subcutaneous-injection-human-colorectal-cancer-cells-athymic-nude

Subcutaneous Injection of Human Colorectal Cancer Cells in Athymic Nude Mice to Evaluate Antitumor Efficacy

Colorectal cancer (CRC) is one of the leading causes of cancer-related deaths worldwide, highlighting the urgent need for improved preclinical models to ...

Hospital Clínico Universitario "Virgen de la Arrixaca" and Universidad de Murcia

In airways, a proliferative effect is played directly by cholinergic agonists through nicotinic and muscarinic receptors activation. How tumors respond to aberrantly activated cholinergic signalling is a key question in smoking-related cancer. This research was addressed to explore a possible link of cholinergic signalling changes with cancer biology.

Dysregulated cholinergic network as a novel biomarker of poor prognostic in patients with head and neck squamous cell carcinoma.

Previous reports have demonstrated that a non-neuronal cholinergic system is expressed aberrantly in airways. A proliferative effect is exerted directly by cholinergic agonists through the activation of nicotinic and muscarinic receptors. In cancer, particularly those related with smoking, the mechanism through which tumour cells respond to aberrantly activated cholinergic signalling is a key question. Fifty paired pieces of larynx squamous cell carcinoma and adjacent non-cancerous tissue were compared in terms of their acetylcholinesterase activity (AChE). The AChE activity in non-cancerous tissues (0.248 ± 0.030 milliunits per milligram of wet tissue; mU/mg) demonstrates that upper respiratory tissues express sufficient AChE activity for controlling the level of acetylcholine (ACh). In larynx carcinomas, the AChE activity decreased to 0.157 ± 0.024 mU/mg (p=0.009). Larynx cancer patients exhibiting low ACh-degrading enzymatic activity had a significantly shorter overall survival (p=0.031). Differences in the mRNA levels of alternatively spliced AChE isoforms and molecular compositions were noted between glottic and supraglottic cancers. Our results suggest that the low AChE activity observed in larynx squamous cell carcinoma may be useful for predicting the outcome of patients.

Unbalanced acetylcholinesterase activity in larynx squamous cell carcinoma.

Prognostic value of the Breslow:diameter ratio in cutaneous melanoma.

Today, cell therapies are constantly evolving and providing new options for cancer patients. These therapies are mostly based on the inoculation of immune cells extracted from a person's own tumor; however, some studies using whole tumor cell-based vaccines are approaching the level of maturity required for clinical use. Although these latest therapies will have to be developed further and adapted to overcome many ethical barriers, there is no doubt that therapeutic cancer vaccines are the next frontier of immunotherapy.

Article	Year
Dysregulated cholinergic network as a novel biomarker of poor prognostic in patients with head and neck squamous cell carcinoma. BMC cancer\| PubMed ID: 25956553	2015
Unbalanced acetylcholinesterase activity in larynx squamous cell carcinoma. International immunopharmacology\| PubMed ID: 26002584	2015
Prognostic value of the Breslow:diameter ratio in cutaneous melanoma. Journal of the American Academy of Dermatology\| PubMed ID: 30287323	2019
Ex vivo engineering of phagocytic signals in breast cancer cells for a whole tumor cell-based vaccine. BMC cancer\| PubMed ID: 40596971	2025

Juan Cabezas-Herrera

Biography

JoVE Journal Publications

Other Publications