the-modified-single-working-portal-technique-using-lasso-loop-stitch

The Modified Single-working Portal Technique Using Lasso-loop Stitch with Needle for Arthroscopic Subscapularis Repair

Traditional shoulder arthroscopy typically requires both anterior and posterior portals. However, in the traditional anterior portal, simultaneous ...

The First Affiliated Hospital of Guangzhou Medical University

Rotator cuff tear (RCT) is a common tendon injury, but the mechanisms of tendon healing remain incompletely understood. Elucidating the molecular mechanisms of tenogenic differentiation is essential to develop novel therapeutic strategies in clinical treatment of RCT. The long noncoding RNA H19 plays a regulatory role in tenogenic differentiation and tendon healing, but its detailed mechanism of action remains unknown. To elucidate the role of H19 in tenogenic differentiation and tendon healing, tendon-derived stem cells were harvested from the Achilles tendons of Sprague Dawley rats and a rat model of cuff tear was established for the exploration of the function of H19 in promoting tenogenic differentiation. The results showed that H19 overexpression promoted, while H19 silencing suppressed, tenogenic differentiation of tendon-derived stem cells (TDSCs). Furthermore, bioinformatic analyses and a luciferase reporter gene assay showed that H19 directly targeted and inhibited miR-140-5p to promote tenogenic differentiation. Further, inhibiting miR-140-5p directly increased VEGFA expression, revealing a novel regulatory axis between H19, miR-140-5p, and VEGFA in modulating tenogenic differentiation. In rats with RTC, implantation of H19-overexpressing TDSCs at the lesion promoted tendon healing and functional recovery. In general, the data suggest that H19 promotes tenogenic differentiation and tendon-bone healing by targeting miR-140-5p and increasing VEGFA levels. Modulation of the H19/miR-140-5p/VEGFA axis in TDSCs is a new potential strategy for clinical treatment of tendon injury.

Long noncoding RNA H19 accelerates tenogenic differentiation by modulating miR-140-5p/VEGFA signaling.

Compared with traditional tendon repair teaching methods, using a virtual reality (VR) simulator to teach tendon suturing can significantly improve medical students' exercise time, operation flow and operation knowledge. At present, the purpose of this study is to explore the long-term influence of VR simulator teaching on the practice performance of medical students.

Researching the application of virtual reality in medical education: one-year follow-up of a randomized trial.

To compare the clinical efficacies of arthroscopic anterior talofibular ligament suture augmentation repair and modified suture augmentation repair in patients with chronic ankle instability (CAI).

Clinical outcomes of arthroscopic all-inside anterior talofibular ligament suture augmentation repair versus modified suture augmentation repair for chronic ankle instability patients.

Prior studies have reported inconsistent results regarding the association between driving pressure-guided ventilation and postoperative pulmonary complications (PPCs). We aimed to investigate whether driving pressure-guided ventilation is associated with a lower risk of PPCs.

Association between driving pressure-guided ventilation and postoperative pulmonary complications in surgical patients: a meta-analysis with trial sequential analysis.

Osteosarcoma (OS), the most common primary malignant bone tumor, predominantly affects children and young adults and is characterized by high invasiveness and poor prognosis. Despite therapeutic advancements, the survival rate remains suboptimal, indicating an urgent need for novel biomarkers and therapeutic targets. This study aimed to investigate the prognostic significance of LGMN expression and immune cell infiltration in the tumor microenvironment of OS.

Investigation of prognostic values of immune infiltration and LGMN expression in the microenvironment of osteosarcoma.

Chronic ankle instability (CAI) is a common and highly disabling condition. Although several studies have evaluated and analyzed prevention and treatment strategies for CAI, an unbiased and systematic synthesis of evidence is required to provide the most powerful and comprehensive evidence-based measures for the its prevention and treatment of CAI. This study aimed to synthesize evidence from the existing literature addressing the treatment and prevention of CAI.

Treatment and prevention of chronic ankle instability: An umbrella review of meta-analyses.

Polycyclic aromatic hydrocarbons (PAHs), especially naphthalene (Nap), pose a significant threat to coastal ecosystems and may contribute to the occurrence of harmful algal blooms (HABs). However, the response mechanisms of HAB species to PAH pollution remain unclear. This study investigated the physiological and molecular responses of the HAB species Heterosigma akashiwo to varying Nap concentrations. Low concentrations (1-10 μg/L) promoted growth, while high concentrations (20-1000 μg/L) inhibited growth, causing abnormal cell morphology and oxidative stress. Within 96 h, H. akashiwo removed 82.38 % (10 μg/L) and 88.93 % (100 μg/L) of Nap, mainly through biodegradation and intracellular accumulation. Transcriptomic analysis revealed that H. akashiwo employs a multifaceted adaptive strategy to cope with Nap stress. Key mechanisms include the upregulation of calcium signaling, transcription factors (e.g., zinc finger protein C2H2, myeloblastosis transcription factor, basic leucine zipper transcription factor), heat shock proteins (e.g., HSP40), and fatty acid desaturase (FAD), enhancing stress tolerance through detoxification, antioxidant responses, and membrane integrity. Activation of peroxisomal and oxidative phosphorylation genes indicates improved detoxification and energy metabolism. Differential expression of genes in the PI3K-Akt pathway reveals dose-dependent growth responses, with low concentrations promoting proliferation and high concentrations inhibiting growth. These findings offer insights into H. akashiwo's adaptive responses to Nap, aiding ecological risk assessment and bioremediation strategies for PAH pollution in marine environments.

Article	Year
Long noncoding RNA H19 accelerates tenogenic differentiation by modulating miR-140-5p/VEGFA signaling. European journal of histochemistry : EJH\| PubMed ID: 34494412	2021
Researching the application of virtual reality in medical education: one-year follow-up of a randomized trial. BMC medical education\| PubMed ID: 36597093	2023
Clinical outcomes of arthroscopic all-inside anterior talofibular ligament suture augmentation repair versus modified suture augmentation repair for chronic ankle instability patients. BMC musculoskeletal disorders\| PubMed ID: 38200504	2024
Association between driving pressure-guided ventilation and postoperative pulmonary complications in surgical patients: a meta-analysis with trial sequential analysis. British journal of anaesthesia\| PubMed ID: 38937217	2024
Investigation of prognostic values of immune infiltration and LGMN expression in the microenvironment of osteosarcoma. Discover oncology\| PubMed ID: 38980440	2024
Treatment and prevention of chronic ankle instability: An umbrella review of meta-analyses. Foot and ankle surgery : official journal of the European Society of Foot and Ankle Surgeons\| PubMed ID: 39107216	2025
Physiological responses and adaptive mechanisms of the harmful algal bloom species Heterosigma akashiwo to naphthalene exposure. Journal of hazardous materials\| PubMed ID: 40056521	2025

Xiaofei Zheng

Biography

JoVE Journal Publications

Other Publications