electronic-von-frey-associated-with-mouse-grimace-scale-to-assess

Electronic von Frey associated with the Mouse Grimace Scale to Assess Allodynia and Pain in <em>Trypanosoma evansi</em>-Infected Mice

Infectious disease pathogenesis is still a complex field to study. The course of several clinical signs, such as allodynia and pain, may be observed in ...

Santa Catarina State University

Experimental Trypanosoma evansi infection induces pain along with oxidative stress, prevented by COX-2 inhibition.

Ion exchange chromatography is a method that uses the different surface charges of trypanosomes and blood cells to separate them. This makes it possible to use molecular and immunological methods to diagnose or study these protozoans. DEAE-cellulose resin is commonly used to perform this method. The goal of this study was to compare three novel chromatographic resins designated as PURIFICA™ (Y-C2N®, Y-HONOH®, and Y-CNC3®). The resins were evaluated based on their ability to isolate the parasite, purification time, examination of parasite viability and morphology, and trypanosome recovery potential after passing through the columns. In terms of the evaluated parameters, there was no significant difference between DEAE-cellulose and the three tested resins in most experiments. However, PURIFICA™ (Y-C2N®, Y-HONOH®, and Y-CNC3®) resins are less expensive and easier to prepare than DEAE-Cellulose, making them an alternative for the purification of Trypanosoma evansi.

Article	Year
Experimental Trypanosoma evansi infection induces pain along with oxidative stress, prevented by COX-2 inhibition. Experimental parasitology\| PubMed ID: 36720379	2023
Comparative study of three novel ion exchange resins with DEAE-cellulose for the purification of Trypanosoma evansi. Analytical biochemistry\| PubMed ID: 37422062	2023

Gabriela Kaiser Borges

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