rapid-detection-helicobacter-pylori-virulence-typing-using-quantum

Rapid Detection of <em>Helicobacter pylori</em> Virulence and Typing Using Quantum Dot Labeling Technology

We present a method based on quantum dot labeling technology for rapidly detecting the virulence of Helicobacter pylori and performing typing, aiming to ...

Southern Medical University

Lung cancer is a leading cause of cancer-related death worldwide. Cisplatin-based chemotherapy is the standard treatment for lung cancer, but chemoresistance and adverse effects especially cardiotoxicity limit its efficacy.

Dendrobine targeting JNK stress signaling to sensitize chemotoxicity of cisplatin against non-small cell lung cancer cells in vitro and in vivo.

To study the clinical features of children with carbapenem-resistant  (CRE) infection and the molecular characteristics of isolated strains.

Clinical features and molecular epidemiology of carbapenem-resistant  infection in children.

Nocardiosis demonstrates a temporal categorization that includes acute, subacute, and chronic stages alongside distinct typical localizations such as pulmonary, cutaneous, and disseminated forms. Disseminated nocardiosis, commonly caused by , , and , continues to result in substantial morbidity and mortality. Herein, we report a life-threatening disseminated nocardiosis caused by  in a patient with minimal change disease. This study emphasizes the difficulty in the diagnosis and treatment of unknown infections in clinical settings and highlights the important role played by laboratories in solving infectious diseases caused by rare pathogens.

Weathering the storm: diagnosis and treatment of a life-threatening disseminated  infection.

 is one of the most common causes of hospital-acquired infections, especially due to the emergence of the hypervirulent  (hvKp) strains. Multiple methods have been developed to discriminate hvKp strains from classical  (cKp) strains, such as the presence of candidate genes (, -344, , and ), high level of siderophore production, hypermucoviscosity phenotype,  Although the string test is commonly used to confirm the hypermucoviscosity of  strains, it is a method lacking rigidity and accuracy. Surface-enhanced Raman spectroscopy (SERS) coupled with machine learning algorithms has been widely used in discriminating bacterial pathogens with different phenotypes. However, the technique has not be applied to identify hypermucoviscous  (hmvKp) strains. In this study, we isolated a set of  strains from clinical samples, among which hmvKp strains ( = 10) and cKP strains ( = 10) were randomly selected to collect SESR spectra. Eight machine learning algorithms were recruited for model construction and spectral prediction in this study, among which support vector machine (SVM) outperforms all other algorithms with the highest prediction accuracy of hmvKp strains (5-fold cross validation = 99.07%). Taken together, this pilot study confirms that SERS, combined with machine learning algorithms, can accurately identify hmvKp strains, which can facilitate the fast recognition of hvKP strains when combined with relevant methods and biomarkers in clinical settings in the near future.

Identification of hypermucoviscous  strains  untargeted surface-enhanced Raman spectroscopy.

To assess the risk factors associated with Helicobacter pylori infection in the urban Chinese population.

Article	Year
Dendrobine targeting JNK stress signaling to sensitize chemotoxicity of cisplatin against non-small cell lung cancer cells in vitro and in vivo. Phytomedicine : international journal of phytotherapy and phytopharmacology\| PubMed ID: 30668397	2019
Clinical features and molecular epidemiology of carbapenem-resistant infection in children. Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics\| PubMed ID: 36036126	2022
Weathering the storm: diagnosis and treatment of a life-threatening disseminated infection. Frontiers in cellular and infection microbiology\| PubMed ID: 38881735	2024
Identification of hypermucoviscous strains untargeted surface-enhanced Raman spectroscopy. Analytical methods : advancing methods and applications\| PubMed ID: 39234672	2024
Risk factors associated with Helicobacter pylori infection in the urban population of China: A nationwide, multi-center, cross-sectional study. International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases\| PubMed ID: 40096882	2025

Li-Yan Zhang

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