a-structured-approach-to-extubation-in-mechanically-ventilated-rats

A Structured Approach to Extubation in Mechanically Ventilated Rats

Mechanical ventilation (MV) is widely used in critical care but is associated with various complications that affect both short- and long-term outcomes, ...

postoperative-recovery-stability-chronic-intracranial-multielectrode

Postoperative Recovery and Stability of Chronic Intracranial Multielectrode Electroencephalography Recording in Rats

Intracranial multi-electrode implantation enables simultaneous acquisition of neural signals across brain regions but introduces post-surgical ...

Capital Medical University

Postmenopausal osteoporosis caused by estrogen deficiency affects millions of women worldwide. By influencing both osteoblast and osteoclast development, NOD-like receptor thermoprotein structural domain-associated protein 3 (NLRP3) is a key player in the etiology of osteoporosis (OP). The purpose of this research was to look into the mechanism of action of NLRP3 in osteoporosis caused by a lack of estrogen, highlighting that NLRP3 induces osteoblast pyroptosis and thus inflammatory responses in de-ovulated mice, thereby inhibiting osteogenic differentiation and participating in the development of osteoporosis. In de-ovulated mice, we found an enhanced inflammatory response and suppression of osteogenic activity. In vitro experiments, we found a significant increase in markers of cell pyroptosis and inflammatory responses and a significant decrease in markers of osteogenic differentiation in osteoblasts from de-ovulated mice. However, knockdown of the NLRP3 gene inhibited this cell pyroptosis and improved osteogenic differentiation of osteoblasts. Our findings indicate a potential therapeutic potential for the treatment of estrogen deficiency-induced osteoporosis by demonstrating the critical role that NLRP3 inflammatory vesicles and their downstream-mediated cellular pyroptosis play in bone differentiation.

Study on the mechanism of NLRP3 effect on the skeleton of de-ovalized mice.

Phrenic nerve stimulation (PNS) may preserve diaphragm activation and mitigate multiorgan injury during mechanical ventilation (MV); however, a minimal invasive rat model integrating PNS with MV is lacking. We established an omohyoid muscle-based PNS rat model combined with MV. Bilateral nerves were exposed within 20 ± 2 min by transection at the intermediate tendon of omohyoid muscle, minimizing trauma and bleeding. Threshold stimulation (0.6 ± 0.2 mA) correlated with body weight. Ventilator-synchronized stimulation increased compound muscle action potentials by ~30%, whereas histology confirmed intact nerve. Physiological parameters remained stable throughout ventilation. This model provides a safe and scalable platform for mechanistic and preclinical studies on PNS-mediated protection against MV-induced organ injury.

ArticleTotal : 2	Year
Publication title	2025
Publication title	2026

Article	Year
Study on the mechanism of NLRP3 effect on the skeleton of de-ovalized mice. Biochemistry and biophysics reports\| PubMed ID: 37332667	2023
A preclinical rat model for bilateral phrenic nerve stimulation during mechanical ventilation. Animal models and experimental medicine\| PubMed ID: 41760590	2026

Zhize Gao

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