A Rat Model of Compound Acne

Acne vulgaris is a prevalent chronic skin condition characterized by the presence of comedones, papules, and pustules on the face, neck, and chest. To ...

Beijing University of Chinese Medicine

To study the learning and memory ability, expressions of hippocampal N-methyl D-aspartate (NMDA) subunit NR2A and NR2B, and EphB2 receptor in fatigue rats, and to observe effects of Sini Powder, Shenghui Decoction, and Sihui Mixture on them.

[Effect of sihui mixture on the learning and memory ability, mRNA expressions of hippocampal NMDA subunit NR2A and NR2B, and EphB2 receptor in fatigue rats].

To explore the effects of the extract from Phyllanthus urinaria L. on hepatitis B virus (HBV) replication and expression in HBV transient transfection model in vitro.

Extract from Phyllanthus urinaria L. inhibits hepatitis B virus replication and expression in hepatitis B virus transfection model in vitro.

To investigate the mechanism of Sini Powder () decoction (SND) in the treatment of insomnia.

Sini powder () decoction alleviates mood disorder of insomnia by regulating cation-chloride cotransporters in hippocampus.

Post-traumatic stress disorder (PTSD) is manifested as a persistent mental and emotional condition after potentially life-threatening events. Different animal models of PTSD have been developed for neuro-pathophysiology and pharmacological evaluations. A single prolonged stress (SPS) induced animal model has demonstrated to result in specific neuro-endocrinological dysregulation, and behavior abnormalities observed in PTSD. However, animal studies of PTSD have mostly been performed at one time point after SPS exposure. To better understand the development of PTSD-like behaviors in the SPS animal model, and to identify an optimal period of study, we examined depressive behavior, anxiety-like behavior, physical activity and body weight in SPS model rats for two weeks. Our results confirmed the SPS-induced PTSD-like behavior and physical activity observed in previous studies, and indicated that the most pronounced symptomatic behavior changes were observed on day 1 and 7 after SPS exposure, which may involve stress-induced acute hormone changes and unclear secondary neurobiological changes, respectively. These results provide a solid basis for further investigation into the neuro-pathophysiology of or neuropharmacology for PTSD using the SPS rat model. However, for chronic (pharmacological) studies longer than 7 days, a prolonged PTSD animal model should be developed, perhaps using enhanced stimulation. 

Behavioral changes over time in post-traumatic stress disorder: Insights from a rat model of single prolonged stress.

Shenghui soup is a traditional Chinese herbal medicine used in clinic for the treatment of forgetfulness. In order to understanding the prescription principle, the effects of "tonifying qi and strengthening spleen" group (TQSS) including Poria cocos (Schw.) Wolf. and Panax ginseng C.A.Mey and "eliminating phlegm and strengthening intelligence" group (EPSI) composed of Polygala tenuifolia Willd., Acorus calamus L. and Sinapis alba L from the herb complex on neurite growth in PC12 cells, two disassembled prescriptions derived from Shenghui soup and their molecular mechanisms were investigated.

The prescriptions from Shenghui soup enhanced neurite growth and GAP-43 expression level in PC12 cells.

The mechanism of Wendan Decoction (WDD) against Generalized Anxiety Disorder (GAD) was predicted by network pharmacology and validated by in vivo and in vitro experiments.

Network and Experimental Pharmacology to Decode the Action of Wendan Decoction Against Generalized Anxiety Disorder.

This study investigated the possible interaction between gut flora and miRNAs and the effect of both on anxiety disorders. The model group was induced with chronic restraint stress (CRS) and each group was tested for anxiety-like behaviour by open field test and elevated plus maze test. Meanwhile, the gut flora was analysed by 16S rRNA high-throughput sequencing. The miRNAs in hippocampus were analysed by high-throughput sequencing, and the key miRNAs were obtained by using the method of bioinformatics analysis. PCR was used to verify the significantly related key miRNAs. Spearman correlation analysis was used to explore the correlation between behaviour, key miRNAs and differential gut microbiota. The 16S rRNA high-throughput sequencing result showed that the gut flora was dysregulated in the model group. In particular, Verrucomicrobia, Akkermansia, Anaerostipes, Ralstonia, Burkholderia and Anaeroplasma were correlated with behaviour. The results of miRNA high-throughput sequencing analysis and bioinformatics analysis showed that 7 key miRNAs influenced the pathogenesis of anxiety, and qRT-PCR results were consistent with the high-throughput sequencing results. Mmu-miR-543-3p and mmu-miR-26a-5p were positively correlated with Verrucomicrobia, Akkermansia and Anaerostipes. Therefore, we infer that chronic stress caused the decrease of Akkermansia abundance, which may aggravate the decrease of mmu-miR-543-3p and mmu-miR-26a-5p expression, leading to the increase of SLC1A2 expression. In conclusion, gut flora has played an important influence on anxiety with changes in miRNAs.

Article	Year
[Effect of sihui mixture on the learning and memory ability, mRNA expressions of hippocampal NMDA subunit NR2A and NR2B, and EphB2 receptor in fatigue rats]. Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine\| PubMed ID: 21608225	2011
Extract from Phyllanthus urinaria L. inhibits hepatitis B virus replication and expression in hepatitis B virus transfection model in vitro. Chinese journal of integrative medicine\| PubMed ID: 25869593	2015
Sini powder () decoction alleviates mood disorder of insomnia by regulating cation-chloride cotransporters in hippocampus. Chinese journal of integrative medicine\| PubMed ID: 26597287	2015
Behavioral changes over time in post-traumatic stress disorder: Insights from a rat model of single prolonged stress. Behavioural processes\| PubMed ID: 26772783	2016
The prescriptions from Shenghui soup enhanced neurite growth and GAP-43 expression level in PC12 cells. BMC complementary and alternative medicine\| PubMed ID: 27646829	2016
Network and Experimental Pharmacology to Decode the Action of Wendan Decoction Against Generalized Anxiety Disorder. Drug design, development and therapy\| PubMed ID: 36193286	2022
Regulation of hippocampal miRNA expression by intestinal flora in anxiety-like mice. European journal of pharmacology\| PubMed ID: 39369876	2024

Yue-Han Song

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