in-vitro-impact-model-to-generate-sublethal-chondrocyte-injury-bovine

<em>In Vitro</em> Impact Model to Generate Sublethal Chondrocyte Injury in Bovine Cartilage Explants

Post-Traumatic Osteoarthritis (PTOA) is a prevalent, degenerative, and painful progressive joint disease that typically arises after significant joint ...

Indiana University School of Medicine

Purdue University

The bone marrow microenvironment contains a diverse array of cell types under extensive regulatory control and provides for a novel and complex mechanism for bone regulation. Megakaryocytes (MKs) are one such cell type that potentially acts as a master regulator of the bone marrow microenvironment due to its effects on hematopoiesis, osteoblastogenesis, and osteoclastogenesis. While several of these processes are induced/inhibited through MK secreted factors, others are primarily regulated by direct cell-cell contact. Notably, the regulatory effects that MKs exert on these different cell populations has been found to change with aging and disease states. Overall, MKs are a critical component of the bone marrow that should be considered when examining regulation of the skeletal microenvironment. An increased understanding of the role of MKs in these physiological processes may provide insight into novel therapies that can be used to target specific pathways important in hematopoietic and skeletal disorders.

Megakaryocyte Secreted Factors Regulate Bone Marrow Niche Cells During Skeletal Homeostasis, Aging, and Disease.

This Comment represents three review articles on the relationship between Alzheimer's disease, osteoporosis, and fracture in an exploration of the benefits that AI can provide in scientific writing. The first drafts of the articles were written (1) entirely by humans; (2) entirely by ChatGPT 4.0 (AI-only or AIO); and (3) by humans and ChatGPT 4.0 whereby humans selected literature references, but ChatGPT 4.0 completed the writing (AI-assisted or AIA). Importantly, each review article was edited and carefully checked for accuracy by all co-authors resulting in a final manuscript which was significantly different from the original draft.

Use of AI Language Engine ChatGPT 4.0 to Write a Scientific Review Article Examining the Intersection of Alzheimer's Disease and Bone.

This comprehensive review discusses the complex relationship between Alzheimer's disease (AD) and osteoporosis, two conditions that are prevalent in the aging population and result in adverse complications on quality of life. The purpose of this review is to succinctly elucidate the many commonalities between the two conditions, including shared pathways, inflammatory and oxidative mechanisms, and hormonal deficiencies.

Mind Gaps and Bone Snaps: Exploring the Connection Between Alzheimer's Disease and Osteoporosis.

This review examines the linked pathophysiology of Alzheimer's disease/related dementia (AD/ADRD) and bone disorders like osteoporosis. The emphasis is on "inflammaging"-a low-level inflammation common to both, and its implications in an aging population.

Mind the Gap: Unraveling the Intricate Dance Between Alzheimer's Disease and Related Dementias and Bone Health.

This comprehensive review delves into the intricate interplay between Alzheimer's disease (AD) and osteoporosis, two prevalent conditions with significant implications for individuals' quality of life. The purpose is to explore their bidirectional association, underpinned by common pathological processes such as aging, genetic factors, inflammation, and estrogen deficiency.

From the Mind to the Spine: The Intersecting World of Alzheimer's and Osteoporosis.

Bone marrow (BM) is a tissue that is of great importance to several areas of basic and translational research, including hematology, oncology, bone biology, and immunology. It is unique in that it is gelatinous in nature but housed in a hard casing of bone. Traditionally, flow cytometry and immunofluorescence (IF) techniques have been employed to study the composition of cellular interactions and elements of the BM. However, it has been challenging to study the BM in an unperturbed state using multiple fluorescent probes at a time to fully appreciate the diverse cell populations and their interactions and relative positioning with each other. This protocol addresses how Phenocycler 2.0, which uses co-detection by indexing (CODEX) in conjunction with HALO 4.0 image analysis software, can overcome the obstacles faced by traditional techniques used to study the BM in an unperturbed state.

Article	Year
Megakaryocyte Secreted Factors Regulate Bone Marrow Niche Cells During Skeletal Homeostasis, Aging, and Disease. Calcified tissue international\| PubMed ID: 37243755	2023
Use of AI Language Engine ChatGPT 4.0 to Write a Scientific Review Article Examining the Intersection of Alzheimer's Disease and Bone. Current osteoporosis reports\| PubMed ID: 38225472	2024
Mind Gaps and Bone Snaps: Exploring the Connection Between Alzheimer's Disease and Osteoporosis. Current osteoporosis reports\| PubMed ID: 38236512	2024
Mind the Gap: Unraveling the Intricate Dance Between Alzheimer's Disease and Related Dementias and Bone Health. Current osteoporosis reports\| PubMed ID: 38285083	2024
From the Mind to the Spine: The Intersecting World of Alzheimer's and Osteoporosis. Current osteoporosis reports\| PubMed ID: 38334917	2024
Multiplex imaging of murine bone marrow using Phenocycler 2.0™. Leukemia\| PubMed ID: 40217069	2025

Sonali J. Karnik

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