19 autosomal STR loci in the Li population from Hainan Province in southernmost China. International journal of legal medicine\| PubMed ID: 29557504	2019
The evaluation of forensic characteristics and the phylogenetic analysis of the Ong Be language-speaking population based on Y-STR. Forensic science international. Genetics\| PubMed ID: 30279073	2018
The Ong Be language-speaking population in Hainan Island: genetic diversity, phylogenetic characteristics and reflections on ethnicity. Molecular biology reports\| PubMed ID: 31098804	2019

Jian-qiang Deng

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