Robert Glaeser

Molecular Biophysics and Integrative Bio-Imaging Division, Lawrence Berkeley National Laboratory

Affiliated withLawrence Berkeley National Laboratory

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JoVE Journal Publications

ArticleTotal : 1	Year
Publication title Cited by 15	2023

Other Publications

Article	Year
Modeling chemical bonding effects for protein electron crystallography: the transferable fragmental electrostatic potential (TFESP) method. Acta crystallographica. Section A, Foundations of crystallography\| PubMed ID: 11832586	2002
A giant protease with a twist: the TPP II complex from Drosophila studied by electron microscopy. The EMBO journal\| PubMed ID: 12426370	2002
Crystallization of membrane proteins from media composed of connected-bilayer gels. Biopolymers\| PubMed ID: 12539259	2002
Crystal structure of the bromide-bound D85S mutant of bacteriorhodopsin: principles of ion pumping. Biophysical journal\| PubMed ID: 12829500	2003
Specificity of anion binding in the substrate pocket of bacteriorhodopsin. Biochemistry\| PubMed ID: 15109251	2004
Electron microscopy of biological macromolecules: bridging the gap between what physics allows and what we currently can get. Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada\| PubMed ID: 15306063	2004
High-throughput film-densitometry: an efficient approach to generate large data sets. Journal of structural biology\| PubMed ID: 15629654	2005
Particle picking by segmentation: a comparative study with SPIDER-based manual particle picking. Journal of structural biology\| PubMed ID: 16330229	2005
The parallelization of SPIDER on distributed-memory computers using MPI. Journal of structural biology\| PubMed ID: 16859923	2007
Stroboscopic image capture: reducing the dose per frame by a factor of 30 does not prevent beam-induced specimen movement in paraffin. Ultramicroscopy\| PubMed ID: 16905258	2007
SPARX, a new environment for Cryo-EM image processing. Journal of structural biology\| PubMed ID: 16931051	2007
Design of a microfabricated, two-electrode phase-contrast element suitable for electron microscopy. Ultramicroscopy\| PubMed ID: 17079082	2007
Octomeric pyruvate-ferredoxin oxidoreductase from Desulfovibrio vulgaris. Journal of structural biology\| PubMed ID: 17400475	2007
Macromolecular structures without crystals. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 18256181	2008
Restoration of weak phase-contrast images recorded with a high degree of defocus: the "twin image" problem associated with CTF correction. Ultramicroscopy\| PubMed ID: 18508199	2008
Retrospective: radiation damage and its associated "information limitations". Journal of structural biology\| PubMed ID: 18588985	2008
Retrospective on the early development of cryoelectron microscopy of macromolecules and a prospective on opportunities for the future. Journal of structural biology\| PubMed ID: 18606231	2008
Practical factors affecting the performance of a thin-film phase plate for transmission electron microscopy. Ultramicroscopy\| PubMed ID: 19157711	2009
A method for the alignment of heterogeneous macromolecules from electron microscopy. Journal of structural biology\| PubMed ID: 19166941	2009
Experimental characterization and mitigation of specimen charging on thin films with one conducting layer. Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada\| PubMed ID: 19780320	2004
Survey of large protein complexes in D. vulgaris reveals great structural diversity. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 19805340	2009
Precise beam-tilt alignment and collimation are required to minimize the phase error associated with coma in high-resolution cryo-EM. Journal of structural biology\| PubMed ID: 21182964	2011
The surface of evaporated carbon films is an insulating, high-bandgap material. Journal of structural biology\| PubMed ID: 21338687	2011
Reaching the information limit in cryo-EM of biological macromolecules: experimental aspects. Biophysical journal\| PubMed ID: 21575566	2011
Design of a hybrid double-sideband/single-sideband (schlieren) objective aperture suitable for electron microscopy. Ultramicroscopy\| PubMed ID: 22088443	2011
Human tripeptidyl peptidase II: a gentle giant. Structure (London, England : 1993)\| PubMed ID: 22483101	2012
Electron microscopy of biotinylated protein complexes bound to streptavidin monolayer crystals. Journal of structural biology\| PubMed ID: 22584152	2012
Electron microscopy of biological specimens in liquid water. Biophysical journal\| PubMed ID: 22828343	2012
Deprotonation of D96 in bacteriorhodopsin opens the proton uptake pathway. Structure (London, England : 1993)\| PubMed ID: 23394942	2013
Ranking TEM cameras by their response to electron shot noise. Ultramicroscopy\| PubMed ID: 23747527	2013
Minimizing electrostatic charging of an aperture used to produce in-focus phase contrast in the TEM. Ultramicroscopy\| PubMed ID: 23872037	2013
Optimal and fast rotational alignment of volumes with missing data in Fourier space. Journal of structural biology\| PubMed ID: 23994045	2013
Replication and validation of cryo-EM structures. Journal of structural biology\| PubMed ID: 24036314	2013
Invited review article: Methods for imaging weak-phase objects in electron microscopy. The Review of scientific instruments\| PubMed ID: 24289381	2013
Generalization of the Matsumoto-Tonomura approximation for the phase shift within an open aperture. Ultramicroscopy\| PubMed ID: 24333773	2014
Disulfide linkage and structure of highly stable yeast-derived virus-like particles of murine polyomavirus. The Journal of biological chemistry\| PubMed ID: 24567335	2014
Automated particle correspondence and accurate tilt-axis detection in tilted-image pairs. Journal of structural biology\| PubMed ID: 24694675	2014
Factors that Influence the Formation and Stability of Thin, Cryo-EM Specimens. Biophysical journal\| PubMed ID: 26386606	2016
How good can cryo-EM become? Nature methods\| PubMed ID: 26716559	2016
Protein complexes in focus. eLife\| PubMed ID: 27124202	2016
Long shelf-life streptavidin support-films suitable for electron microscopy of biological macromolecules. Journal of structural biology\| PubMed ID: 27320699	2016
Specimen Behavior in the Electron Beam. Methods in enzymology\| PubMed ID: 27572722	2016
Monolayer-crystal streptavidin support films provide an internal standard of cryo-EM image quality. Journal of structural biology\| PubMed ID: 28259651	2017
Multi-pass transmission electron microscopy. Scientific reports\| PubMed ID: 28490730	2017
Opinion: hazards faced by macromolecules when confined to thin aqueous films. Biophysics reports\| PubMed ID: 28781996	2017
Estimating the effect of finite depth of field in single-particle cryo-EM. Ultramicroscopy\| PubMed ID: 28869854	2018
How Cryo-EM Became so Hot. Cell\| PubMed ID: 29195065	2017
PROTEINS, INTERFACES, AND CRYO-EM GRIDS. Current opinion in colloid & interface science\| PubMed ID: 29867291	2018
How Good Can Single-Particle Cryo-EM Become? What Remains Before It Approaches Its Physical Limits? Annual review of biophysics\| PubMed ID: 30786229	2019
Current outcomes when optimizing 'standard' sample preparation for single-particle cryo-EM. Journal of microscopy\| PubMed ID: 31553060	2019
Laser phase plate for transmission electron microscopy. Nature methods\| PubMed ID: 31562475	2019
Microscale Fluid Behavior during Cryo-EM Sample Blotting. Biophysical journal\| PubMed ID: 31952802	2020
Observation of the Relativistic Reversal of the Ponderomotive Potential. Physical review letters\| PubMed ID: 32412292	2020
Spectral DQE of the Volta phase plate. Ultramicroscopy\| PubMed ID: 32739753	2020
Defocus-dependent Thon-ring fading. Ultramicroscopy\| PubMed ID: 33524638	2021
Preparing Better Samples for Cryo-Electron Microscopy: Biochemical Challenges Do Not End with Isolation and Purification. Annual review of biochemistry\| PubMed ID: 33556280	2021
Minimizing Crinkling of Soft Specimens Using Holey Gold Films on Molybdenum Grids for Cryogenic Electron Microscopy. Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada\| PubMed ID: 34085628	2021
High-power near-concentric Fabry-Perot cavity for phase contrast electron microscopy. The Review of scientific instruments\| PubMed ID: 34243315	2021
Simple assay for adsorption of proteins to the air-water interface. Journal of structural biology\| PubMed ID: 34534654	2021
Conquer by cryo-EM without physically dividing. Biochemical Society transactions\| PubMed ID: 34709401	2021
Perspective: Emerging strategies for determining atomic-resolution structures of macromolecular complexes within cells. Journal of structural biology\| PubMed ID: 34915129	2022
Perspective: Biochemical and Physical Constraints Associated With Preparing Thin Specimens for Single-Particle Cryo-EM. Frontiers in molecular biosciences\| PubMed ID: 35573724	2022
Overcoming resolution loss due to thermal magnetic field fluctuations from phase plates in transmission electron microscopy. bioRxiv : the preprint server for biology\| PubMed ID: 36824829	2023
Overcoming resolution loss due to thermal magnetic field fluctuations from phase plates in transmission electron microscopy. Ultramicroscopy\| PubMed ID: 37011498	2023
Challenges in making ideal cryo-EM samples. Current opinion in structural biology\| PubMed ID: 37392555	2023
Overcoming Resolution Loss in Laser Phase Plate Cryo-Electron Microscopy. Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada\| PubMed ID: 37613521	2023