unilateral-lung-volume-analysis-using-micro-ct-for-enhanced

Unilateral Lung Volume Analysis Using Micro-CT for Enhanced Assessment of Pulmonary Fibrosis in Preclinical Models

Pulmonary fibrosis (PF) is a progressive and irreversible interstitial lung disease characterized by poor prognosis and limited treatment options. The ...

orthotopic-left-lung-transplantation-in-rats

Orthotopic Left Lung Transplantation in Rats

The rat left lung orthotopic transplantation model is an essential tool in lung transplant research. Although lung transplantation is a complex procedure, ...

establishment-evaluation-an-vitro-human-based-advanced-model

Establishment and Evaluation of an <em>In Vitro</em> Human-Based Advanced Model of Pulmonary Fibrosis

Pulmonary fibrosis is characterized by irreversible destruction of alveolar structure and excessive deposition of extracellular matrix. Although animal ...

The First Affiliated Hospital of Guangzhou Medical University

Guangzhou National Laboratory

Guangzhou Medical University

Pulmonary fibrosis is a severe lung interstitial disease characterized by the destruction of lung tissue structure, excessive activation and proliferation of fibroblasts, secretion and accumulation of a large amount of extracellular matrix (ECM), and impaired lung function. Due to the complexity of the disease, a suitable animal model to mimic human pulmonary fibrosis has not yet been established. Precision-cut lung slice (PCLS) has been a widely used in vitro method to study lung physiology and pathogenesis in recent years. This method is an in vitro culture technology at the level between organs and cells, because it can preserve the lung tissue structure and various types of airway cells in the lung tissue, simulate the in vivo lung environment, and conduct the observation of various interactions between cells and ECM. Therefore, PCLS can compensate for the limitations of other models such as cell culture. In order to explore the role of discoidin domain receptor 2 (DDR2) in pulmonary fibrosis, Ddr2 mice were successfully constructed. The Cre-LoxP system and PCLS technology were used to verify the deletion or knockdown of DDR2 in mouse PCLS. Transforming growth factor β1 (TGF-β1) can induce fibrosis of mouse PCLS in vitro, which can simulate the in vivo environment of pulmonary fibrosis. In the DDR2 knock down-PCLS in vitro model, the expression of various fibrosis-related factors induced by TGF-β1 was significantly reduced, suggesting that knocking down DDR2 can inhibit the formation of pulmonary fibrosis. The results provide a new perspective for the clinical study of DDR2 as a therapeutic target in pulmonary fibrosis.

[Application of precision-cut lung slice technology to study the role of DDR2 in pulmonary fibrosis].

Idiopathic pulmonary fibrosis is a progressive fibrotic lung disease with limited therapeutic options and high lethality, related to alveolar type II epithelial (ATII) cell dysregulation, the abnormal repair of alveolar epithelial cells and activation of fibroblasts promote the development of pulmonary fibrosis. Fatty acid binding protein 1 (FABP1) was significantly downregulated in the fibrotic state by proteomics screening in our previous date, and the ATII cell dysregulation can be mediated by FABP1 via regulating fatty acid metabolism and intracellular transport. The aim of this study was to evaluate the role and potential mechanism of FABP1 in the development of pulmonary fibrosis.

ArticleTotal : 3	Year
Publication title	2025
Publication title Cited by 1	2025
Publication title	2025

Article	Year
[Application of precision-cut lung slice technology to study the role of DDR2 in pulmonary fibrosis]. Sheng li xue bao : [Acta physiologica Sinica]\| PubMed ID: 37583038	2023
Therapeutic effects of fatty acid binding protein 1 in mice with pulmonary fibrosis by regulating alveolar epithelial regeneration. BMJ open respiratory research\| PubMed ID: 37940355	2023

Xihui Huang

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