quasistatic-mechanical-testing-for-computer-aided-design

Quasistatic Mechanical Testing for Computer-Aided Design and Manufacturing Occlusal Veneers Cemented to Milled Dentin Analog Material

Under current minimally invasive treatment regimes, minor tooth preparation and thinner biomimetic ceramic restoration are used to preserve the restored ...

Queen Mary University of London

The aim of this study was to test the modulus of elasticity () across the interfaces of yttria stabilized zirconia (YTZP) / veneer multilayers using nanoindentation.

Interfacial modulus mapping of layered dental ceramics using nanoindentation.

Recycling implants: a sustainable solution for musculoskeletal research.

Osteoporosis often leads to fragility fractures of the hip, resulting in impaired quality of life and increased mortality. Augmenting the proximal femur could be an attractive option for prevention of fracture or fixation device failure. We describe a tissue engineering based strategy to enhance long-term bone formation in the femoral neck of osteoporotic rats by locally delivering bioactive molecules; recombinant human bone morphogenic protein-2 (rhBMP-2), and zoledronic acid (ZA) by using a calcium sulfate/hydroxyapatite (CaS/HA) biomaterial. A defect was created by reaming the femoral neck canal of osteoporotic (OVX) rats and they were treated as follows: G1. Empty, G2. CaS/HA, G3. CaS/HA+Systemic ZA, G4. CaS/HA+Local ZA, and G5. CaS/HA+Local ZA+rhBMP-2. Bone formation was evaluated 6 months after treatment. Further, radioactively labeled C-ZA was used to study the bioavailability of ZA at the defect location, which was determined by using scintillation counting. Micro-CT indicated significantly higher bone volume in groups G4 and G5 compared with the other treatment groups. This was confirmed qualitatively by histological assessment. Addition of rhBMP-2 gave no additional benefit in this model. Local delivery of ZA performed better than systemic administration of ZA. Mechanical testing showed no differences between the groups, likely reflecting that the addition of bioactive molecules had limited effect on cortical bone or the choice of mechanical testing setup was not optimal. Scintillation counting revealed higher amounts of C-ZA present in the treated leg of G4 compared with its contralateral control and compared with G3, indicating that local ZA delivery can be used to achieve high local concentrations without causing a systemic effect. This long-term study shows that local delivery of ZA using a CaS/HA carrier can regenerate cancellous bone in the femoral neck canal and has clear implications for enhancing implant integration and fixation in fragile bone.

Long-Term Response to a Bioactive Biphasic Biomaterial in the Femoral Neck of Osteoporotic Rats.

HA-mineralised composite electrospun scaffolds have been introduced for bone regeneration due to their ability to mimic both morphological features and chemical composition of natural bone ECM. Micro-sized HA is generally avoided in electrospinning due to its reduced bioactivity compared to nano-sized HA due to the lower surface area. However, the high surface area of nanoparticles provides a very high surface energy, leading to agglomeration. Thus, the probability of nanoparticles clumping leading to premature mechanical failure is higher than for microparticles at higher filler content. In this study, two micron-sized hydroxyapatites were investigated for electrospinning with PLA at various contents, namely spray dried HA (HA1) and sintered HA (HA2) particles to examine the effect of polymer concentration, filler type and filler concentration on the morphology of the scaffolds, in addition to the mechanical properties and bioactivity. SEM results showed that fibre diameter and surface roughness of 15 and 20 wt% PLA fibres were significantly affected by incorporation of either HA. The apatite precipitation rates for HA1 and HA2-filled scaffolds immersed in simulated body fluid (SBF) were similar, however, it was affected by the fibre diameter and the presence of HA particles on the fibre surface. Degradation rates of HA2-filled scaffolds in vitro over 14 days was lower than for HA1-filled scaffolds due to enhanced dispersion of HA2 within PLA matrix and reduced cavities in PLA/HA2 interface. Finally, increasing filler surface area led to enhanced thermal stability as it reduced thermal degradation of the polymer.

Optimising micro-hydroxyapatite reinforced poly(lactide acid) electrospun scaffolds for bone tissue engineering.

There is a pressing clinical need to develop cell-based bone therapies due to a lack of viable, autologous bone grafts and a growing demand for bone grafts in musculoskeletal surgery. Such therapies can be tissue engineered and cellular, such as osteoblasts, combined with a material scaffold. Because mesenchymal stem cells (MSCs) are both available and fast growing compared to mature osteoblasts, therapies that utilize these progenitor cells are particularly promising. We have developed a nanovibrational bioreactor that can convert MSCs into bone-forming osteoblasts in two- and three-dimensional, but the mechanisms involved in this osteoinduction process remain unclear. Here, to elucidate this mechanism, we use increasing vibrational amplitude, from 30 nm (N30) to 90 nm (N90) amplitudes at 1000 Hz and assess MSC metabolite, gene, and protein changes. These approaches reveal that dose-dependent changes occur in MSCs' responses to increased vibrational amplitude, particularly in adhesion and mechanosensitive ion channel expression and that energetic metabolic pathways are activated, leading to low-level reactive oxygen species (ROS) production and to low-level inflammation as well as to ROS- and inflammation-balancing pathways. These events are analogous to those that occur in the natural bone-healing processes. We have also developed a tissue engineered MSC-laden scaffold designed using cells' mechanical memory, driven by the stronger N90 stimulation. These mechanistic insights and cell-scaffold design are underpinned by a process that is free of inductive chemicals.

Nanovibrational Stimulation of Mesenchymal Stem Cells Induces Therapeutic Reactive Oxygen Species and Inflammation for Three-Dimensional Bone Tissue Engineering.

Bone is a dynamic tissue with a quarter of the trabecular and a fifth of the cortical bone being replaced continuously each year in a complex process that continues throughout an individual's lifetime. Bone has an important role in homeostasis of minerals with non-stoichiometric hydroxyapatite bone mineral forming the inorganic phase of bone. Due to its crystal structure and chemistry, hydroxyapatite (HA) and related apatites have a remarkable ability to bind molecules. This review article describes the accretion of trace elements in bone mineral giving a historical perspective. Implanted HA particles of synthetic origin have proved to be an efficient recruiting moiety for systemically circulating drugs which can locally biomodulate the material and lead to a therapeutic effect. Bone mineral and apatite however also act as a waste dump for trace elements and drugs, which significantly affects the environment and human health. Cite this article:  2020;9(10):709-718.

Bone mineral as a drug-seeking moiety and a waste dump.

Characterise the spatiotemporal trabecular and cortical bone responses to complete spinal cord injury (SCI) in young rats.

Time course changes to structural, mechanical and material properties of bone in rats after complete spinal cord injury.

Phenotypic plasticity enables development to produce multiple phenotypes in response to environmental conditions. Plasticity driven variation has been suggested to play a key role in adaptive divergence, and plasticity itself can evolve. However, the interaction of plasticity with the multiple levels involved with adaptive divergence is less understood. For example, sexual dimorphism can contribute adaptive variation through ecological sexual dimorphism (ESD), but the contribution of plasticity to this phenomenon is unknown. Therefore, to determine the potential contribution of plasticity to ESD, we used the adaptive radiation of Malawi cichlids. Two mouthbrooding species ( and  "Red Cheek") with differences in foraging tactics underwent foraging experiments using benthic and limnetic treatments while accounting for sex. Plasticity in craniofacial shape and three functionally important traits were measured. Plasticity was shown, but without any sex-based differences in shape. However, for mechanical advantage traits of the mandible sex by diet interactions were found. This suggests that ESD, may be influenced by phenotypic plasticity that diverges between sexes. Given the involvement of the mandible in parental care in cichlids this may indicate that sexual divergence in plasticity may trade-off against maternal care tactics.

Article	Year
Interfacial modulus mapping of layered dental ceramics using nanoindentation. The journal of advanced prosthodontics\| PubMed ID: 28018566	2016
Recycling implants: a sustainable solution for musculoskeletal research. Acta orthopaedica\| PubMed ID: 31902268	2020
Long-Term Response to a Bioactive Biphasic Biomaterial in the Femoral Neck of Osteoporotic Rats. Tissue engineering. Part A\| PubMed ID: 32242474	2020
Optimising micro-hydroxyapatite reinforced poly(lactide acid) electrospun scaffolds for bone tissue engineering. Journal of materials science. Materials in medicine\| PubMed ID: 32253587	2020
Nanovibrational Stimulation of Mesenchymal Stem Cells Induces Therapeutic Reactive Oxygen Species and Inflammation for Three-Dimensional Bone Tissue Engineering. ACS nano\| PubMed ID: 32658450	2020
Bone mineral as a drug-seeking moiety and a waste dump. Bone & joint research\| PubMed ID: 33399475	2020
Time course changes to structural, mechanical and material properties of bone in rats after complete spinal cord injury. Journal of musculoskeletal & neuronal interactions\| PubMed ID: 35642701	2022
Functional trait plasticity diverges between sexes in African cichlids: A contribution toward ecological sexual dimorphism? Ecology and evolution\| PubMed ID: 38034329	2023

K. Elizabeth Tanner

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