isolation-umbilical-cord-derived-mesenchymal-stem-cells-with-high

Isolation of Umbilical Cord-Derived Mesenchymal Stem Cells with High Yields and Low Damage

Mesenchymal stem cells (MSCs) are a population of multipotent cells with remarkable regenerative and immunomodulatory properties. Wharton's jelly (WJ) ...

Beijing University of Chinese Medicine

Nucleosides, organic acids, and amino acids separated from  are well known for their nutritional value and flavor. However, how to increase their content in a better way has been a challenge. In this study, the effect of adding jujube kernel on the active components of  was investigated by untargeted metabolomics using UPLC-MS/MS. A total of 1,243 metabolites were identified, of which 262 metabolites (21.078%) were organic acids and derivatives, 245 metabolites (19.71%) were lipids and lipid-like molecules, and 26 metabolites (2.092%) were nucleosides, nucleotides, and analogues. Subsequently, differential metabolites between groups were screened by the orthogonal partial least squares-discriminant analysis model, which showed that 256 metabolites were identified as significantly different for the positive ion model and 149 for the negative ion model. Moreover, significant differential metabolites (VIP > 1,  < 0.05) in annotation of kyoto encyclopedia of genes and genomes pathway were investigated, which showed that ABC transporters were the most commonly observed transporters, followed by pyrimidine metabolism and purine metabolism. The results indicated that the main components of jujube kernel might be conducive to the accumulation of nucleoside organic acids and amino acid metabolites in . These results provide important information for the understanding of more suitable way for cultivation of .

Untargeted metabolomics reveals sour jujube kernel benefiting the nutritional value and flavor of .

Datura Metel L. has been used to treat psoriasis in China for a long time. The effect of extracts from Datura Metel L. for Psoriasis has not been previously confirmed. This study aimed to evaluate the efficacy of extracts from Datura Metel L. for patients with psoriasis.

Efficacy of extracts from Datura Metel L. for Psoriasis: a meta-analysis of case series and single-arm studies.

The heart is the central organ of the circulatory system, and its proper development is vital to maintain human life. As fetal heart development is complex and poorly understood, we use single-cell RNA sequencing to profile the gene expression landscapes of human fetal hearts from the four-time points: 8, 10, 11, 17 gestational weeks (GW8, GW10, GW11, GW17), and identified 11 major types of cells: erythroid cells, fibroblasts, heart endothelial cells, ventricular cardiomyocytes, atrial cardiomyocytes, macrophage, DCs, smooth muscle, pericytes, neural cells, schwann cells. In addition, we identified a series of differentially expressed genes and signaling pathways in each cell type between different gestational weeks. Notably, we found that ANNEXIN, MIF, PTN, GRN signalling pathways were simple and fewer intercellular connections in GW8, however, they were significantly more complex and had more intercellular communication in GW10, GW11, and GW17. Notably, the interaction strength of OSM signalling pathways was gradually decreased during this period of time (from GW8 to GW17). Together, in this study, we presented a comprehensive and clear description of the differentiation processes of all the main cell types in the human fetal hearts, which may provide information and reference data for heart regeneration and heart disease treatment.

Article	Year
Untargeted metabolomics reveals sour jujube kernel benefiting the nutritional value and flavor of . Open life sciences\| PubMed ID: 37671097	2023
Efficacy of extracts from Datura Metel L. for Psoriasis: a meta-analysis of case series and single-arm studies. BMC complementary medicine and therapies\| PubMed ID: 37710189	2023
Single-cell RNA sequencing reveals the gene expression profile and cellular communication in human fetal heart development. Developmental biology\| PubMed ID: 38876166	2024

Xinlei Si

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