optical-clearing-labeling-for-light-sheet-fluorescence-microscopy

Optical Clearing and Labeling for Light-sheet Fluorescence Microscopy in Large-scale Human Brain Imaging

Despite the numerous clearing techniques that emerged in the last decade, processing postmortem human brains remains a challenging task due to its ...

University of Florence

The microscopic visualization of nanoparticles in plants is crucial to elucidate the mechanisms of their uptake through the cell wall and plasma membrane and to localize the possible sites of their extracellular or intracellular accumulation. Lignin nanocarriers are polymeric hollow nanocapsules able to contain and transport several bioactive substances inside plant tissues. We describe here a method for the preparation of Fluorol Yellow 088-labeled lignin nanocapsules that allow their localization in plant organs and tissues by fluorescence microscopy.

Fluorescent Labeling of Lignin Nanocapsules with Fluorol Yellow 088.

Accurate labeling of specific layers in the human cerebral cortex is crucial for advancing our understanding of neurodevelopmental and neurodegenerative disorders. Leveraging recent advancements in ultra-high resolution  MRI, we present a novel semi-supervised segmentation model capable of identifying supragranular and infragranular layers in  MRI with unprecedented precision. On a dataset consisting of 17 whole-hemisphere  scans at 120 m, we propose a multi-resolution U-Nets framework (MUS) that integrates global and local structural information, achieving reliable segmentation maps of the entire hemisphere, with Dice scores over 0.8 for supra- and infragranular layers. This enables surface modeling, atlas construction, anomaly detection in disease states, and cross-modality validation, while also paving the way for finer layer segmentation. Our approach offers a powerful tool for comprehensive neuroanatomical investigations and holds promise for advancing our mechanistic understanding of progression of neurodegenerative diseases.

Article	Year
Fluorescent Labeling of Lignin Nanocapsules with Fluorol Yellow 088. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 36152265	2023
Segmentation of supragranular and infragranular layers in ultra-high resolution 7T MRI of the human cerebral cortex. bioRxiv : the preprint server for biology\| PubMed ID: 38106176	2023

Franco Cheli

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