microdroplet-digital-polymerase-chain-reaction-for-precision

Microdroplet Digital Polymerase Chain Reaction for Precision Detection of Non-Small Cell Lung Cancer Mutations

On a global scale, lung cancer continues to be the primary contributor to cancer-related deaths, with its prevalence ranking second only to that of kidney ...

First Affiliated Hospital of Kunming Medical University

miRNAs have been shown to play pivotal roles in the establishment and progression of colon cancer, but their underlying mechanisms are not fully understood. N-acetyltransferase NAA10 participates in many cellular processes, including tumorigenesis. Here we showed that miR-342-5p and miR-608 suppressed the tumorigenesis of colon cancer cells in vitro and in vivo by targeting NAA10 mRNA for degradation. Overexpression of miR-342-5p or miR-608 decreased NAA10 mRNA and protein levels and thereby suppressed cell proliferation, migration, and cell-cycle progression, as well as promoted apoptosis in SW480 and SW620 cells. More importantly, miR-342-5p and miR-608 significantly decreased the tumorigenic capacity of SW480 and SW620 cells in a mouse xenograft model. We also observed an inverse correlation between the expression of NAA10 and that of both miRNAs. Our results implicate miR-342-5p and miR-608 in colon cancer development and unveil the underlying mechanism of this phenomenon, which involves NAA10.

microRNA-342-5p and miR-608 inhibit colon cancer tumorigenesis by targeting NAA10.

Hepatoid adenocarcinoma (HAC) occurs in extrahepatic organs such as the gastrointestinal tract, testes, ovaries, lungs, mediastinum and pancreas, and frequently produces α-fetoprotein (AFP). HAC of the lung (HAL) is rare, characterized by difficult treatment and poor prognosis. There are no reports of HAL in Yunnan-Guizhou Plateau, China.

Primary hepatoid adenocarcinoma of the lung in Yungui Plateau, China: A case report.

The molecular mechanisms involved in small-cell lung cancer (SCLC) are largely unknown. Recent studies have suggested that long non-coding RNAs (lncRNAs) are likely to play a critical role.

Identification of alternative splicing and lncRNA genes in pathogenesis of small cell lung cancer based on their RNA sequencing.

Migration of two clavicular Kirschner wires in different paths at different time: A case report.

To investigate the expression of KRT80 in non-small cell lung cancer (NSCLC) patients and its relationship with clinicopathological factors, prognosis, as well as the differences in KRT80 expression between Xuanwei area and Non-xuanwei area, lung adenocarcinoma and lung squamous cell carcinoma. Based on 178 clinical cases, immunohistochemical methods combined with public databases were used to examine the expression of KRT80 in NSCLC patients and analyze its correlation with various clinicopathological factors and prognosis. The study confirmed the up-regulation of KRT80 expression in NSCLC cancer tissues. Significant regional specific and histological subtype differences in KRT80 expression were revealed. Although the expression level of KRT80 has no direct significant correlation with the prognosis of NSCLC patients, it has a good ability to distinguish cancerous tissue from paracancer tissue. KRT80 can be used as a diagnostic biomarker for NSCLC patients, but its specific mechanism and clinical application value still need to be further explored.

Article	Year
microRNA-342-5p and miR-608 inhibit colon cancer tumorigenesis by targeting NAA10. Oncotarget\| PubMed ID: 26646451	2016
Primary hepatoid adenocarcinoma of the lung in Yungui Plateau, China: A case report. World journal of clinical cases\| PubMed ID: 31367631	2019
Identification of alternative splicing and lncRNA genes in pathogenesis of small cell lung cancer based on their RNA sequencing. Advances in clinical and experimental medicine : official organ Wroclaw Medical University\| PubMed ID: 31403267	2019
Migration of two clavicular Kirschner wires in different paths at different time: A case report. Asian journal of surgery\| PubMed ID: 38845315	2024
Keratin 80 as a diagnostic biomarker with limited prognostic value in non-small cell lung cancer. Scientific reports\| PubMed ID: 40467749	2025

Qingmei Yang

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