endoscopic-ultrasound-based-differentiation-pancreatic-neuroendocrine

Endoscopic Ultrasound-Based Differentiation of Pancreatic Neuroendocrine Tumors and Pancreatic Duct Adenocarcinoma

Endoscopic ultrasound (EUS) is one of the most critical imaging modalities for evaluating pancreatic masses, providing high-resolution visualization of ...

allergic-bronchopulmonary-aspergillosis-aided-bronchoalveolar-lavage

Allergic Bronchopulmonary Aspergillosis Aided by Bronchoalveolar Lavage: A Case Report

This protocol establishes a clear primary goal: to develop a structured, actionable diagnostic workflow that enables accurate differentiation of allergic ...

Fudan University

Liver fibrosis is a global public health problem, and the activation of hepatic stellate cells (HSCs) is the main driving force for liver fibrosis. However, the activation mechanism of HSCs is still not fully understood. In this study, we screened out 854 differentially expressed genes [Log fold change absolute: log2 FC(abs) ≥ 1] in activated LX-2 cells. Subsequently, we performed functional analyses of these differentially expressed genes. Gene Ontology enrichment analysis showed that the target genes were mainly enriched in processes such as positive regulation of cell migration involved in sprouting angiogenesis, negative regulation of keratinocyte proliferation, and nuclear inclusion bodies. Kyoto Encyclopedia of Gene and Genome signaling pathway enrichment analysis revealed that dysregulated genes were involved in signaling pathways such as pantothenate and coenzyme A biosynthesis and riboflavin metabolism. The microarray results were validated by reverse transcription-quantitative polymerase chain reaction, which indicated that the microarray results were reliable and that the tripartite motif containing 15 (TRIM15) had the highest absolute value of LogFC. Additionally, the effect of TRIM15 on the proliferation, migration, and activation of LX-2 cells was assessed using overexpression plasmids and siRNA transfections. TRIM15 promoted the proliferation and migration of LX-2 cells and positively regulated the expression of α-smooth muscle actin and type I collagen. Collectively, the data revealed the gene expression profiles of quiescent and activated LX-2 cells and the involvement of TRIM15 in the activation of LX-2 cells. Hereby, TRIM15 could be a novel target of the HSC activation mechanism.

Knockdown of TRIM15 inhibits the activation of hepatic stellate cells.

Associations between metabolic risk factors and lung cancer remain elusive, and evidence on the linkage between non-alcoholic fatty liver disease (NAFLD) and pulmonary nodules is limited. This study sought to examine the independent association between NAFLD and the risk of pulmonary nodules.

Association between non-alcoholic fatty liver disease and the risk of pulmonary nodules in patients with intestinal polyps.

Dendrobine (DDB), an alkaloid isolated from the Chinese herb Dendrobium, has antioxidant and anti-inflammatory effects; however, whether DDB reduces oleic acid (OA)-induced lipid accumulation remains unclear. OA-induced lipid accumulation model of HepG2 cells were treated with DDB. Cellular lipid deposition was assessed by Oil Red O (ORO) staining and triglyceride and total cholesterol detection. RNA-Sequencing (RNA-seq), biological function analysis, and transcription factor (TFs) prediction were combined to identify key TF in the DDB-treated OA model. Finally, the roles of FOS and METTL14 were examined using a DDB-induced lipid accumulation model. DDB inhibited OA-induced lipid accumulation. We identified 895 differentially expressed genes (DEGs) that were mainly enriched in various biological processes of lipid synthesis and transport. Four transcription factors (SOX9, MLXIPL, FOS, and JUN) associated with lipid metabolism and FOS levels in the OA-induced lipid accumulation model after DDB treatment had the greatest changes in expression change. Overexpression of FOS alleviates the inhibitory effect of DDB on OA-induced lipid accumulation. METTL14 is a target gene of FOS, and simultaneous interference with METTL14 in cells with high FOS expression restored the alleviating effect of DDB on lipid accumulation. DDB alleviated OA-induced lipid accumulation by inhibiting the FOS/METTL14 pathway.

ArticleTotal : 2	Year
Publication title	2025
Publication title	2026

Article	Year
Knockdown of TRIM15 inhibits the activation of hepatic stellate cells. Journal of molecular histology\| PubMed ID: 34142270	2021
Association between non-alcoholic fatty liver disease and the risk of pulmonary nodules in patients with intestinal polyps. Journal of thoracic disease\| PubMed ID: 38983169	2024
Dendrobine alleviates oleic acid-induced lipid accumulation by inhibiting FOS/METTL14 pathway. Journal of molecular histology\| PubMed ID: 39136847	2024
Correction: Dendrobine alleviates oleic acid-induced lipid accumulation by inhibiting FOS/METTL14 pathway. Journal of molecular histology\| PubMed ID: 39298064	2024

Junpei Zhang

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