a-minimally-invasive-method-for-generating-syngeneic-orthotopic-mouse

A Minimally Invasive Method for Generating a Syngeneic Orthotopic Mouse Model of Lung Cancer

Non-small cell lung cancer (NSCLC) continues to be the number one cause of cancer-related death for both women and men worldwide. More information needs ...

University of Iowa

Geminii, Inc.

The purpose of this investigation was to identify a quantitative dose-response relationship for enhancing maximal oxygen consumption (VO2max) in healthy sedentary older adults after controlled endurance training.

Dose-response relationship of cardiorespiratory fitness adaptation to controlled endurance training in sedentary older adults.

Aberrant redox signaling underlies the pathophysiology of many chronic metabolic diseases, including type 2 diabetes (T2D). Methodologies aimed at rebalancing systemic redox homeostasis have had limited success. A noninvasive, sustained approach would enable the long-term control of redox signaling for the treatment of T2D. We report that static magnetic and electric fields (sBE) noninvasively modulate the systemic GSH-to-GSSG redox couple to promote a healthier systemic redox environment that is reducing. Strikingly, when applied to mouse models of T2D, sBE rapidly ameliorates insulin resistance and glucose intolerance in as few as 3 days with no observed adverse effects. Scavenging paramagnetic byproducts of oxygen metabolism with SOD2 in hepatic mitochondria fully abolishes these insulin sensitizing effects, demonstrating that mitochondrial superoxide mediates induction of these therapeutic changes. Our findings introduce a remarkable redox-modulating phenomenon that exploits endogenous electromagneto-receptive mechanisms for the noninvasive treatment of T2D, and potentially other redox-related diseases.

Exposure to Static Magnetic and Electric Fields Treats Type 2 Diabetes.

The art of virtual mentoring in the twenty-first century for STEM majors and beyond.

Scientific success is mainly supported by mentoring, which often occurs through face-to-face interactions. Changes to the research environment incurred by the Coronavirus 2019 (COVID-19) pandemic have necessitated mentorship adaptations. Here, we describe how mentors can broaden their mentorship to support trainee growth and provide reassurance about trainee development amid uncertain circumstances.

Mentoring during Uncertain Times.

Counterpoint: An alternative hypothesis for why exposure to static magnetic and electric fields treats type 2 diabetes.

Reply to Petersen et al.: An alternative hypothesis for why exposure to static magnetic and electric fields treats type 2 diabetes.

Primary cilia are microtubule-based organelles present on most cells that regulate many physiological processes, ranging from maintaining energy homeostasis to renal function. However, the role of these structures in the regulation of behavior remains unknown. To study the role of cilia in behavior, we employ mouse models of the human ciliopathy, Bardet-Biedl Syndrome (BBS). Here, we demonstrate that BBS mice have significant impairments in context fear conditioning, a form of associative learning. Moreover, we show that postnatal deletion of BBS gene function, as well as congenital deletion, specifically in the forebrain, impairs context fear conditioning. Analyses indicated that these behavioral impairments are not the result of impaired hippocampal long-term potentiation. However, our results indicate that these behavioral impairments are the result of impaired hippocampal neurogenesis. Two-week treatment with lithium chloride partially restores the proliferation of hippocampal neurons which leads to a rescue of context fear conditioning. Overall, our results identify a novel role of cilia genes in hippocampal neurogenesis and long-term context fear conditioning.

A mouse model of Bardet-Biedl Syndrome has impaired fear memory, which is rescued by lithium treatment.

The rise of novel, more infectious SARS-CoV-2 variants has made clear the need to rapidly deploy large-scale testing for COVID-19 to protect public health. However, testing remains limited due to shortages of personal protective equipment (PPE), naso- and oropharyngeal swabs, and healthcare workers. Simple test methods are needed to enhance COVID-19 screening. Here, we describe a simple, and inexpensive spit-test for COVID-19 screening called Patient Self-Collection of Sample-CoV2 ().

Article	Year
Dose-response relationship of cardiorespiratory fitness adaptation to controlled endurance training in sedentary older adults. European journal of preventive cardiology\| PubMed ID: 25901000	2016
Exposure to Static Magnetic and Electric Fields Treats Type 2 Diabetes. Cell metabolism\| PubMed ID: 33027675	2020
The art of virtual mentoring in the twenty-first century for STEM majors and beyond. Nature biotechnology\| PubMed ID: 33273732	2020
Mentoring during Uncertain Times. Trends in biochemical sciences\| PubMed ID: 33622580	2021
Counterpoint: An alternative hypothesis for why exposure to static magnetic and electric fields treats type 2 diabetes. American journal of physiology. Endocrinology and metabolism\| PubMed ID: 33843282	2021
Reply to Petersen et al.: An alternative hypothesis for why exposure to static magnetic and electric fields treats type 2 diabetes. American journal of physiology. Endocrinology and metabolism\| PubMed ID: 33843283	2021
A mouse model of Bardet-Biedl Syndrome has impaired fear memory, which is rescued by lithium treatment. PLoS genetics\| PubMed ID: 33886537	2021
An open source and convenient method for the wide-spread testing of COVID-19 using deep throat sputum samples. PeerJ\| PubMed ID: 35573180	2022

Sunny C. Huang

Biography

JoVE Journal Publications

Other Publications