network-pharmacological-analysis-tcm-formulae-based-on-syndrome

Network Pharmacological Analysis of TCM Formulae Based on the Syndrome Ontology and Multidimensional Quantitative Association Computing Platform

Current network pharmacology methods for TCM formulae predict potential active ingredients and mechanisms of action for specific diseases by constructing ...

Sichuan University

Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education

Combining fluoride and antimicrobial agents enhances regulation of acid and exopolysaccharide production by biofilms. The combination also weakens the acidogenic and aciduric bacteria that contribute to caries, achieving stronger caries-controlling effects with lower concentrations of fluoride. In previous studies, antimicrobial peptide GH12 has been shown to inhibit lactic acid and exopolysaccharide synthesis in various cariogenic biofilm models, and reduce the proportion of acidogenic bacteria and Keyes caries scores in a rat caries model. The current study aimed to elucidate the effect of a combination of low concentrations of sodium fluoride (NaF) and GH12 and to determine the mechanism by which GH12/NaF combination controls caries. The GH12/NaF combination contained 8 mg/L GH12 and 250 ppm NaF. A rat caries model was built, and rat dental plaque was sampled and cultivated on bovine enamel slabs in vitro and subjected to short-term treatment (5 min, 3 times/day). The caries-controlling effects were evaluated using Keyes scoring and transverse microradiography. The results showed that the GH12/NaF combination significantly decreased the onset and development of dental caries, as well as mineral content loss and lesion depth in vitro (p < 0.05). For the caries-controlling mechanisms, 16S rRNA sequencing of in vivo dental plaque revealed that populations of commensal bacteria Rothia spp. and Streptococcus parasanguinis increased in the GH12/NaF group. In contrast, Veillonella, Lactobacillus, and Streptococcus mutans decreased. Furthermore, the GH12/NaF combination significantly reduced biomass, lactic acid, and exopolysaccharides production of in vitro biofilm (p < 0.05). Overall, fluoride and GH12 efficiently arrested caries development and demineralization by regulating the microbiota and suppressing acid and exopolysaccharide production in biofilms.

Combined Treatment with Fluoride and Antimicrobial Peptide GH12 Efficiently Controls Caries in vitro and in vivo.

An accurate assessment of the surgical margins of tongue squamous cell carcinoma (TSCC), especially the deep muscle tissue, can help completely remove the cancer cells and thus minimize the risk of recurrence. This study aimed to develop a classification model that classifies TSCC and normal tissues in order to aid in the rapid and accurate intraoperative assessment of TSCC surgical deep muscle tissue margins.

Intraoperative rapid assessment of the deep muscle surgical margin of tongue squamous cell carcinoma via Raman spectroscopy.

This study introduces a novel classification approach that combines convolutional neural network (CNN) and Raman mapping to differentiate between tongue squamous cell carcinoma (TSCC) and non-tumorous tissue, as well as to identify different histological grades of TSCC.

Assessment of Surgical Margin of Tongue Squamous Cell Carcinoma via Raman Mapping.

To establish a precise and efficient diagnostic framework for distinguishing medication-related osteonecrosis of the jaw, radiation-induced osteonecrosis of the jaw, and normal bone tissue, thus enhancing clinical decision-making and enabling targeted therapeutic interventions.

Article	Year
Combined Treatment with Fluoride and Antimicrobial Peptide GH12 Efficiently Controls Caries in vitro and in vivo. Caries research\| PubMed ID: 36380626	2022
Intraoperative rapid assessment of the deep muscle surgical margin of tongue squamous cell carcinoma via Raman spectroscopy. Frontiers in bioengineering and biotechnology\| PubMed ID: 39439553	2024
Assessment of Surgical Margin of Tongue Squamous Cell Carcinoma via Raman Mapping. Oral diseases\| PubMed ID: 39815649	2025
Label-free rapid diagnosis of jaw osteonecrosis via the intersection of Raman spectroscopy and deep learning. Bone\| PubMed ID: 40320103	2025

Li Zhongxu

Biography

JoVE Journal Publications

Other Publications