time-lapse-fluorescence-imaging-arabidopsis-root-growth-with-rapid

Time-lapse Fluorescence Imaging of Arabidopsis Root Growth with Rapid Manipulation of The Root Environment Using The RootChip

The root functions as the physical anchor of the plant and is the organ responsible for uptake of water and mineral nutrients such as nitrogen, ...

Carnegie Institution for Science

&#x8FD1; 150 &#x5E74;&#x6765;&#xFF0C;&#x5B83;&#x4E00;&#x76F4;&#x88AB;&#x516C;&#x8BA4;&#x7EC6;&#x80DE;&#x5F62;&#x72B6;&#x5F3A;&#x70C8;&#x5F71;&#x54CD;&#x7684;&#x6709;&#x4E1D;&#x5206;&#x88C2;&#x7684;&#x89E3;&#x7406;&#x9762; (&#x4F8B;&#x5982;&#xFF0C;&#x5C31;&#xFF0C;1863&#x5E74;) &#x65B9;&#x5411;&#x3002;&#x7136;&#x800C;&#xFF0C;&#x6211;&#x4EEC;&#x4ECD;&#x7136;&#x7406;&#x89E3;&#x4E0D;&#x592A;&#x4E86;&#x89E3;&#x7EC6;&#x80DE;&#x5F62;&#x72B6;&#x548C;&#x5207;&#x5272;&#x5E73;&#x9762;&#x65B9;&#x5411;&#x4E0A;&#x76AE;&#xFF0C;&#x5728;&#x591A;&#x8FB9;&#x5F62;&#x7EC6;&#x80DE;&#x51E0;&#x4F55;&#x56FE;&#x5F62;&#x6446;&#x8131;&#x591A;&#x56E0;&#x7D20;&#xFF0C;&#x5305;&#x62EC;&#x5355;&#x5143;&#x683C;&#x5305;&#x88C5;&#x3001; &#x7EC6;&#x80DE;&#x751F;&#x957F;&#x548C;&#x7EC6;&#x80DE;&#x5206;&#x88C2;&#x672C;&#x8EAB;&#x4E4B;&#x95F4;&#x590D;&#x6742;&#x7684;&#x76F8;&#x4E92;&#x4F5C;&#x7528;&#x3002;&#x5728;&#x8FD9;&#x91CC;&#xFF0C;&#x6211;&#x4EEC;&#x4F7F;&#x7528;&#x673A;&#x68B0;&#x6A21;&#x62DF;&#xFF0C;&#x663E;&#x793A;&#x5355;&#x4E2A;&#x5355;&#x5143;&#x683C;&#x7684;&#x591A;&#x8FB9;&#x5F62;&#x5F62;&#x72B6;&#x53EF;&#x4EE5;&#x6709;&#x7CFB;&#x7EDF;&#x5730;&#x504F;&#x5411;&#x4ED6;&#x4EEC;&#x76F8;&#x90BB;&#x7684;&#x6709;&#x4E1D;&#x5206;&#x88C2;&#x90BB;&#x5C45;&#x7684;&#x957F;&#x8F74;&#x65B9;&#x5411;&#x3002;&#x5F15;&#x4EBA;&#x6CE8;&#x76EE;&#x7684;&#x662F;&#xFF0C;&#x52A8;&#x7269;&#x4E0A;&#x76AE;&#x7EC6;&#x80DE;&#x548C;&#x690D;&#x7269;&#x8868;&#x76AE;&#x7684;&#x5206;&#x6790;&#x786E;&#x8BA4;&#x5C40;&#x90E8;&#x7EC6;&#x80DE;&#x62D3;&#x6251;&#x4E0E;&#x4F53;&#x5185;&#x7684;&#x5207;&#x5272;&#x5E73;&#x9762;&#x65B9;&#x5411;&#x9C81;&#x68D2;&#x548C;&#x51E0;&#x4E4E;&#x76F8;&#x540C;&#x7684;&#x76F8;&#x5173;&#x6027;&#x3002;&#x6211;&#x4EEC;&#x4F7F;&#x7528;&#x7B80;&#x5355;&#x7684;&#x6570;&#x5B66;&#xFF0C;&#x663E;&#x793A;&#x6B64;&#x6548;&#x679C;&#x6E90;&#x4E8E;&#x57FA;&#x672C;&#x5305;&#x88C5;&#x7EA6;&#x675F;&#x3002;&#x6211;&#x4EEC;&#x7684;&#x7814;&#x7A76;&#x7ED3;&#x679C;&#x8868;&#x660E;&#x5730;&#x65B9;&#x4E0A;&#x76AE;&#x62D3;&#x6251;&#x662F;&#x5207;&#x5272;&#x5E73;&#x9762;&#x65B9;&#x5411;&#xFF0C;&#x4E00;&#x4E2A;&#x5173;&#x952E;&#x56E0;&#x7D20;&#xFF0C;&#x89E3;&#x7406;&#x9762;&#x504F;&#x89C1;&#x53EF;&#x80FD;&#x662F;&#x666E;&#x904D;&#x7684;&#x690D;&#x7269;&#x548C;&#x52A8;&#x7269;&#x4E2D;&#x7684;&#x591A;&#x8FB9;&#x5F62;&#x5355;&#x5143;&#x683C;&#x5DE5;&#x4F5C;&#x8868;&#x5C5E;&#x6027;&#x3002;

&#x6709;&#x4E1D;&#x5206;&#x88C2;&#x5206;&#x88C2;&#x5E73;&#x9762;&#x7531;&#x5730;&#x65B9;&#x4E0A;&#x76AE;&#x62D3;&#x6251;&#x7684;&#x63A7;&#x5236;&#x3002;

For nearly 150 years, it has been recognized that cell shape strongly influences the orientation of the mitotic cleavage plane (e.g., Hofmeister, 1863). However, we still understand little about the complex interplay between cell shape and cleavage-plane orientation in epithelia, where polygonal cell geometries emerge from multiple factors, including cell packing, cell growth, and cell division itself. Here, using mechanical simulations, we show that the polygonal shapes of individual cells can systematically bias the long-axis orientations of their adjacent mitotic neighbors. Strikingly, analyses of both animal epithelia and plant epidermis confirm a robust and nearly identical correlation between local cell topology and cleavage-plane orientation in vivo. Using simple mathematics, we show that this effect derives from fundamental packing constraints. Our results suggest that local epithelial topology is a key determinant of cleavage-plane orientation, and that cleavage-plane bias may be a widespread property of polygonal cell sheets in plants and animals.

Article	Year
Development (Cambridge, England)\| PubMed ID: 12506005	2003
Science (New York, N.Y.)\| PubMed ID: 19179535	2009
Control of the mitotic cleavage plane by local epithelial topology. Cell\| PubMed ID: 21295702	2011
Development (Cambridge, England)\| PubMed ID: 21389053	2011
Methods (San Diego, Calif.)\| PubMed ID: 21871963	2012

Heather N. Cartwright

Biography

JoVE Journal Publications

Other Publications