Ronald N. Germain

Lymphocyte Biology Section and Center for Advanced Tissue Imaging, NIH

Affiliated withNIH

Research Area

Biography

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JoVE Journal Publications

ArticleTotal : 1	Year
Publication title	2025

Other Publications

Article	Year
Response to 'Class II essential for CD4 survival'. Nature immunology\| PubMed ID: 11175807	2001
T-cell development and the CD4-CD8 lineage decision. Nature reviews. Immunology\| PubMed ID: 12033737	2002
Constitutive presentation of a natural tissue autoantigen exclusively by dendritic cells in the draining lymph node. The Journal of experimental medicine\| PubMed ID: 12391019	2002
T-cell activation: the power of one. Current biology : CB\| PubMed ID: 12593815	2003
Ligand-dependent regulation of T cell development and activation. Immunologic research\| PubMed ID: 12857974	2003
In vivo antigen presentation. Current opinion in immunology\| PubMed ID: 14734120	2004
An innately interesting decade of research in immunology. Nature medicine\| PubMed ID: 15580257	2004
An extended vision for dynamic high-resolution intravital immune imaging. Seminars in immunology\| PubMed ID: 16216522	2005
Decreased CD4 expression by polarized T helper 2 cells contributes to suboptimal TCR-induced phosphorylation and reduced Ca2+ signaling. European journal of immunology\| PubMed ID: 16276482	2005
Extrafollicular activation of lymph node B cells by antigen-bearing dendritic cells. Science (New York, N.Y.)\| PubMed ID: 16778060	2006
Dynamic imaging of the immune system: progress, pitfalls and promise. Nature reviews. Immunology\| PubMed ID: 16799470	2006
Key role of local regulation in chemosensing revealed by a new molecular interaction-based modeling method. PLoS computational biology\| PubMed ID: 16854213	2006
Stromal cell networks regulate lymphocyte entry, migration, and territoriality in lymph nodes. Immunity\| PubMed ID: 17112751	2006
Dynamic imaging of dendritic cell extension into the small bowel lumen in response to epithelial cell TLR engagement. The Journal of experimental medicine\| PubMed ID: 17145958	2006
Highways, byways and breadcrumbs: directing lymphocyte traffic in the lymph node. Trends in immunology\| PubMed ID: 17625969	2007
Special regulatory T-cell review: A rose by any other name: from suppressor T cells to Tregs, approbation to unbridled enthusiasm. Immunology\| PubMed ID: 18154615	2008
Macrophage and T cell dynamics during the development and disintegration of mycobacterial granulomas. Immunity\| PubMed ID: 18261937	2008
Making friends in out-of-the-way places: how cells of the immune system get together and how they conduct their business as revealed by intravital imaging. Immunological reviews\| PubMed ID: 18275481	2008
T cell heterogeneity: firmly fixed, predominantly plastic or merely malleable? Nature immunology\| PubMed ID: 18425095	2008
Fibroblastic reticular cells guide T lymphocyte entry into and migration within the splenic T cell zone. Journal of immunology (Baltimore, Md. : 1950)\| PubMed ID: 18768849	2008
SAP-controlled T-B cell interactions underlie germinal centre formation. Nature\| PubMed ID: 18843362	2008
Sphingosine-1-phosphate mobilizes osteoclast precursors and regulates bone homeostasis. Nature\| PubMed ID: 19204730	2009
House dust mite allergen induces asthma via Toll-like receptor 4 triggering of airway structural cells. Nature medicine\| PubMed ID: 19330007	2009
Computational reconstruction of cell and tissue surfaces for modeling and data analysis. Nature protocols\| PubMed ID: 19536268	2009
Quantifying cellular interaction dynamics in 3D fluorescence microscopy data. Nature protocols\| PubMed ID: 19696749	2009
Visualization and identification of IL-7 producing cells in reporter mice. PloS one\| PubMed ID: 19907640	2009
The in situ dynamics of dendritic cell interactions. European journal of immunology\| PubMed ID: 20853494	2010
Computational analysis of T cell receptor signaling and ligand discrimination--past, present, and future. FEBS letters\| PubMed ID: 20965176	2010
Vaccines and the future of human immunology. Immunity\| PubMed ID: 21029956	2010
Trafficking of a dual-modality magnetic resonance and fluorescence imaging superparamagnetic iron oxide-based nanoprobe to lymph nodes. Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging\| PubMed ID: 21080233	2011
Chemorepulsion by blood S1P regulates osteoclast precursor mobilization and bone remodeling in vivo. The Journal of experimental medicine\| PubMed ID: 21135136	2010
Systems biology in immunology: a computational modeling perspective. Annual review of immunology\| PubMed ID: 21219182	2011
The human condition: an immunological perspective. Nature immunology\| PubMed ID: 21502986	2011
Protective T cell immunity in mice following protein-TLR7/8 agonist-conjugate immunization requires aggregation, type I IFN, and multiple DC subsets. The Journal of clinical investigation\| PubMed ID: 21540549	2011
Uncovering the role of invariant chain in controlling MHC class II antigen capture. Journal of immunology (Baltimore, Md. : 1950)\| PubMed ID: 21772033	2011
Regulatory T cells selectively control CD8+ T cell effector pool size via IL-2 restriction. Journal of immunology (Baltimore, Md. : 1950)\| PubMed ID: 21849683	2011
Baruj Benacerraf (1920-2011). Nature\| PubMed ID: 21886149	2011
Computational modeling of cellular signaling processes embedded into dynamic spatial contexts. Nature methods\| PubMed ID: 22286385	2012
Cancer-associated epithelial cell adhesion molecule (EpCAM; CD326) enables epidermal Langerhans cell motility and migration in vivo. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 22411813	2012
A decade of imaging cellular motility and interaction dynamics in the immune system. Science (New York, N.Y.)\| PubMed ID: 22745423	2012
Histo-cytometry: a method for highly multiplex quantitative tissue imaging analysis applied to dendritic cell subset microanatomy in lymph nodes. Immunity\| PubMed ID: 22863836	2012
CD4 T follicular helper cell dynamics during SIV infection. The Journal of clinical investigation\| PubMed ID: 22922258	2012
A spatially-organized multicellular innate immune response in lymph nodes limits systemic pathogen spread. Cell\| PubMed ID: 22980983	2012
Maintaining system homeostasis: the third law of Newtonian immunology. Nature immunology\| PubMed ID: 22990887	2012
Quantification of lymph node transit times reveals differences in antigen surveillance strategies of naive CD4+ and CD8+ T cells. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 23071319	2012
The calcium-sensing receptor regulates the NLRP3 inflammasome through Ca2+ and cAMP. Nature\| PubMed ID: 23143333	2012
Recent progress using systems biology approaches to better understand molecular mechanisms of immunity. Seminars in immunology\| PubMed ID: 23238271	2013
T cell-positive selection uses self-ligand binding strength to optimize repertoire recognition of foreign antigens. Immunity\| PubMed ID: 23290521	2013
Dynamic visualization of RANKL and Th17-mediated osteoclast function. The Journal of clinical investigation\| PubMed ID: 23321670	2013
Peripheral prepositioning and local CXCL9 chemokine-mediated guidance orchestrate rapid memory CD8+ T cell responses in the lymph node. Immunity\| PubMed ID: 23352234	2013
The adaptor MAVS promotes NLRP3 mitochondrial localization and inflammasome activation. Cell\| PubMed ID: 23582325	2013
Neutrophil swarms require LTB4 and integrins at sites of cell death in vivo. Nature\| PubMed ID: 23708969	2013
A systems analysis identifies a feedforward inflammatory circuit leading to lethal influenza infection. Cell\| PubMed ID: 23827683	2013
Pathogen-related differences in the abundance of presented antigen are reflected in CD4+ T cell dynamic behavior and effector function in the lung. Journal of immunology (Baltimore, Md. : 1950)\| PubMed ID: 24431231	2014
Tuning of antigen sensitivity by T cell receptor-dependent negative feedback controls T cell effector function in inflamed tissues. Immunity\| PubMed ID: 24440150	2014
Open questions: a rose is a rose is a rose--or not? BMC biology\| PubMed ID: 24484903	2014
The multiple faces of leukocyte interstitial migration. Seminars in immunopathology\| PubMed ID: 24573488	2014
Visualization and dynamic analysis of host-pathogen interactions. Current opinion in immunology\| PubMed ID: 24705104	2014
Global analyses of human immune variation reveal baseline predictors of postvaccination responses. Cell\| PubMed ID: 24725414	2014
Focusing in on T cell cross-reactivity. Cell\| PubMed ID: 24855938	2014
T-cell-receptor-dependent signal intensity dominantly controls CD4(+) T cell polarization In Vivo. Immunity\| PubMed ID: 24981853	2014
Revisiting thymic positive selection and the mature T cell repertoire for antigen. Immunity\| PubMed ID: 25148022	2014
DOCK8 regulates lymphocyte shape integrity for skin antiviral immunity. The Journal of experimental medicine\| PubMed ID: 25422492	2014
Strategically localized dendritic cells promote rapid T cell responses to lymph-borne particulate antigens. Immunity\| PubMed ID: 25607462	2015
Network representations of immune system complexity. Wiley interdisciplinary reviews. Systems biology and medicine\| PubMed ID: 25625853	2015
Antigen- and cytokine-driven accumulation of regulatory T cells in visceral adipose tissue of lean mice. Cell metabolism\| PubMed ID: 25863247	2015
Healing the NIH-Funded Biomedical Research Enterprise. Cell\| PubMed ID: 26091028	2015
Targeted Proteomics-Driven Computational Modeling of Macrophage S1P Chemosensing. Molecular & cellular proteomics : MCP\| PubMed ID: 26199343	2015
Robust Anti-viral Immunity Requires Multiple Distinct T Cell-Dendritic Cell Interactions. Cell\| PubMed ID: 26296422	2015
NK-DC crosstalk controls the autopathogenic Th17 response through an innate IFN-γ-IL-27 axis. The Journal of experimental medicine\| PubMed ID: 26347474	2015
Microbiota-Dependent Sequelae of Acute Infection Compromise Tissue-Specific Immunity. Cell\| PubMed ID: 26451485	2015
Immune homeostasis enforced by co-localized effector and regulatory T cells. Nature\| PubMed ID: 26605524	2015
William E. Paul, M.D. (1936-2015), President, The American Association of Immunologists, 1986-1987. Journal of immunology (Baltimore, Md. : 1950)\| PubMed ID: 26637660	2015
Distinct NF-κB and MAPK Activation Thresholds Uncouple Steady-State Microbe Sensing from Anti-pathogen Inflammatory Responses. Cell systems\| PubMed ID: 27237739	2016
Allergen-Induced CD4+ T Cell Cytokine Production within Airway Mucosal Dendritic Cell-T Cell Clusters Drives the Local Recruitment of Myeloid Effector Cells. Journal of immunology (Baltimore, Md. : 1950)\| PubMed ID: 27903737	2017
Adjuvant and carrier protein-dependent T-cell priming promotes a robust antibody response against the Plasmodium falciparum Pfs25 vaccine candidate. Scientific reports\| PubMed ID: 28091576	2017
Follicular CD8 T cells accumulate in HIV infection and can kill infected cells in vitro via bispecific antibodies. Science translational medicine\| PubMed ID: 28100833	2017
CD8 T Cells Orchestrate pDC-XCR1 Dendritic Cell Spatial and Functional Cooperativity to Optimize Priming. Immunity\| PubMed ID: 28190711	2017
A Tunable Diffusion-Consumption Mechanism of Cytokine Propagation Enables Plasticity in Cell-to-Cell Communication in the Immune System. Immunity\| PubMed ID: 28389069	2017
Co-stimulatory function in primary germinal center responses: CD40 and B7 are required on distinct antigen-presenting cells. The Journal of experimental medicine\| PubMed ID: 28768709	2017
Memory-phenotype CD4 T cells spontaneously generated under steady-state conditions exert innate T1-like effector function. Science immunology\| PubMed ID: 28783663	2017
Multiplex, quantitative cellular analysis in large tissue volumes with clearing-enhanced 3D microscopy (C3D). Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 28808033	2017
Migrating Myeloid Cells Sense Temporal Dynamics of Chemoattractant Concentrations. Immunity\| PubMed ID: 29166587	2017
Robust control of the adaptive immune system. Seminars in immunology\| PubMed ID: 29290544	2018
S1P-dependent interorgan trafficking of group 2 innate lymphoid cells supports host defense. Science (New York, N.Y.)\| PubMed ID: 29302015	2018
Spatial distribution and function of T follicular regulatory cells in human lymph nodes. The Journal of experimental medicine\| PubMed ID: 29769249	2018
Hyperactivated PI3Kδ promotes self and commensal reactivity at the expense of optimal humoral immunity. Nature immunology\| PubMed ID: 30127432	2018
AS03-adjuvanted H5N1 vaccine promotes antibody diversity and affinity maturation, NAI titers, cross-clade H5N1 neutralization, but not H1N1 cross-subtype neutralization. NPJ vaccines\| PubMed ID: 30302282	2018
ILC2s - resident lymphocytes pre-adapted to a specific tissue or migratory effectors that adapt to where they move? Current opinion in immunology\| PubMed ID: 30472437	2019
Immune regulation by glucocorticoids can be linked to cell type-dependent transcriptional responses. The Journal of experimental medicine\| PubMed ID: 30674564	2019
Resident Macrophages Cloak Tissue Microlesions to Prevent Neutrophil-Driven Inflammatory Damage. Cell\| PubMed ID: 30955887	2019
High-dimensional cell-level analysis of tissues with Ce3D multiplex volume imaging. Nature protocols\| PubMed ID: 31028373	2019
The Chemoattractant Receptor Ebi2 Drives Intranodal Naive CD4 T Cell Peripheralization to Promote Effective Adaptive Immunity. Immunity\| PubMed ID: 31053504	2019
Cancer prognosis with shallow tumor RNA sequencing. Nature medicine\| PubMed ID: 32042193	2020
The lymph node at a glance - how spatial organization optimizes the immune response. Journal of cell science\| PubMed ID: 32144196	2020
National Cancer Institute Think-Tank Meeting Report on Proteomic Cartography and Biomarkers at the Single-Cell Level: Interrogation of Premalignant Lesions. Journal of proteome research\| PubMed ID: 32163288	2020
Commensal-driven immune zonation of the liver promotes host defence. Nature\| PubMed ID: 33239787	2021
IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 33376221	2020
Efficient Immune Cell Genome Engineering with Enhanced CRISPR Editing Tools. ImmunoHorizons\| PubMed ID: 33622708	2021
A local regulatory T cell feedback circuit maintains immune homeostasis by pruning self-activated T cells. Cell\| PubMed ID: 34157301	2021
Author Correction: Commensal-driven immune zonation of the liver promotes host defence. Nature\| PubMed ID: 34373651	2021
Mesoscale T cell antigen discrimination emerges from intercellular feedback. Trends in immunology\| PubMed ID: 34493455	2021
DNA origami patterning of synthetic T cell receptors reveals spatial control of the sensitivity and kinetics of signal activation. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 34588308	2021
Spatial mapping of protein composition and tissue organization: a primer for multiplexed antibody-based imaging. Nature methods\| PubMed ID: 34811556	2022
Lentivirus-mediated Conditional Gene Expression. Bio-protocol\| PubMed ID: 34859120	2021
Understanding immunity in a tissue-centric context: Combining novel imaging methods and mathematics to extract new insights into function and dysfunction. Immunological reviews\| PubMed ID: 34918351	2022
Intravital and high-content multiplex imaging of the immune system. Trends in cell biology\| PubMed ID: 34920936	2022
IBEX: an iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues. Nature protocols\| PubMed ID: 35022622	2022
Age-related differences in immune dynamics during SARS-CoV-2 infection in rhesus macaques. Life science alliance\| PubMed ID: 35039442	2022
Robust, persistent adaptive immune responses to SARS-CoV-2 in the oropharyngeal lymphoid tissue of children. Research square\| PubMed ID: 35350206	2022
Spatial meta-transcriptomics reveal associations of intratumor bacteria burden with lung cancer cells showing a distinct oncogenic signature. Journal for immunotherapy of cancer\| PubMed ID: 35793869	2022
Absolute protein quantitation of the mouse macrophage Toll-like receptor and chemotaxis pathways. Scientific data\| PubMed ID: 35961990	2022
Author Correction: Commensal-driven immune zonation of the liver promotes host defence. Nature\| PubMed ID: 36280745	2022
Double knockin mice show NF-κB trajectories in immune signaling and aging. Cell reports\| PubMed ID: 36417863	2022
Adaptive immune responses to SARS-CoV-2 persist in the pharyngeal lymphoid tissue of children. Nature immunology\| PubMed ID: 36536106	2023
Modeling collective cell behavior in cancer: Perspectives from an interdisciplinary conversation. Cell systems\| PubMed ID: 37080161	2023
Spatiotemporal and cell-state control of antigen presentation during tolerance and immunity. Current opinion in immunology\| PubMed ID: 37331219	2023
Organ Mapping Antibody Panels: a community resource for standardized multiplexed tissue imaging. Nature methods\| PubMed ID: 37468619	2023
Dynamic Multiplex Tissue Imaging in Inflammation Research. Annual review of pathology\| PubMed ID: 37722698	2024
The β-adrenergic receptor links sympathetic nerves to T cell exhaustion. Nature\| PubMed ID: 37731001	2023
TCR ligand potency differentially impacts PD-1 inhibitory effects on diverse signaling pathways. The Journal of experimental medicine\| PubMed ID: 37796477	2023
Multiplex imaging in immuno-oncology. Journal for immunotherapy of cancer\| PubMed ID: 37899132	2023
A spatial human thymus cell atlas mapped to a continuous tissue axis. bioRxiv : the preprint server for biology\| PubMed ID: 37986877	2023
Spatial Patterning Analysis of Cellular Ensembles (SPACE) discovers complex spatial organization at the cell and tissue levels. bioRxiv : the preprint server for biology\| PubMed ID: 38168288	2024
Multi-omic profiling of follicular lymphoma reveals changes in tissue architecture and enhanced stromal remodeling in high-risk patients. Cancer cell\| PubMed ID: 38428410	2024
Acute and persistent responses after H5N1 vaccination in humans. Cell reports\| PubMed ID: 39235945	2024
Linking signal input, cell state, and spatial context to inflammatory responses. Current opinion in immunology\| PubMed ID: 39265520	2024
Germline mutations in a G protein identify signaling cross-talk in T cells. Science (New York, N.Y.)\| PubMed ID: 39298586	2024
The IBEX Knowledge-Base: Achieving more together with open science. ArXiv\| PubMed ID: 39398203	2024
A spatial human thymus cell atlas mapped to a continuous tissue axis. Nature\| PubMed ID: 39567784	2024
System vaccinology analysis of predictors and mechanisms of antibody response durability to multiple vaccines in humans. Nature immunology\| PubMed ID: 39747435	2025
The IBEX Imaging Knowledge-Base: A Community Resource Enabling Adoption and Development of Immunofluoresence Imaging Methods. ArXiv\| PubMed ID: 39764400	2024
Spatial Patterning Analysis of Cellular Ensembles (SPACE) finds complex spatial organization at the cell and tissue levels. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 39903116	2025
The IBEX Knowledge-Base: A central resource for multiplexed imaging techniques. PLoS biology\| PubMed ID: 40106489	2025
Robust differentiation of NK cells from MSLN.CAR-IL-15-engineered human iPSCs with enhanced antitumor efficacy against solid tumors. Science advances\| PubMed ID: 40315330	2025