portable-paper-based-immunoassay-combined-with-smartphone-application

Portable Paper-Based Immunoassay Combined with Smartphone Application for Colorimetric and Quantitative Detection of Dengue NS1 Antigen

Dengue virus (DENV) infection, which is transmitted by Aedes mosquitoes, is a major public health concern in tropical and subtropical countries. With an ...

Universitas Islam Indonesia

King Mongkut's University of Technology Thonburi

The diagnosis of dengue infection is still a critical factor determining success in the clinical management and treatment of patients. Here, the development of microfluidic paper-based analytical devices (μPADs) utilizing a sandwich immunoassay on wax patterned paper functionalized with anti-dengue NS1 monoclonal antibodies for point-of-care detection of dengue NS1 (DEN-NS1-PAD) is reported. Various assay conditions, including the length of the channel and diluent, were optimized, and the response detected by the naked eye and digitized images within 20-30 min. The DEN-NS1-PAD was successfully tested in the field for detecting dengue NS1 in buffer, cell culture media, and human serum. The limit of detection (LoD) of the DEN-NS1-PAD obtained with the naked eye, scanner, and a smartphone camera was 200, 46.7, and 74.8 ng mL, respectively. The repeatability, reproducibility, and stability of the DEN-NS1-PAD were also evaluated. High true specificity and sensitivity in the serum of pediatric patients were observed. These evaluation results confirm that the DEN-NS1-PAD can potentially be used in point-of-care dengue diagnostics, which can significantly impact on the spreading of mosquito-borne diseases, which are likely to become more prevalent with the effects of global warming. Graphical Abstract.

Dengue NS1 detection in pediatric serum using microfluidic paper-based analytical devices.

This study aimed to evaluate a microfluidic paper-based analytical device (DEN-NS1-PAD) based on a rapid NS1 antigen test for diagnosing dengue at the point of care.

Clinical evaluation of a developed paper-based Dengue NS1 rapid diagnostic test for febrile illness patients.

Update diagnostic methods play essential roles in dealing with the current global malaria situation and decreasing malaria incidence.

Article	Year
Dengue NS1 detection in pediatric serum using microfluidic paper-based analytical devices. Analytical and bioanalytical chemistry\| PubMed ID: 32166444	2020
Clinical evaluation of a developed paper-based Dengue NS1 rapid diagnostic test for febrile illness patients. International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases\| PubMed ID: 33991681	2021
Malaria diagnostic update: From conventional to advanced method. Journal of clinical laboratory analysis\| PubMed ID: 35247002	2022

Muhammad Hatta Prabowo

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