a-safe-effective-economical-protocol-for-rabbit-vertebral-screw

A Safe, Effective, and Economical Protocol for Rabbit Vertebral Screw Insertion

Posterior lumbar screw fixation is the most common surgical method for lumbar disc herniation, but patients often face multiple complications ...

Tianjin Medical University

The cardiovascular system adaptation occurs during pregnancy to ensure adequate maternal circulation. Progesterone (P4) is widely used in hormone therapy to support pregnancy, but little is known about its effects on maternal cardiac function. In this study, we investigated the cardiac repolarization and ion channel expression in pregnant subjects and mice models and studied the effects of P4 administrations on these pregnancy-mediated adaptations. P4 administrations shortened the prolongation of QTC intervals and action potential duration (APD) that occurred during pregnancy, which was mainly attributable to the reduction in the voltage-gated potassium (Kv) current under basal conditions.  studies indicated that P4 regulated the Kv2.1 channel in a bidirectional manner. At a low dose (1 M), P4 induced upregulation of Kv2.1 through P4 receptor, while at a higher dose (5 M), P4 downregulated Kv2.1 by targeting microRNA-29b (miR-29b). Our data showed that P4 modulated maternal cardiac repolarization by regulating Kv2.1 channel activity during pregnancy. Kv2.1, as well as miR-29b, might be used as potential therapeutic targets for adaptations of the maternal cardiovascular system or evaluation of progesterone medication during pregnancy.

Progesterone Changes the Pregnancy-Induced Adaptation of Cardiomyocyte Kv2.1 Channels via MicroRNA-29b.

Morroniside (MOR) possesses antiosteoporosis (OP) effects, but its molecular target and relevant mechanisms remain unknown.

Morroniside ameliorates glucocorticoid-induced osteoporosis and promotes osteoblastogenesis by interacting with sodium-glucose cotransporter 2.

N4-acetylcytidine (ac4C) is a post-transcriptional RNA modification catalyzed by N-acetyltransferase 10 (NAT10), a critical factor known to influence mRNA stability. However, the role of ac4C in visual development remains unexplored.

Deficiency of Acetyltransferase nat10 in Zebrafish Causes Developmental Defects in the Visual Function.

Statins, widely prescribed for cholesterol management by inhibiting HMG-CoA reductase in the cholesterol biosynthesis pathway, may also influence vertebrate development. In this study, we investigated the developmental effects of two widely used statins, atorvastatin (ATO) and pravastatin (PRA), on zebrafish offspring. For ATO, we administered doses classified as low (1 μM), medium (5 μM), and high (10 μM), while for PRA, the corresponding concentrations were set at low (18 μM), medium (180 μM), and high (270 μM). Our results showed significant reductions in birth and hatching rates, along with decreased body length in offspring at all ATO concentrations and medium to high PRA concentrations. A notable increase in malformation rates, especially in the spine and heart, was observed across all ATO treatments and in medium and high PRA groups. Additionally, we observed reduced heart contraction rates, decreased heart size, lower bone volumes, and diminished expression of mRNA osteogenic markers. Elevated venous sinus-artery bulb (SV-BA) ratios, increased thoracic area, and abnormal cartilage development were also prominent in all ATO-treated groups. Transcriptome analysis revealed alterations in genes predominantly associated with ion channels. These findings provide insights into the potential impacts of specific concentrations of statins on offspring development and highlight potential gene interactions with statins.

Early statin exposure influences cardiac and skeletal development with implications for ion channel transcriptomes in zebrafish.

Osteoporosis, a prevalent systemic bone metabolic disorder, primarily affects postmenopausal women and is characterized by increased bone fragility and a heightened risk of fractures. The efficacy of current osteoporosis treatments is often limited by non-specific drug targeting and undesirable off-target skeletal side effects. To address this challenge, we have developed a novel hydroxyapatite-responsive drug delivery system. This system utilizes a self-assembled p-phosphonatocalix[4]arene tetradodecyl ether (PC4A12C), engineered to specifically target and sustain the release of osteoporosis medication at sites of bone remodeling. Our focus centers on icariin (ICA), a drug known for its potent osteogenic properties and minimal adverse effects. In vitro, ICA-loaded PC4A12C (ICA@PC4A12C) demonstrated enhanced proliferation, differentiation, and mineralization in bone marrow mesenchymal stem cells (BMSCs). In vivo, ICA@PC4A12C exhibited superior efficacy in specifically targeting bone tissue, ensuring a controlled and slow release of icariin directly within the bone environment. In an osteoporosis mouse model, treatment with ICA@PC4A12C showed notable enhancement in osteogenic activity and a significant increase in bone density compared to ICA alone. These results demonstrate the potential of PC4A12C as an effective drug carrier in the development of advanced antiosteoporotic drug delivery systems.

Enhancing osteoporosis treatment using a targeted, sustained-release drug delivery system based on macrocyclic amphiphile.

Bone fractures are frequently encountered in clinical practice and pose significant challenges, due to the high incidence of delayed healing or non-union. Reduced graphene oxide (rGO) stands out for its unique sensitivity to a wide range of light wavelengths, especially in the near-infrared (NIR) spectrum. In this study, we developed an approach to promote fracture healing using an rGO-HAMC hydrogel activated by NIR stimulation. In vitro experiments demonstrated that NIR-stimulated rGO hydrogel significantly enhanced osteogenesis in bone marrow stromal cells (BMSCs). In an in vivo mouse fracture model, the rGO hydrogel was applied to the fracture site, and NIR light was delivered transdermally, showing promising results in accelerating bone regeneration. These results demonstrate that NIR-stimulated rGO hydrogel significantly accelerates fracture healing, as evidenced by increased bone volume and trabecular thickness. Additionally, transcriptomic analysis revealed that pathways related to B-cell activation are implicated in the enhanced healing effects mediated by NIR-stimulated rGO hydrogel. These findings highlight the potential of the NIR-stimulated rGO hydrogel as an innovative strategy for bone regeneration, offering promising new avenues for the treatment and management of bone fractures.

Article	Year
Progesterone Changes the Pregnancy-Induced Adaptation of Cardiomyocyte Kv2.1 Channels via MicroRNA-29b. Cardiovascular therapeutics\| PubMed ID: 35474714	2022
Morroniside ameliorates glucocorticoid-induced osteoporosis and promotes osteoblastogenesis by interacting with sodium-glucose cotransporter 2. Pharmaceutical biology\| PubMed ID: 36786302	2023
Deficiency of Acetyltransferase nat10 in Zebrafish Causes Developmental Defects in the Visual Function. Investigative ophthalmology & visual science\| PubMed ID: 38381411	2024
Early statin exposure influences cardiac and skeletal development with implications for ion channel transcriptomes in zebrafish. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP\| PubMed ID: 38522713	2024
Enhancing osteoporosis treatment using a targeted, sustained-release drug delivery system based on macrocyclic amphiphile. International journal of pharmaceutics\| PubMed ID: 38992736	2024
NIR-stimulated rGO-HAMC hydrogel enhances fracture healing through regulating B-cell signaling. Biomaterials advances\| PubMed ID: 39490190	2025

Yang Hou-zhi

Biography

JoVE Journal Publications

Other Publications