in-vitro-modeling-down-syndrome-neurogenesis-using-human-induced

<em>In Vitro</em> Modeling of Down Syndrome Neurogenesis Using Human-Induced Pluripotent Stem Cells

Down syndrome (DS), caused by an extra copy of chromosome 21, is a leading cause of intellectual disability. One of the key factors contributing to this ...

S.P. Pune University Complex

Regional Centre for Biotechnology

Human mouse chimeric models are valuable tools to develop in-vivo disease models. However, in-vivo detection of human cells limits their analysis. To facilitate in-vivo modeling of Down syndrome (DS), we generated a stable AAVS1-EGFP isogenic pair of DS human iPSCs by zinc finger mediated genetic engineering of the AAVS1 locus. These lines overcome the limitation of reporter human iPSCs generated using random integration, which may not express reporter gene in all tissues due to heterochromatin-induced gene silencing. These reporter cell lines provide a valuable tool to facilitate in-vivo tracking of the graft cell integration, differentiation, and distinction from host cells.

Nishant Singhal

Biography

JoVE Journal Publications

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