identification-of-mycobacterium-species-by-dna-microarray-chip-method

Identification of <em>Mycobacterium</em> Species by DNA Microarray Chip Method

This study presents a DNA microarray chip method designed for the accurate identification of Mycobacterium species. By leveraging asymmetric PCR ...

Quanzhou First Hospital Affiliated to Fujian Medical University

Worldwide, cervical cancer is the fouth leading cause of deaths in gynecological oncology. Although the causes of cervical cancer have been extensively investigated, understanding of its exact pathogenesis remains incomplete.

Whole-exome and RNA sequencing reveal novel insights into the pathogenesis of HPV associated cervical cancer.

This study aimed to evaluate the prognostic value and biological function of small ubiquitin-like modifier 2 (SUMO2) in hepatocellular carcinoma (HCC). SUMO2 expression in HCC tissues was markedly higher than that in normal liver tissues, and patients with high SUMO2 expression had significantly shorter median overall survival than those with low SUMO2 expression. Furthermore, SUMO2 expression was closely correlated with lymph node metastasis and vascular invasion and was a predictor of poor prognosis. The knockdown of SUMO2 in two HCC cell lines (SMMC-7721 and Bel-7404) dramatically suppressed their proliferation, migration and invasion. Western blot analysis showed that the downregulation of SUMO2 significantly reduced the expression of Ki-67, matrix metalloproteinase-9 (MMP-9) and vascular endothelial growth factor (VEGF) in SMMC-7721 and Bel-7404 cells. Similarly, quantitative reverse transcription-PCR revealed consistently decreased expression of MMP-9 and VEGF. Our data suggest that SUMO2 promotes proliferation, migration and invasion of HCC cells via mechanisms involving MMP-9 and VEGF. Therefore, SUMO2 may be a prognostic factor and a promising therapeutic target for patients with HCC.

Downregulation of SUMO2 inhibits hepatocellular carcinoma cell proliferation, migration and invasion.

Gastric cancer (GC) is one hackneyed malignancy tumor accompanied by high death rate. DKC1 has been discovered to serve as a facilitator in several cancers. Additionally, it was discovered from one study that DKC1 displayed higher expression in GC tissues than in the normal tissues. Nevertheless, its role and regulatory mechanism in GC is yet to be illustrated. In this study, it was proved that DKC1 expression was upregulated in GC tissues through GEPIA and UALCAN databases. Moreover, we discovered that DKC1 exhibited higher expression in GC cells. Functional experiments testified that DKC1 accelerated cell proliferation, migration, and invasion in GC. Further investigation disclosed that the weakened cell proliferation, migration, and invasion stimulated by DKC1 knockdown can be reversed after TNFAIP6 overexpression. Lastly, through in vivo experiments, it was demonstrated that DKC1 strengthened tumor growth. In conclusion, our work uncovered that DKC1 aggravated GC cell migration and invasion through upregulating the expression of TNFAIP6. This discovery might highlight the function of DKC1 in GC treatment.

DKC1 aggravates gastric cancer cell migration and invasion through up-regulating the expression of TNFAIP6.

The vaginal microbiome (VM) is associated with human papillomavirus (HPV) infection and progression, but a thorough understanding of the relation between HPV infection, and VM needs to be elucidated. From August to December 2022, women who underwent routine gynecological examinations were screened for HPV infection. The distribution of HPV variants and clinical characteristics were collected. Then, a total of 185 participants were enrolled and divided into HPV-negative (HC), high-risk HPV (H), low-risk HPV (L), multiple high-risk HPV (HH), and mixed high-low risk HPV (HL) groups. Samples were collected from the mid-vagina of these 185 participants and sent for 16S rDNA sequencing (V3-V4 region). Among 712 HPV-positive women, the top 3 most frequently detected genotypes were HPV52, HPV58, and HPV16. Among 185 participants in the microbiology study, the β diversity of the HC group was significantly different from HPV-positive groups ( < 0.001). LEfSe analysis showed that  was a potential biomarker for H group, while  was for L group. Regarding HPV-positive patients, the α diversity of cervical lesion patients was remarkably lower than those with normal cervix ( < 0.05). Differential abundance analysis showed that  significantly reduced in cervical lesion patients ( < 0.001). Further community state type (CST) clustering displayed that CST IV was more common than other types in HC group ( < 0.05), while CST I was higher than CST IV in H group ( < 0.05). Different HPV infections had distinct vaginal microbiome features. HPV infection might lead to the imbalance of  spp. and cause cervical lesions.

Article	Year
Whole-exome and RNA sequencing reveal novel insights into the pathogenesis of HPV associated cervical cancer. Cancer biomarkers : section A of Disease markers\| PubMed ID: 31306105	2019
Downregulation of SUMO2 inhibits hepatocellular carcinoma cell proliferation, migration and invasion. FEBS open bio\| PubMed ID: 33989451	2021
DKC1 aggravates gastric cancer cell migration and invasion through up-regulating the expression of TNFAIP6. Functional & integrative genomics\| PubMed ID: 38376551	2024
Human papillomavirus molecular prevalence in south China and the impact on vaginal microbiome of unvaccinated women. mSystems\| PubMed ID: 39120153	2024

Jiang Yancheng

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