visualization-genetic-manipulation-dendrites-spines-mouse-cerebral

Visualization and Genetic Manipulation of Dendrites and Spines in the Mouse Cerebral Cortex and Hippocampus using <em>In utero</em> Electroporation

In utero electroporation (IUE) has become a powerful technique to study the development of different regions of the embryonic nervous system 1-5. To date ...

national institute for medical research

Nuclear architecture and chromatin reorganization have recently been shown to orchestrate gene expression and act as key players in developmental pathways. To investigate how regulatory elements in the mouse CD8 gene locus are arranged in space and in relation to each other, three-dimensional fluorescence in situ hybridization and chromosome conformation capture techniques were employed to monitor the repositioning of the locus in relation to its subchromosomal territory and to identify long-range interactions between the different elements during development. Our data demonstrate that CD8 gene expression in murine lymphocytes is accompanied by the relocation of the locus outside its subchromosomal territory. Similar observations in the CD4 locus point to a rather general phenomenon during T cell development. Furthermore, we show that this relocation of the CD8 gene locus is associated with a clustering of regulatory elements forming a tight active chromatin hub in CD8-expressing cells. In contrast, in nonexpressing cells, the gene remains close to the main body of its chromosomal domain and the regulatory elements appear not to interact with each other.

Article	Year
AANA journal\| PubMed ID: 15208969	2004
Nature\| PubMed ID: 15846349	2005
AANA journal\| PubMed ID: 16261854	2005
CD8 locus nuclear dynamics during thymocyte development. Journal of immunology (Baltimore, Md. : 1950)\| PubMed ID: 20404270	2010
Nature methods\| PubMed ID: 21297619	2011
Nature\| PubMed ID: 21331042	2011
Neuron\| PubMed ID: 21435554	2011

Donald M. Bell

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