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Field Collection and Laboratory Maintenance of Canopy-Forming Giant Kelp to Facilitate Restoration

Canopy-forming kelps are essential foundation species, supporting biodiversity and providing ecosystem services valued at more than USD$500 billion ...

University of California, Irvine

Predicting the outcome of future climate change requires an understanding of how alterations in multiple environmental factors manifest in natural communities and affect ecosystem functioning. We conducted an in situ, fully factorial field manipulation of CO2 and temperature on a rocky shoreline in southeastern Alaska, USA. Warming strongly impacted functioning of tide pool systems within one month, with the rate of net community production (NCP) more than doubling in warmed pools under ambient CO2 levels relative to initial NCP values. However, in pools with added CO2, NCP was unaffected by warming. Productivity responses paralleled changes in the carbon-to-nitrogen ratio of a red alga, the most abundant primary producer species in the system, highlighting the direct link between physiology and ecosystem functioning. These observed changes in algal physiology and community productivity in response to our manipulations indicate the potential for natural systems to shift rapidly in response to changing climatic conditions and for multiple environmental factors to act antagonistically.

Warming and Elevated CO2 Interact to Drive Rapid Shifts in Marine Community Production.

Intraspecific differentiation across a steep environmental gradient depends on the relative influences of evolutionary, organismal, and environmental processes. But steep environmental gradients may be nested within larger-scale, regional conditions that could influence these processes at the local scale. Therefore, we hypothesized that phenotypic differentiation along a steep environmental gradient would vary among regions. To test this hypothesis, we conducted a reciprocal transplant experiment on rocky intertidal shores, a habitat characterized by gradients in abiotic and biotic stress, in three regions of the Gulf of Maine. We used the ubiquitous and ecologically important rockweed species Fucus vesiculosus to quantify differentiation in growth, tissue nitrogen, and nitrogen productivity between upper and lower intertidal individuals. We found that phenotypic differentiation between tide heights varied among traits and regions. Although tissue nitrogen did not vary among any treatment combinations, growth and nitrogen productivity response were region specific. A strong effect of transplant height was found in all regions; however, an effect of home (source) height was only detectable in the central Gulf of Maine. Our study reveals that intraspecific responses to steep environmental gradients vary among populations, but the mechanisms underlying these patterns remain unknown. Given the roles that rockweeds play as food and habitat, these in situ patterns of growth and nitrogen productivity could have important community- and ecosystem-level consequences.

Interactive effects of large- and local-scale environmental gradients on phenotypic differentiation.

The co-occurrence of several species, all of which share similar resource requirements, remains a paradox in ecology. Here, I evaluated resource use along multiple environmental axes to understand the potential for niche partitioning and complementarity in a guild of suspension-feeding rocky shore invertebrates. I focused on the mussels Perna canaliculus, Mytilus galloprovincialis, Aulacomya maoriana, and Xenostrobus pulex, all of which coexist along the coastline of the South Island of New Zealand. I quantified the mussels' distributions, both vertically on the shore and within the three-dimensional mussel bed matrix, and used carbon (δC) and nitrogen (δN) stable isotope ratios to compare species' diets. Mussels exhibited niche partitioning along all resource axes. Given the mussels' use of different spatial and food resources, I evaluated the potential for complementarity with respect to mussels' roles as mediators of an important spatial subsidy, carbon inputs from the nearshore ocean into rocky-shore ecosystems. In these systems, mussels are basal species, capturing and consuming particulate organic matter in the ocean and making it available for local consumption within the benthic community. I found that mussel diversity matters; even the most productive species-Perna canaliculus-only contributed about half of the mussel-mediated carbon that accumulated over a year. Multiple co-occurring species are, therefore, likely to provide more carbon at the base of the intertidal food web than any one species living independently, and complementarity in resource use along multiple environmental axes is likely an important mechanism underlying this relationship.

Complementarity in spatial subsidies of carbon associated with resource partitioning along multiple niche axes.

It is critical to understand how human modifications of Earth's ecosystems are influencing ecosystem functioning, including net and gross community production (NCP and GCP, respectively) and community respiration (CR). These responses are often estimated by measuring oxygen production in the light (NCP) and consumption in the dark (CR), which can then be combined to estimate GCP. However, the method used to create "dark" conditions-either experimental darkening during the day or taking measurements at night-could result in different estimates of respiration and production, potentially affecting our ability to make integrative predictions. We tested this possibility by measuring oxygen concentrations under daytime ambient light conditions, in darkened tide pools during the day, and during nighttime low tides. We made measurements every 1-3 months over one year in southeastern Alaska. Daytime respiration rates were substantially higher than those measured at night, associated with higher temperature and oxygen levels during the day and leading to major differences in estimates of GCP calculated using daytime versus nighttime measurements. Our results highlight the potential importance of measuring respiration rates during both day and night to account for effects of temperature and oxygen-especially in shallow-water, constrained systems-with implications for understanding the impacts of global change on ecosystem metabolism.

Accounting for variation in temperature and oxygen availability when quantifying marine ecosystem metabolism.

Human impacts on ecosystems are resulting in unprecedented rates of biodiversity loss worldwide. The loss of species results in the loss of the multiple roles that each species plays or functions (i.e., "ecosystem multifunctionality") that it provides. A more comprehensive understanding of the effects of species on ecosystem multifunctionality is necessary for assessing the ecological impacts of species loss. We studied the effects of two dominant intertidal species, a primary producer (the seaweed ) and a consumer (the shellfish ), on 12 ecosystem functions in a coastal ecosystem, both in undisturbed tide pools and following the removal of the dominant producer. We modified analytical methods used in biodiversity-multifunctionality studies to investigate the potential effects of individual dominant species on ecosystem function. The effects of the two dominant species from different trophic levels tended to differ in directionality (+/-) consistently (92% of the time) across the 12 individual functions considered. Using averaging and multiple threshold approaches, we found that the dominant consumer-but not the dominant producer-was associated with ecosystem multifunctionality. Additionally, the relationship between abundance and multifunctionality differed depending on whether the dominant producer was present, with a negative relationship between the dominant consumer and ecosystem function with the dominant producer present compared to a non-significant, positive trend where the producer had been removed. Our findings suggest that interactions among dominant species can drive ecosystem function. The results of this study highlight the utility of methods previously used in biodiversity-focused research for studying functional contributions of individual species, as well as the importance of species abundance and identity in driving ecosystem multifunctionality, in the context of species loss.

Article	Year
Warming and Elevated CO2 Interact to Drive Rapid Shifts in Marine Community Production. PloS one\| PubMed ID: 26714167	2015
Interactive effects of large- and local-scale environmental gradients on phenotypic differentiation. Ecology\| PubMed ID: 32542682	2020
Complementarity in spatial subsidies of carbon associated with resource partitioning along multiple niche axes. Oecologia\| PubMed ID: 32556590	2020
Accounting for variation in temperature and oxygen availability when quantifying marine ecosystem metabolism. Scientific reports\| PubMed ID: 35039551	2022
The functional effects of a dominant consumer are altered following the loss of a dominant producer. Ecology and evolution\| PubMed ID: 37546568	2023

Matthew E. S. Bracken

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