quantifying-inferior-vena-cava-compliance-distensibility-an-vivo

Quantifying Inferior Vena Cava Compliance and Distensibility in an <i>In Vivo</i> Ovine Model Using 3D Angiography

Synthetic vascular grafts overcome some challenges of allografts, autografts, and xenografts but are often more rigid and less compliant than the native ...

wireless-telemetry-device-implantation-fontan-ovine-model-for

Wireless Telemetry Device Implantation in a Fontan Ovine Model for Continuous and Long-Term Hemodynamic Monitoring

While the Fontan procedure drastically improves life expectancy for patients with single ventricle, it is well recognized that the resulting circulation ...

surgical-implantation-wireless-system-for-portal-vein-pressure

Surgical Implantation of a Wireless System for Portal Vein Pressure Monitoring in a Chronic Fontan Ovine Model

The Fontan circulation is characterized by nonpulsatile pulmonary blood flow and chronic systemic venous congestion, which has been associated with ...

Nationwide Children's Hospital

Abigail Wexner Research Institute at Nationwide Children's Hospital

Patients with single ventricle heart defects requires a series of staged open-heart procedures, termed Fontan palliation. However, while lifesaving, these operations are associated with significant morbidity and early mortality. The attendant complications are thought to arise in response to the abnormal hemodynamics induced by Fontan palliation, although the pathophysiology underlying these physicochemical changes in cardiovascular and other organs remain unknown. Here, we investigated the microRNA (miRNA) content in serum and serum-derived extracellular vesicles (EVs) by sequencing small RNAs from a physiologically relevant sheep model of the Fontan operation. The differential expression analysis identified the enriched miRNA clusters in (1) serum vs. serum-derived EVs and (2) pre-Fontan EVs vs. post-Fontan EVs. Metascape analysis showed that the overexpressed subset of EV miRNAs by Fontan procedure target liver-specific cells, underscoring a potentially important pathway involved in the liver dysfunction that occurs as a consequence of Fontan palliation. We also found that post-Fontan EV miRNAs were associated with senescence and cell death, whereas pre-Fontan EV miRNAs were associated with stem cell maintenance and epithelial-to-mesenchymal transition. This study shows great potential to identify novel circulating EV biomarkers from Fontan sheep serum that may be used for the diagnosis, prognosis, and therapeutics for patients that have undergone Fontan palliation.

Computational analysis of serum-derived extracellular vesicle miRNAs in juvenile sheep model of single stage Fontan procedure.

Our goal was to conduct a hemodynamic analysis of a novel animal model of Fontan physiology. Poor late-term outcomes in Fontan patients are believed to arise from Fontan-induced hemodynamics, but the mechanisms remain poorly understood. Recent advances in surgical experimentation have resulted in the development of a chronic sheep model of Fontan physiology; however, detailed analysis of this model is lacking.

ArticleTotal : 3	Year
Publication title Cited by 2	2024
Publication title Cited by 1	2025
Publication title	2025

Article	Year
Computational analysis of serum-derived extracellular vesicle miRNAs in juvenile sheep model of single stage Fontan procedure. Extracellular vesicle\| PubMed ID: 36330420	2022
Investigation of a chronic single-stage sheep Fontan model. JTCVS open\| PubMed ID: 39534321	2024

Adrienne Morrison

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