bridging-the-bio-electronic-interface-with-biofabrication

Bridging the Bio-Electronic Interface with Biofabrication

Advancements in lab-on-a-chip technology promise to revolutionize both research and medicine through lower costs, better sensitivity, portability, and ...

University of Maryland 

The emergence of bacteria that evade antibiotics has accelerated research on alternative approaches that do not target cell viability. One such approach targets cell-cell communication networks mediated by small molecule signaling. In this report, we assemble biological nanofactories within a bioMEMS device to capture and manipulate the behavior of quorum sensing (QS) bacteria as a step toward modifying small molecule signaling. Biological nanofactories are bio-inspired nanoscale constructs which can include modules with different functionalities, such as cell targeting, molecular sensing, product synthesis, and ultimately self-destruction. The biological nanofactories reported here consist of targeting, sensing, synthesis and, importantly, assembly modules. A bacteria-specific antibody constitutes the targeting module while a genetically engineered fusion protein contains the sensing, synthesis and assembly modules. The nanofactories are assembled on chitosan electrodeposited within a microchannel of the bioMEMS device; they capture QS bacteria in a spatially selective manner and locally synthesize and deliver the "universal" small signaling molecule autoinducer-2 (AI-2) at the captured cell surface. The nanofactory based AI-2 delivery is demonstrated to alter the progression of the native AI-2 based QS response of the captured bacteria. Prospects are envisioned for utilizing our technique as a test-bed for understanding the AI-2 based QS response of bacteria as a means for developing the next generation of antimicrobials.

Article	Year
Lab on a chip\| PubMed ID: 17102845	2006
Biotechnology and bioengineering\| PubMed ID: 17625789	2008
Lab on a chip\| PubMed ID: 18305860	2008
Biomedical microdevices\| PubMed ID: 18626771	2008
Lab on a chip\| PubMed ID: 20024051	2010
Biological nanofactories facilitate spatially selective capture and manipulation of quorum sensing bacteria in a bioMEMS device. Lab on a chip\| PubMed ID: 20390130	2010
Pharmaceutical statistics\| PubMed ID: 20872620	2010
Biometrical journal. Biometrische Zeitschrift\| PubMed ID: 21154898	2010
Lab on a chip\| PubMed ID: 21629950	2011
Biotechnology journal\| PubMed ID: 22213675	2012
Biomaterials\| PubMed ID: 22507453	2012
Journal of biopharmaceutical statistics\| PubMed ID: 22651110	2012

Xiaolong Luo

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