transmesenteric-laparoscopic-pyeloplasty-trendelenburg-position-for

Transmesenteric Laparoscopic Pyeloplasty in Trendelenburg Position for Horseshoe Kidney with Hydronephrosis

Due to anomalous anatomy, pyeloplasty with a horseshoe kidney (HSK) presents a technical challenge. Previously, we reported a technique of intraperitoneal ...

Shenzhen university

Shenzhen Huazhong University of Science and Technology Research Institute

Management of a long proximal ureteral stricture is challenging. Lingual mucosal graft ureteroplasty (LMGU) is a novel minimally invasive technique for ureteral reconstruction that avoids the morbidity of bowel interposition or autotransplantation.

Lingual Mucosal Graft Ureteroplasty for Long Proximal Ureteral Stricture: 6 Years of Experience with 41 Cases.

About 10-20% of patients with bladder cancer (BC) progress to muscle-invasive diseases, of which the underlying key molecular events have yet to be addressed.

PABPN1 regulates mRNA alternative polyadenylation to inhibit bladder cancer progression.

Alternative polyadenylation (APA) is emerging as a major posttranscriptional mechanism for gene regulation in cancer. A prevailing hypothesis is that shortening of the 3' untranslated region (3'UTR) increases oncoprotein expression because of the loss of miRNA-binding sites (MBSs). We showed that the longer 3'UTR is associated with a more advanced tumor stage in patients with clear cell renal cell carcinoma (ccRCC). More surprisingly, 3'UTR shortening is correlated with better overall survival in patients with ccRCC. Furthermore, we identified a mechanism by which longer transcripts lead to increased oncogenic protein and decreased tumor-suppressive protein expression compared to the shorter transcripts. In our model, shortening of 3'UTRs by APA may increase the mRNA stability of the majority of the potential tumor-suppressor genes due to the loss of MBSs and AU-rich elements (AREs). Unlike potential tumor-suppressor genes, the potential oncogenes display much lower MBS and ARE density and globally much higher m6A density in distal 3'UTRs. As a result, 3'UTRs shortening decreases the mRNA stability of potential oncogenes and enhances the mRNA stability of potential tumor-suppressor genes. Our findings highlight the cancer-specific pattern of APA regulation and extend our understanding of the mechanism of APA-mediated 3'UTR length changes in cancer biology.

Lengthening of 3' Untranslated Regions of mRNAs by Alternative Polyadenylation Is Associated With Tumor Progression and Poor Prognosis of Clear Cell Renal Cell Carcinoma.

Clear cell renal cell carcinoma (ccRCC) is the most common renal cancer. Alternative polyadenylation (APA) plays an important role in the progression and immunity of multiple tumors. Although immunotherapy has emerged as an important treatment option for metastatic renal cell carcinoma, whether APA affects the tumor immune microenvironment (TIME) in ccRCC remains unclear.

Comprehensive analysis of alternative polyadenylation regulators of CTLA4 and immune infiltration in clear cell renal cell carcinoma.

Alternative polyadenylation (APA) is an important post-transcriptional regulatory mechanism in cancer development and progression. Poly(A) binding protein nuclear 1 (PABPN1) is a gene that encodes abundant nuclear protein, binds with high affinity to nascent poly(A) tails, and is crucial for 3'-UTR (3'-untranslated region) APA. Although PABPN1 has been recently reported as a dominant master APA regulator in clear cell renal cell carcinoma (ccRCC), the underlying functional mechanism remain unclear and the genes subject to PABPN1 regulation that contribute to ccRCC progression have not been identified. Here, we found that PABPN1 is upregulated in ccRCC, and its expression is highly associated with the clinical prognosis of ccRCC patients. PABPN1 promotes ccRCC cell proliferation, migration, invasion, and exerts an influence on sphingolipid metabolism and cell cycle. Moreover, PABPN1 depletion significantly suppressed cancer cell growth via induction of cell cycle arrest and apoptosis. In particular, we characterized PABPN1-regulated 3'-UTR APA of sphingosine-1-phosphate lyase 1 (SGPL1) and cellular repressor of E1A stimulated genes 1 (CREG1), which contribute to ccRCC progression. Collectively, our data revealed that PABPN1 promotes ccRCC progression at least in part, by suppressing SGPL1 and CREG1. Thus, PABPN1 may be a potential therapeutic target in ccRCC.

PABPN1 promotes clear cell renal cell carcinoma progression by suppressing the alternative polyadenylation of SGPL1 and CREG1.

The crosstalk between genomic alterations and metabolic dysregulation in bladder cancer is largely unknown. A deep understanding of the interactions between cancer drivers and cancer metabolic changes will provide novel opportunities for targeted therapeutic strategies.

Multi-omics analysis reveals critical metabolic regulators in bladder cancer.

Technique modifications that aim to improve ergonomics of the surgical procedure without repositioning the upper tract urothelial carcinoma patients remain a challenge to urologists. We offer a novel technique to perform intraperitoneal laparoscopic single-site radical nephroureterectomy and pelvic lymph nodes dissection/retroperitoneal lymph nodes dissection in a supine position. Our novel technique is feasible and offers a significant improvement in operative efficiency, particularly in patients with locally advanced disease.

Intraperitoneal laparoscopic single-site lymph node dissection in modified supine position during laparoscopic radical nephroureterectomy.

UHRF1 (Ubiquitin-like with PHD and Ring Finger domains 1) is a crucial E3 ubiquitin ligase and epigenetic regulator with pivotal roles in various biological processes, including the maintenance of DNA methylation, regulation of gene expression, and facilitation of DNA damage repair. In this study, we unveil that UHRF1 interacts with the nonhomologous end joining factor XLF (also known as Cernunnos) following DNA double strand breaks in HeLa cells. Furthermore, we demonstrate that UHRF1 catalyzes lysine 63-linked polyubiquitination of XLF, rather than lysine 48-linked polyubiquitination. Notably, this polyubiquitination of XLF by UHRF1 does not affect its protein stability; instead, it enhances the recruitment of XLF to the sites of DNA damage. These findings shed light on the role of UHRF1 as a novel regulator of DNA repair through XLF in tumor cells.

UHRF1-mediated ubiquitination of nonhomologous end joining factor XLF promotes DNA repair in human tumor cells.

To evaluate the safety and feasibility of intraperitoneal laparoscopic surgery in Trendelenburg position (ILSTP) for pyelolithotomy, pyeloplasty, and heminephrectomy in patients with horseshoe kidneys (HSKs).

Intraperitoneal laparoscopic technique in trendelenburg position: an effective surgical method for pyelolithotomy, pyeloplasty, and heminephrectomy in patients with horseshoe kidneys.

Aggregation-induced emission (AIE) or aggregation-induced emission enhancement (AIEE) has endowed gold species with responsive fluorescent properties, favoring their potential applications in sensing, imaging, and therapy. However, it remains an interesting challenge to fabricate fluorophores with both AIE and AIEE effects. Herein, we presented highly luminescent Au(I) thiolate nanocomplex-based biosensors with Zn induced-AIE and zeolite imidazolate framework (ZIF-8) induced-AIEE effects. The nonemissive monovalent gold-glutathione complexes (Au-SGs) were obtained to synthesize the core-shell Zn/Au-SG@ZIF-8 composites with strong luminescence via the coordination-assisted self-assembly strategy. By immobilizing GOx on the surface of Zn/Au-SG@ZIF-8, Zn/Au-SG@ZIF-8/GOx biosensors exhibited effective responsiveness to glucose, showing a "turn-off" detection model. The mechanism study revealed that the robust luminescence of Zn/Au-SG@ZIF-8 to glucose sensing was attributed to the acid-stimulated degradation of the probe facilitated by H generated from the glucose oxidase (GOx)-catalyzed oxidation process. To achieve noninvasive and intelligent blood glucose detection, the Zn/Au-SG@ZIF-8/GOx-loaded microneedle (MN)-patch fluorescent platform was further developed. The MN-patch-based sensing platform had promising performance for on-needle capture and in situ glucose detection. This study demonstrated a universal and feasible protocol to construct luminescent biosensors for glucose detection and their potential for the development of MN-based analytical devices.

Stepwise Lighting Up Gold(I)-Thiolate Complexes from AIE Nanoaggregates to AIEE Nanoprobes with a ZIF-8 Shell for Glucose Biosensing.

Zinner syndrome (ZS) is a rare anomaly of the Wolffian duct. Here, we present a case of seminal vesiculectomy and ipsilateral nephroureterectomy using laparoscopic surgery in a modified supine position, which was performed in a 30-year-old male patient with ZS. To our knowledge, this is the first report to describe the feasible use of this approach to remove the seminal vesicle and ipsilateral atrophic kidney in a single session.

Article	Year
Lingual Mucosal Graft Ureteroplasty for Long Proximal Ureteral Stricture: 6 Years of Experience with 41 Cases. European urology\| PubMed ID: 35618522	2022
PABPN1 regulates mRNA alternative polyadenylation to inhibit bladder cancer progression. Cell & bioscience\| PubMed ID: 36879298	2023
Lengthening of 3' Untranslated Regions of mRNAs by Alternative Polyadenylation Is Associated With Tumor Progression and Poor Prognosis of Clear Cell Renal Cell Carcinoma. Laboratory investigation; a journal of technical methods and pathology\| PubMed ID: 36889542	2023
Comprehensive analysis of alternative polyadenylation regulators of CTLA4 and immune infiltration in clear cell renal cell carcinoma. Translational andrology and urology\| PubMed ID: 37181233	2023
PABPN1 promotes clear cell renal cell carcinoma progression by suppressing the alternative polyadenylation of SGPL1 and CREG1. Carcinogenesis\| PubMed ID: 37452741	2023
Multi-omics analysis reveals critical metabolic regulators in bladder cancer. International urology and nephrology\| PubMed ID: 37882969	2024
Intraperitoneal laparoscopic single-site lymph node dissection in modified supine position during laparoscopic radical nephroureterectomy. Journal of surgical case reports\| PubMed ID: 38840897	2024
UHRF1-mediated ubiquitination of nonhomologous end joining factor XLF promotes DNA repair in human tumor cells. The Journal of biological chemistry\| PubMed ID: 39341501	2024
Intraperitoneal laparoscopic technique in trendelenburg position: an effective surgical method for pyelolithotomy, pyeloplasty, and heminephrectomy in patients with horseshoe kidneys. BMC urology\| PubMed ID: 39468533	2024
Stepwise Lighting Up Gold(I)-Thiolate Complexes from AIE Nanoaggregates to AIEE Nanoprobes with a ZIF-8 Shell for Glucose Biosensing. Analytical chemistry\| PubMed ID: 39841624	2025
Intraperitoneal Laparoscopic Seminal Vesiculectomy and Ipsilateral Nephroureterectomy in Modified Supine Position for Treatment of Zinner's Syndrome. Asian journal of endoscopic surgery\| PubMed ID: 40302168	2025

Teng Hou

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