plasmonic-photothermal-cancer-therapy-nanoparticle-embedded-tumor

Plasmonic Photothermal Cancer Therapy: Nanoparticle-embedded Tumor-tissue-mimicking Phantoms for Visualizing Photothermal Temperature Distribution

Plasmonic photothermal therapy (PPTT), an emerging cancer treatment, involves delivering nanoparticles (NPs) to a tumor, followed by near-infrared (NIR) ...

CSIR-Central Scientific Instruments Organisation

Academy of Scientific and Innovative Research (AcSIR)

Multifunctional nanocomposites are of potential use to achieve complete tumor elimination and, thus, to avoid tumor recurrence. Herein, polydopamine (PDA)-based gold nanoblackbodies (AuNBs) loaded with indocyanine green (ICG) and Doxorubicin (DOX) termed as A-P-I-D nanocomposite were investigated for multimodal plasmonic photothermal-photodynamic-chemotherapy. Upon near-infrared (NIR) irradiation, A-P-I-D nanocomposite showed enhanced photothermal conversion efficiency of 69.2% compared to bare AuNBs (62.9%) due to the presence of ICG, along with ROS (O) generation as well as enhanced DOX release. On assessment of therapeutic effects on breast cancer (MCF-7) and melanoma (B16F10) cell lines, A-P-I-D nanocomposite showed significantly lower cell viabilities of 45.5% and 24% compared to 79.3% and 76.8% for AuNBs. Fluorescence images of stained cells revealed characteristic signs of apoptotic mode of cell death, with almost complete damage on A-P-I-D nanocomposite + NIR treated cells. Further, on evaluation of photothermal performance through breast tumor-tissue mimicking phantoms, A-P-I-D nanocomposite provided required thermal ablation temperatures within the tumor along with the potential for the elimination of residual cancerous cells through photodynamic therapy and chemotherapy. Overall, this study demonstrates that A-P-I-D nanocomposite + NIR provides better therapeutic outcome on cell lines and enhanced photothermal performance on breast tumor-tissue mimicking phantoms to be a promising agent for multimodal cancer therapy.

Gold nanoblackbodies-based multifunctional nanocomposite for multimodal cancer therapy.

Plasmonic photothermal therapy (PPTT) is a potential technique to treat tumors selectively. However, during PPTT, issue of high temperature region and damage to the surrounding healthy is still need to be resolved. Also, treatment of deeper tumors non-invasively is a challenge for PPTT. In this paper, the effect of periodic irradiation and incident beam radius (relative to tumor size) for various gold nanorods (GNRs) concentrations is investigated to avoid much higher temperatures region with limiting thermal damage to the surrounding healthy tissue during PPTT of subsurface breast tumors located at various depths. Lattice Boltzmann method is used to solve Pennes' bioheat model to compute the resulting photothermal temperatures for the subsurface tumor embedded with GNRs subjected to broadband near infrared radiation of intensity 1 W/cm. Computation revealed that low GNRs concentration leads to uniform internal heat generation than higher GNRs concentrations. The results show that deeper tumors, due to attenuation of incident radiation, show low temperature rise than shallower tumors. For shallower tumors situated 3 mm deep, 70% irradiation period resulted in around 20 °C reduction (110 °C-90 °C) of maximum temperature than that with the continuous irradiation. Moreover, 70% beam radius (i.e., beam radius as 70% of the tumor radius) causes less thermal damage to the nearby healthy tissue than 100% beam radius (i.e., beam radius equal to the tumor radius). The thermal damage within the healthy tissue is minimized to the 1 mm in radial direction and 3 mm in axial direction for 70% beam radius with 70% irradiation period. Overall, periodic heating and changing beam radius of the incident irradiation lead to reduce high temperature and limit healthy tissue damage. Hence, discussed results are useful for selection of the irradiation parameters for PPTT of sub-surface tumors.

Role of periodic irradiation and incident beam radius for plasmonic photothermal therapy of subsurface tumors.

Plasmonic photothermal therapy (PPTT) involves the use of nanoparticles and near-infrared radiation to attain a temperature above 50 °C within the tumor for its thermal damage. PPTT is largely explored for superficial tumors, and its potential to treat deeper subsurface tumors is dealt feebly, requiring the assessment of thermal damage for such tumors. In this paper, the extent of thermal damage is numerically analyzed for PPTT of invasive ductal carcinoma (IDC) situated at 3-9 mm depths. The developed numerical model is validated with suitable tissue-tumor mimicking phantoms. Tumor (IDC) embedded with gold nanorods (GNRs) is subjected to broadband near-infrared radiation. The effect of various GNRs concentrations and their spatial distributions [viz. uniform distribution, intravenous delivery (peripheral distribution) and intratumoral delivery (localized distribution)] are investigated for thermal damage for subsurface tumors situated at various depths. Results show that lower GNRs concentrations lead to more uniform internal heat generation, eventually resulting in uniform temperature rise. Also, the peripheral distribution of nanoparticles provides a more uniform spatial temperature rise within the tumor. Overall, it is concluded that PPTT has potential to induce thermal damage for subsurface tumors, at depths of upto 9 mm, by proper choice of nanoparticle distribution, dose/concentration and irradiation parameters based on the tumor location. Moreover, intravenous administration of nanoparticles seems a good choice for shallower tumors, while for deeper tumors, uniform distribution is required to attain the necessary thermal damage. In the future, the algorithm may be extended further, involving 3D patient-specific tumors and through mice model-based experiments.

Article	Year
Gold nanoblackbodies-based multifunctional nanocomposite for multimodal cancer therapy. International journal of pharmaceutics\| PubMed ID: 37302667	2023
Role of periodic irradiation and incident beam radius for plasmonic photothermal therapy of subsurface tumors. Journal of thermal biology\| PubMed ID: 38714147	2024
Assessment of thermal damage for plasmonic photothermal therapy of subsurface tumors. Physical and engineering sciences in medicine\| PubMed ID: 38753284	2024

Amit Kumar Shaw

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