analysis-influence-clinical-nursing-pathway-construction

Analysis of Influence of Clinical Nursing Pathway Construction and Implementation on Patient Outcomes in Anesthesia Recovery

The application of the clinical nursing pathway in the anesthesia recovery room is of great significance for improving nursing quality and reducing the ...

Gansu University Of Chinese Medicine

&#x9633;&#x6027;&#x6401;&#x6D45; RNA &#x75C5;&#x6BD2;&#x5916;&#x58F3;&#x86CB;&#x767D;&#x901A;&#x5E38;&#x5305;&#x542B;&#x4E00;&#x4E2A;&#x6B63;&#x7535;&#x8377;&#x7684;&#x5C3E;&#x5DF4;&#x5EF6;&#x4F38;&#x5411;&#x8863;&#x58F3;&#x7684;&#x4E2D;&#x5FC3;&#xFF0C;&#x4E0E;&#x75C5;&#x6BD2;&#x7684;&#x57FA;&#x56E0;&#x7EC4;&#x8FDB;&#x884C;&#x4EA4;&#x4E92;&#x3002;&#x5C3E;&#x90E8;&#x4E0E; RNA &#x4E4B;&#x95F4;&#x7684;&#x9759;&#x7535;&#x76F8;&#x4E92;&#x4F5C;&#x7528;&#x5047;&#x5B9A;&#x4F5C;&#x4E3A;&#x75C5;&#x6BD2;&#x5927;&#x4F1A;&#x548C;&#x7A33;&#x5B9A;&#x7684;&#x4E3B;&#x8981;&#x529B;&#x91CF;&#x3002;&#x8FD9;&#x9879;&#x5DE5;&#x4F5C;&#x7684;&#x76EE;&#x6807;&#x662F;&#x5BA1;&#x67E5;&#x9759;&#x7535;&#x76F8;&#x4E92;&#x4F5C;&#x7528;&#x548C;&#x6253;&#x5305;&#x4E09;&#x65B9;&#x65E0;&#x8292;&#x96C0;&#x82B1;&#x53F6;&#x75C5;&#x6BD2; (&#x4E18;) RNA &#x7684;&#x91CF;&#x4E4B;&#x95F4;&#x7684;&#x76F8;&#x4E92;&#x5173;&#x7CFB;&#x3002;&#x7EB3;&#x7C73;&#x538B;&#x75D5;&#x5B9E;&#x9A8C;&#x4F7F;&#x7528;&#x539F;&#x5B50;&#x529B;&#x663E;&#x5FAE;&#x955C;&#x663E;&#x793A;&#x4E18; virions &#x4E0E;&#x7ECF;&#x7531;&#x4E00;&#x7CFB;&#x5217;&#x7684;&#x4E0D;&#x540C; RNAs &#x7684;&#x521A;&#x5EA6;&#xFF0C;&#x662F;&#x6BD4;&#xFF0C;&#x5C06;&#x7531;&#x9759;&#x7535;&#x9884;&#x6D4B;&#x9AD8;&#x51FA; 10 &#x500D;&#x3002;&#x4E18;&#x7A81;&#x53D8;&#x4F53;&#x51CF;&#x5C11;&#x7A81;&#x53D8;&#x4F53;&#x589E;&#x52A0;&#x79EF;&#x6781;&#x6536;&#x8D39;&#x6253;&#x5305; &sim;900 &#x65B0;&#x53F0;&#x5E01;&#x7684;&#x989D;&#x5916; RNAs &#x7684; RNA encapsidated &#x79EF;&#x6781;&#x6536;&#x8D39;&#x8F83;&#x4F4E;&#x7684;&#x6570;&#x989D;&#x3002;&#x7136;&#x800C;&#xFF0C;&#x5305;&#x62EC;&#x989D;&#x5916; RNAs &#x622A;&#x65AD;&#x4E18; RNAs&#xFF0C;RNA4&#x3001; &#x6F5C;&#x5728;&#x7684;&#x7EC6;&#x80DE; Rna &#x6216;&#x8FD9;&#x4E09;&#x8005;&#x7ED3;&#x5408;&#x7684;&#x989D;&#x5916;&#x526F;&#x672C;&#x6307;&#x793A;&#x7684;&#x8863;&#x58F3;&#x7535;&#x8377;&#x7684;&#x53D8;&#x5316;&#x53EF;&#x80FD;&#x5BFC;&#x81F4;&#x5728;&#x51E0;&#x4E2A;&#x4E0D;&#x540C;&#x7684;&#x7ED3;&#x679C;&#xFF0C;&#x5728; RNA &#x8863;&#x58F3;&#x3002;&#x6B64;&#x5916;&#xFF0C;&#x7A81;&#x53D8;&#x4F53;&#x4E0E;&#x7279;&#x5B9A;&#x7684; arginines &#x6539;&#x4E3A; lysines &#x8863;&#x58F3;&#x4E2D;&#x8FD8;&#x5C55;&#x51FA;&#x4E86;&#x4E18; RNA4 &#x5177;&#x4F53;&#x8863;&#x58F3;&#x4E2D;&#x7684;&#x7F3A;&#x9677;&#x3002;&#x5B9E;&#x9A8C;&#x7ED3;&#x679C;&#x8868;&#x660E;&#xFF0C;&#x9759;&#x7535;&#x662F; RNA &#x8863;&#x58F3;&#x4E2D;&#x7684;&#x4E00;&#x4E2A;&#x4E3B;&#x8981;&#x7EC4;&#x4EF6;&#xFF0C;&#x4F46;&#x5374;&#x65E0;&#x6CD5;&#x5E10;&#x6237;&#x4E2D;&#x7684;&#x6240;&#x6709; RNA &#x8863;&#x58F3;&#x5BF9;&#x89C2;&#x5BDF;&#x5230;&#x7684;&#x5F71;&#x54CD;&#x3002;&#x7EB3;&#x5165;&#x9759;&#x7535;&#x7684;&#x70ED;&#x529B;&#x5B66;&#x5EFA;&#x6A21;&#x662F;&#x80FD;&#x591F;&#x9884;&#x6D4B;&#x7684; RNA &#x662F; encapsidated &#x4E3A;&#x5927;&#x591A;&#x6570;&#x7A81;&#x53D8;&#x4F53; virions&#xFF0C;&#x800C;&#x4E0D;&#x662F;&#x7A81;&#x53D8;&#x4F53;&#x4E0E;&#x6781;&#x7AEF;&#x805A;&#x96C6;&#x6B63;&#x7535;&#x8377;&#x8FD1;&#x4F3C;&#x957F;&#x5EA6;&#x3002;&#x4F4E;&#x6E29;&#x7535;&#x955C;&#x6280;&#x672F;&#x7684; virions RNA4 &#x989D;&#x5916;&#x526F;&#x672C;&#x900F;&#x9732;&#xFF0C;&#x5C3D;&#x7BA1;&#x5728; RNA encapsidated &#x589E;&#x52A0;&#xFF0C;&#x8863;&#x58F3;&#x7ED3;&#x6784;&#x5FAE;&#x6539;&#x53D8;&#x7684; encapsidated&#x3002;&#x8FD9;&#x4E9B;&#x7ED3;&#x679C;&#x901A;&#x8FC7;&#x5B9E;&#x9A8C;&#x8868;&#x660E;&#x9759;&#x7535;&#x548C;&#x4E18; RNAs &#x7684;&#x8863;&#x58F3;&#x989D;&#x5916;&#x9650;&#x5236;&#x7684;&#x5F71;&#x54CD;&#xFF0C;&#x53EF;&#x9002;&#x7528;&#x4E8E;&#x5176;&#x4ED6;&#x75C5;&#x6BD2;&#x3002;

&#x65E0;&#x8292;&#x96C0;&#x82B1;&#x53F6;&#x75C5;&#x6BD2;&#x5916;&#x58F3;&#x86CB;&#x767D;&#x7684; N-&#x672B;&#x7AEF;&#x548C; encapsidated RNAs &#x7684;&#x9759;&#x7535;&#x76F8;&#x4E92;&#x4F5C;&#x7528;&#x7684;&#x4E00;&#x79CD;&#x8003;&#x8BD5;&#x3002;

The coat protein of positive-stranded RNA viruses often contains a positively charged tail that extends toward the center of the capsid and interacts with the viral genome. Electrostatic interaction between the tail and the RNA has been postulated as a major force in virus assembly and stabilization. The goal of this work is to examine the correlation between electrostatic interaction and amount of RNA packaged in the tripartite Brome Mosaic Virus (BMV). Nanoindentation experiment using atomic force microscopy showed that the stiffness of BMV virions with different RNAs varied by a range that is 10-fold higher than that would be predicted by electrostatics. BMV mutants with decreased positive charges encapsidated lower amounts of RNA while mutants with increased positive charges packaged additional RNAs up to âˆ¼900 nt. However, the extra RNAs included truncated BMV RNAs, an additional copy of RNA4, potential cellular RNAs, or a combination of the three, indicating that change in the charge of the capsid could result in several different outcomes in RNA encapsidation. In addition, mutant with specific arginines changed to lysines in the capsid also exhibited defects in the specific encapsidation of BMV RNA4. The experimental results indicate that electrostatics is a major component in RNA encapsidation but was unable to account for all of the observed effects on RNA encapsidation. Thermodynamic modeling incorporating the electrostatics was able to predict the approximate length of the RNA to be encapsidated for the majority of mutant virions, but not for a mutant with extreme clustered positive charges. Cryo-electron microscopy of virions that encapsidated an additional copy of RNA4 revealed that, despite the increase in RNA encapsidated, the capsid structure was minimally changed. These results experimentally demonstrated the impact of electrostatics and additional restraints in the encapsidation of BMV RNAs, which could be applicable to other viruses.

An examination of the electrostatic interactions between the N-terminal tail of the Brome Mosaic Virus coat protein and encapsidated RNAs.

La prot&eacute;ine de capside du virus &agrave; ARN positif brin contient souvent une queue charg&eacute;e positivement qui s&#39;&eacute;tend vers le centre de la capside et interagit avec le g&eacute;nome viral. Interaction &eacute;lectrostatique entre la queue et l&#39;ARN a &eacute;t&eacute; postul&eacute;e comme une force majeure dans l&#39;Assembl&eacute;e de virus et de stabilisation. L&#39;objectif de ce travail est d&#39;&eacute;tudier la corr&eacute;lation entre l&#39;interaction &eacute;lectrostatique et de la quantit&eacute; d&#39;ARN emball&eacute; dans le Virus de mosa&iuml;que des Brome tripartite (BMV). Nanoindentation exp&eacute;rience utilisant atomique force microscope a montr&eacute; que la rigidit&eacute; des virions BMV avec diff&eacute;rents ARN modifi&eacute;e par une gamme qui est 10 fois plus haut que qui pourrait &ecirc;tre pr&eacute;vu par &eacute;lectrostatique. Mutants BMV avec diminu&eacute; des charges positives encapsid&eacute;s quantit&eacute;s plus faibles d&#39;ARN tandis que mutants ayant augment&eacute; positif frais emball&eacute;s RNAs suppl&eacute;mentaires jusqu&#39;&agrave; &sim;900 nt. Cependant, l&#39;ARN suppl&eacute;mentaires inclus tronqu&eacute;e BMV RNAs, un exemplaire suppl&eacute;mentaire du RNA4, ARN cellulaire potentielle ou une combinaison des trois, indiquant que changement dans la charge de la capside pourrait aboutir &agrave; des r&eacute;sultats diff&eacute;rents plusieurs dans RNA encapsidation. En outre, mutant avec des arginines chang&eacute; &agrave; lysines dans la capside expose aussi les d&eacute;fauts de l&#39;encapsidation sp&eacute;cifique de BMV RNA4. Les r&eacute;sultats exp&eacute;rimentaux indiquent qu&#39;&eacute;lectrostatique est une composante majeure de la RNA encapsidation mais n&#39;a pas pu tenir compte de tous les effets observ&eacute;s sur RNA encapsidation. Mod&eacute;lisation thermodynamique int&eacute;grant l&#39;&eacute;lectrostatique a &eacute;t&eacute; capable de pr&eacute;dire la dur&eacute;e approximative de l&#39;ARN d&#39;&ecirc;tre encapsid&eacute;s pour la majorit&eacute; des virions mutantes, mais pas pour un mutant avec des charges positives en cluster extr&ecirc;mes. Cryo microscopie &eacute;lectronique des virions qui encapsid&eacute;s une copie suppl&eacute;mentaire de RNA4 a r&eacute;v&eacute;l&eacute; que, malgr&eacute; l&#39;augmentation de RNA encapsid&eacute;s, la structure de la capside a &eacute;t&eacute; tr&egrave;s peu chang&eacute;e. Ces r&eacute;sultats ont d&eacute;montr&eacute; exp&eacute;rimentalement l&#39;impact de l&#39;&eacute;lectrostatique et des restrictions suppl&eacute;mentaires &agrave; l&#39;encapsidation de BMV RNAs, qui pourrait s&#39;appliquer &agrave; d&#39;autres virus.

Un examen des interactions &eacute;lectrostatiques entre la queue N-terminale de la prot&eacute;ine de capside du Virus de mosa&iuml;que de Brome et encapsid&eacute;s ARN.

La proteina del cappotto di virus a RNA filamento positivo contiene spesso una coda caricata positivamente che si estende verso il centro del capside e interagisce con il genoma virale. Interazione elettrostatica tra la coda e il RNA &egrave; stata postulata come una forza importante nell&#39;assemblaggio del virus e la stabilizzazione. L&#39;obiettivo di questo lavoro &egrave; quello di esaminare la correlazione tra interazioni elettrostatiche e la quantit&agrave; di RNA confezionato in tripartito Brome Mosaic Virus (BMV). Nanoindentazione esperimento atomico imporre microscopia ha mostrata che la rigidit&agrave; dei virions BMV con diversi RNAs varia a seconda di una serie che &egrave; 10 volte pi&ugrave; alto di quello che sarebbe previsto di elettrostatica. Mutanti BMV con diminuito cariche positive incapsidato bassa quantit&agrave; di RNA mentre mutanti con aumentato positivo oneri confezionato RNAs supplementari fino a &sim;900 nt. Tuttavia, il RNAs extra incluso troncato BMV RNAs, un&#39;ulteriore copia di RNA4, potenziali RNAs cellulare o una combinazione dei tre, che indica che cambia la carica il capside pu&ograve; comportare diversi risultati differenti in RNA encapsidation. Inoltre, mutante con specifiche ARGININE cambiato in Lisine nel capside esposto anche difetti il encapsidation specifico di BMV RNA4. I risultati sperimentali indicano che elettrostatica &egrave; un importante componente di RNA encapsidation ma era impossibile tenere conto di tutti gli effetti osservati su RNA encapsidation. Modellazione termodinamica che incorporano l&#39;elettrostatica &egrave; stato in grado di predire la lunghezza approssimativa del RNA di essere incapsidato per la maggior parte dei virioni mutante, ma non per un mutante con estreme cariche positive in cluster. Crio-microscopia elettronica di virioni che incapsidato una copia aggiuntiva di RNA4 ha rivelato che, nonostante l&#39;aumento di RNA incapsidato, la struttura del capside &egrave; stato minimamente modificata. Questi risultati sperimentalmente dimostrato l&#39;impatto dell&#39;elettrostatica e restrizioni aggiuntive in encapsidation di BMV RNAs, che potrebbero essere applicabili ad altri virus.

Un esame delle interazioni elettrostatiche tra la coda del N-terminale della proteina Brome Mosaic Virus coat e incapsidato RNAs.

&#x80AF;&#x5B9A;&#x7684;&#x306A;&#x9396; RNA &#x30A6;&#x30A4;&#x30EB;&#x30B9;&#x306E;&#x5916;&#x88AB;&#x86CB;&#x767D;&#x8CEA;&#x306B;&#x306F;&#x30AD;&#x30E3;&#x30D7;&#x30B7;&#x30C9;&#x306E;&#x4E2D;&#x5FC3;&#x306B;&#x5411;&#x304B;&#x3063;&#x3066;&#x3092;&#x62E1;&#x5F35;&#x3057;&#x3001;&#x30A6;&#x30A4;&#x30EB;&#x30B9;&#x306E;&#x30B2;&#x30CE;&#x30E0;&#x3068;&#x76F8;&#x4E92;&#x4F5C;&#x7528;&#x3059;&#x308B;&#x8377;&#x96FB;&#x5C3E;&#x304C;&#x542B;&#x307E;&#x308C;&#x307E;&#x3059;&#x3002;&#x9759;&#x96FB;&#x76F8;&#x4E92;&#x4F5C;&#x7528;&#x3001;&#x5C3E;&#x3068; RNA &#x30A6;&#x30A4;&#x30EB;&#x30B9; &#x30A2;&#x30BB;&#x30F3;&#x30D6;&#x30EA;&#x3068;&#x5B89;&#x5B9A;&#x5316;&#x306E;&#x4E3B;&#x8981;&#x306A;&#x529B;&#x3068;&#x3057;&#x3066;&#x4EEE;&#x5B9A;&#x3055;&#x308C;&#x3066;&#x3044;&#x307E;&#x3059;&#x3002;&#x3053;&#x306E;&#x4F5C;&#x696D;&#x306E;&#x76EE;&#x7684;&#x306F;&#x3001;&#x9759;&#x96FB;&#x7684;&#x76F8;&#x4E92;&#x4F5C;&#x7528;&#x3068;&#x4E09;&#x8005;&#x306E;&#x30D6;&#x30ED;&#x30E0; &#x30E2;&#x30B6;&#x30A4;&#x30AF; &#x30A6;&#x30A4;&#x30EB;&#x30B9; (BMV) &#x306E;&#x30D1;&#x30C3;&#x30B1;&#x30FC;&#x30B8;&#x5316;&#x3055;&#x308C;&#x305F; RNA &#x306E;&#x91CF;&#x306E;&#x76F8;&#x95A2;&#x3092;&#x8ABF;&#x3079;&#x308B;&#x3053;&#x3068;&#x3067;&#x3059;&#x3002;&#x30CA;&#x30CE;&#x30A4;&#x30F3;&#x30C7;&#x30F3;&#x30C6;&#x30FC;&#x30B7;&#x30E7;&#x30F3;&#x5B9F;&#x9A13;&#x539F;&#x5B50;&#x3092;&#x7528;&#x3044;&#x305F;&#x9855;&#x5FAE;&#x93E1;&#x306F;&#x7BC4;&#x56F2;&#x306B;&#x3088;&#x3063;&#x3066;&#x69D8;&#x3005; &#x306A;&#x7570;&#x306A;&#x308B; Rna &#x304C; BMV &#x7C92;&#x5B50;&#x306E;&#x525B;&#x6027;&#x306F;&#x3001;&#x9759;&#x96FB;&#x6C17;&#x306B;&#x3088;&#x3063;&#x3066;&#x4E88;&#x6E2C;&#x3055;&#x308C;&#x308B;&#x3088;&#x308A;&#x3082;&#x9AD8;&#x3044; 10 &#x500D;&#x3042;&#x308B;&#x3053;&#x3068;&#x3092;&#x793A;&#x3057;&#x305F;&#x3002;&#x5909;&#x7570;&#x4F53;&#x3068;&#x80AF;&#x5B9A;&#x7684;&#x306A;&#x6599;&#x91D1;&#x305D;&#x306E;&#x4ED6;&#x306E; Rna &sim;900 nt &#x307E;&#x3067;&#x30D1;&#x30C3;&#x30B1;&#x30FC;&#x30B8;&#x304C; BMV &#x5909;&#x7570;&#x4F53;&#x3068; RNA &#x306E;&#x6B63;&#x96FB;&#x8377;&#x4EE5;&#x4E0A;&#x4F4E;&#x3044;&#x91CF;&#x3092;&#x6E1B;&#x5C11;&#x3057;&#x307E;&#x3057;&#x305F;&#x3002;&#x305F;&#x3060;&#x3057;&#x3001;&#x542B;&#x307E;&#x308C;&#x3066;&#x3044;&#x308B;&#x4F59;&#x5206;&#x306A; Rna BMV Rna&#x3001;RNA4&#x3001;&#x6F5C;&#x5728;&#x7684;&#x306A;&#x7D30;&#x80DE;&#x5185; Rna &#x306E;&#x307E;&#x305F;&#x306F;&#x3001;3 &#x3064;&#x306E;&#x7D44;&#x307F;&#x5408;&#x308F;&#x305B;&#x306E;&#x8FFD;&#x52A0;&#x306E;&#x30B3;&#x30D4;&#x30FC;&#x306F;&#x30AD;&#x30E3;&#x30D7;&#x30B7;&#x30C9;&#x306E;&#x62C5;&#x5F53;&#x5909;&#x66F4;&#x3044;&#x304F;&#x3064;&#x304B;&#x306E;&#x7570;&#x306A;&#x308B;&#x7D50;&#x679C; RNA &#x306E;&#x8A66;&#x9A13;&#x7BA1;&#x5185;&#x3067;&#x53EF;&#x80FD;&#x6027;&#x304C;&#x3042;&#x308B;&#x3053;&#x3068;&#x3092;&#x793A;&#x3059;&#x5207;&#x308A;&#x6368;&#x3066;&#x304F;&#x3060;&#x3055;&#x3044;&#x3002;&#x307E;&#x305F;&#x3001;&#x5909;&#x7570;&#x682A;&#x306E;&#x30AB;&#x30D7;&#x30B7;&#x30C9; 24arc-4 &#x306B;&#x5909;&#x66F4;&#x7279;&#x5B9A;&#x306E;&#x30A2;&#x30EB;&#x30AE;&#x30CB;&#x30F3;&#x306F;&#x307E;&#x305F;&#x7279;&#x5B9A;&#x306E;&#x8A66;&#x9A13;&#x7BA1; BMV RNA4 &#x306E;&#x6B20;&#x9665;&#x3092;&#x5C55;&#x793A;&#x3057;&#x307E;&#x3057;&#x305F;&#x3002;&#x5B9F;&#x9A13;&#x306E;&#x7D50;&#x679C;&#x306B;&#x306F;&#x3001;&#x305D;&#x306E;&#x9759;&#x96FB; RNA &#x306E;&#x8A66;&#x9A13;&#x7BA1;&#x3067;&#x306E;&#x4E3B;&#x8981;&#x306A;&#x30B3;&#x30F3;&#x30DD;&#x30FC;&#x30CD;&#x30F3;&#x30C8;&#x304C;&#x3059;&#x3079;&#x3066;&#x306E; RNA &#x306E;&#x8A66;&#x9A13;&#x7BA1;&#x306B;&#x89B3;&#x6E2C;&#x3055;&#x308C;&#x305F;&#x52B9;&#x679C;&#x3092;&#x8003;&#x616E;&#x3059;&#x308B;&#x3053;&#x3068;&#x304C;&#x3067;&#x304D;&#x307E;&#x305B;&#x3093;&#x3067;&#x3057;&#x305F;&#x3092;&#x793A;&#x3057;&#x307E;&#x3059;&#x3002;&#x71B1;&#x529B;&#x5B66;&#x7684;&#x30E2;&#x30C7;&#x30EA;&#x30F3;&#x30B0;&#x3001;&#x9759;&#x96FB;&#x6C17;&#x3092;&#x7D44;&#x307F;&#x8FBC;&#x3080;&#x4EE5;&#x4E0A;&#x5909;&#x7570;&#x30A6;&#x30A4;&#x30EB;&#x30B9;&#x7C92;&#x5B50;&#x306E;&#x5927;&#x534A;&#x306E;&#x305F;&#x3081;&#x3067;&#x306F;&#x306A;&#x304F;&#x3001;&#x6975;&#x7AEF;&#x306A;&#x30AF;&#x30E9;&#x30B9;&#x30BF;&#x30FC;&#x5316;&#x3055;&#x308C;&#x305F;&#x6B63;&#x96FB;&#x8377;&#x3092;&#x5909;&#x7570;&#x4F53;&#x306B; RNA &#x306E;&#x304A;&#x304A;&#x3088;&#x305D;&#x306E;&#x9577;&#x3055;&#x3092;&#x4E88;&#x6E2C;&#x3059;&#x308B;&#x3053;&#x3068;&#x304C;&#x3067;&#x304D;&#x305F;&#x3002;&#x30A6;&#x30A4;&#x30EB;&#x30B9;&#x7C92;&#x5B50;&#x306E;&#x4F4E;&#x6E29;&#x96FB;&#x5B50;&#x9855;&#x5FAE;&#x93E1;&#x89B3;&#x5BDF; RNA4 &#x306E;&#x8FFD;&#x52A0;&#x30B3;&#x30D4;&#x30FC;&#x3092;&#x660E;&#x3089;&#x304B;&#x306B;&#x3057;&#x305F; RNA &#x4EE5;&#x4E0A;&#x306E;&#x5897;&#x52A0;&#x306B;&#x3082;&#x304B;&#x304B;&#x308F;&#x3089;&#x305A;&#x3001;&#x30AB;&#x30D7;&#x30B7;&#x30C9;&#x69CB;&#x9020;&#x3092;&#x6700;&#x5C0F;&#x9650;&#x306B;&#x5909;&#x66F4;&#x3055;&#x308C;&#x305F;&#x3053;&#x3068;&#x3001;&#x305D;&#x308C;&#x4EE5;&#x4E0A;&#x3002;&#x3053;&#x308C;&#x3089;&#x306E;&#x7D50;&#x679C;&#x304B;&#x3089;&#x9759;&#x96FB;&#x6C17;&#x3084; BMV Rna &#x306E;&#x8A66;&#x9A13;&#x7BA1;&#x306B;&#x8FFD;&#x52A0;&#x306E;&#x5236;&#x7D04;&#x306E;&#x5F71;&#x97FF;&#x306F;&#x3001;&#x4ED6;&#x306E;&#x30A6;&#x30A4;&#x30EB;&#x30B9;&#x306B;&#x9069;&#x7528;&#x3067;&#x304D;&#x308B;&#x5B9F;&#x9A13;&#x3002;

&#x30D6;&#x30ED;&#x30E0; &#x30E2;&#x30B6;&#x30A4;&#x30AF; &#x30A6;&#x30A4;&#x30EB;&#x30B9;&#x5916;&#x88AB;&#x86CB;&#x767D;&#x8CEA;&#x306E; N &#x672B;&#x7AEF;&#x3068;&#x4EE5;&#x4E0A; Rna &#x9593;&#x306E;&#x9759;&#x96FB;&#x76F8;&#x4E92;&#x4F5C;&#x7528;&#x306E;&#x691C;&#x8A0E;&#x3002;

&#xC591;&#xC131; &#xAC00;&#xB2E5; RNA &#xBC14;&#xC774;&#xB7EC;&#xC2A4;&#xC758; &#xC678; &#xD22C; &#xB2E8;&#xBC31;&#xC9C8;&#xC5D0;&#xC11C; capsid &#xC911;&#xC559; &#xCABD;&#xC73C;&#xB85C; &#xD655;&#xC7A5; &#xD558; &#xACE0; &#xBC14;&#xC774;&#xB7EC;&#xC2A4; &#xC131; &#xAC8C;&#xB188;&#xC640; &#xC0C1;&#xD638; &#xC791;&#xC6A9; &#xD558;&#xB294; &#xAE0D;&#xC815;&#xC801;&#xC73C;&#xB85C; &#xCDA9;&#xC804;&#xB41C; &#xAF2C;&#xB9AC;&#xB97C; &#xB4E4;&#xC5B4; &#xC788;&#xC2B5;&#xB2C8;&#xB2E4;. &#xAF2C;&#xB9AC;&#xC640; RNA &#xC0AC;&#xC774;&#xC758; &#xC815;&#xC804;&#xAE30; &#xC0C1;&#xD638; &#xC791;&#xC6A9;&#xC740; &#xBC14;&#xC774;&#xB7EC;&#xC2A4; &#xC5B4;&#xC148;&#xBE14;&#xB9AC; &#xBC0F; &#xC548;&#xC815;&#xD654;&#xC5D0; &#xD070; &#xD798;&#xC73C;&#xB85C; postulated &#xB418;&#xC5C8;&#xC2B5;&#xB2C8;&#xB2E4;. &#xC774; &#xC791;&#xD488;&#xC758; &#xBAA9;&#xC801;&#xC740; &#xC815;&#xC804;&#xAE30; &#xC0C1;&#xD638; &#xC791;&#xC6A9; &#xBC0F; &#xB178;&#xC0AC; &#xC815; &#xBE0C;&#xB86C; &#xBAA8;&#xC790;&#xC774;&#xD06C; &#xBC14;&#xC774;&#xB7EC;&#xC2A4; (BMV)&#xC5D0; &#xD3EC;&#xC7A5; &#xD558;&#xB294; RNA&#xC758; &#xC591;&#xC744; &#xAC04;&#xC758; &#xC0C1;&#xAD00; &#xAD00;&#xACC4;&#xB97C; &#xAC80;&#xD1A0; &#xD558;&#xB294; &#xAC83;. &#xB098;&#xB178; &#xC2E4;&#xD5D8; &#xC0AC;&#xC6A9; &#xC6D0;&#xC790; &#xD604;&#xBBF8;&#xACBD;&#xC744; &#xBCF4;&#xC5EC;&#xC8FC;&#xC5C8;&#xB2E4;&#xB294; &#xBC94;&#xC704;&#xC5D0; &#xC758;&#xD574; &#xB2E4;&#xC591; &#xD55C; &#xB2E4;&#xB978; Rnas&#xC640; BMV virions &#xAC15;&#xC131; &#xC788;&#xB294; &#xC0AC;&#xC9C4; &#xADF8; &#xB354;&#xD574;&#xC9C0;&#xBA74;&#xC5D0; &#xC758;&#xD574; &#xC608;&#xCE21; &#xB420; &#xAC83; &#xBCF4;&#xB2E4; &#xB354; &#xB192;&#xC740; &#xAC15;&#xC81C;. BMV &#xB3CC;&#xC5F0;&#xBCC0;&#xC774;&#xC640; &#xB3CC;&#xC5F0;&#xBCC0;&#xC774;&#xC640; &#xAE0D;&#xC815;&#xC801;&#xC778; &#xC694;&#xAE08; &#xD328;&#xD0A4;&#xC9C0; &sim;900 nt &#xCD5C;&#xB300; &#xCD94;&#xAC00; RNAs &#xC99D;&#xAC00; &#xD558;&#xBA74;&#xC11C; &#xAE0D;&#xC815;&#xC801;&#xC778; &#xC694;&#xAE08; encapsidated &#xB0AE;&#xC740; &#xC591;&#xC758; RNA &#xAC10;&#xC18C;. &#xADF8;&#xB7EC;&#xB098;, &#xD3EC;&#xD568; &#xC5EC;&#xBD84;&#xC758; RNAs &#xC798;&#xB9B0; BMV RNAs, RNA4, &#xC7A0;&#xC7AC;&#xC801;&#xC778; &#xC140;&#xB8F0;&#xB7EC; RNAs &#xB610;&#xB294; &#xC138;&#xC758; &#xC870;&#xD569; &#xCD94;&#xAC00; &#xBCF5;&#xC0AC;&#xBCF8;&#xC744; &#xB098;&#xD0C0;&#xB0B4;&#xB294; RNA encapsidation&#xC5D0;&#xC11C; &#xC5EC;&#xB7EC; &#xAC00;&#xC9C0; &#xB2E4;&#xB978; &#xACB0;&#xACFC; capsid &#xB2F4;&#xB2F9;&#xC5D0;&#xC11C; &#xBCC0;&#xACBD; &#xB420; &#xC218; &#xC788;&#xC2B5;&#xB2C8;&#xB2E4;. &#xB610;&#xD55C;, &#xD2B9;&#xC815; arginines&#xB294; capsid&#xC5D0;&#xC11C; lysines&#xB85C; &#xBCC0;&#xACBD; &#xB41C; &#xB3CC;&#xC5F0;&#xBCC0;&#xC774; &#xB610;&#xD55C; &#xACB0;&#xD568; BMV rna4&#xC758; &#xD2B9;&#xC815; encapsidation&#xC5D0;&#xB97C; &#xC804;&#xC2DC; &#xD558; &#xACE0;. &#xC2E4;&#xD5D8; &#xACB0;&#xACFC; &#xADF8; &#xB354;&#xD574;&#xC9C0;&#xBA74; RNA encapsidation&#xC758; &#xC8FC;&#xC694; &#xAD6C;&#xC131; &#xC694;&#xC18C; &#xC774;&#xC9C0;&#xB9CC; &#xBAA8;&#xB4E0; RNA encapsidation&#xC5D0; &#xAD00;&#xCC30; &#xB41C; &#xD6A8;&#xACFC; &#xCC28;&#xC9C0; &#xD558;&#xC9C0; &#xBABB;&#xD588;&#xC2B5;&#xB2C8;&#xB2E4; &#xB098;&#xD0C0;&#xB0C5;&#xB2C8;&#xB2E4;. &#xC5F4;&#xC5ED;&#xD559;&#xC801; &#xBAA8;&#xB378;&#xB9C1; &#xD569;&#xB2C8;&#xB2E4; &#xB354;&#xD574;&#xC9C0;&#xBA74; &#xD1B5;&#xD569; encapsidated &#xB3CC;&#xC5F0;&#xBCC0;&#xC774; virions&#xC758; &#xB300;&#xBD80;&#xBD84;&#xC5D0; &#xB300; &#xD55C; &#xD558;&#xC9C0;&#xB9CC; &#xADF9;&#xB2E8;&#xC801;&#xC778; &#xD074;&#xB7EC;&#xC2A4;&#xD130; &#xB41C; &#xAE0D;&#xC815;&#xC801;&#xC778; &#xC694;&#xAE08; &#xB3CC;&#xC5F0;&#xBCC0;&#xC774; &#xB420; RNA&#xC758; &#xB300;&#xB7B5;&#xC801;&#xC778; &#xAE38;&#xC774; &#xC608;&#xCE21;&#xD560; &#xC218; &#xC788;&#xC5C8;&#xB2E4;. &#xACF3;&#xC744; &#xC54C;&#xC544;&#xB0B4;&#xB294;-&#xC804;&#xC790; &#xD604;&#xBBF8;&#xACBD;&#xC758; virions&#xB294; encapsidated rna4&#xC758; &#xCD94;&#xAC00; &#xBCF5;&#xC0AC;&#xBCF8;&#xC744; &#xACF5;&#xAC1C;, RNA encapsidated &#xC99D;&#xAC00;&#xB3C4; &#xBD88;&#xAD6C; &#xD558; &#xACE0; capsid &#xAD6C;&#xC870; &#xBCC0;&#xACBD; &#xCD5C;&#xC18C;&#xD55C; &#xD588;&#xB2E4;. &#xC774;&#xB7EC;&#xD55C; &#xACB0;&#xACFC; &#xC2E4;&#xD5D8;&#xC801;&#xC73C;&#xB85C; &#xB354;&#xD574;&#xC9C0;&#xBA74; BMV Rnas&#xC758; encapsidation&#xC5D0; &#xCD94;&#xAC00; &#xC9C0;&#xC9C0;&#xB300;&#xC758; &#xC601;&#xD5A5;&#xC744; &#xC2DC;&#xC5F0; &#xD558; &#xC5EC; &#xB2E4;&#xB978; &#xBC14;&#xC774;&#xB7EC;&#xC2A4;&#xC5D0; &#xC801;&#xC6A9; &#xB420; &#xC218; &#xC788;&#xB294;.

&#xBE0C;&#xB86C; &#xBAA8;&#xC790;&#xC774;&#xD06C; &#xBC14;&#xC774;&#xB7EC;&#xC2A4; &#xC678; &#xD22C; &#xB2E8;&#xBC31;&#xC9C8;&#xC758; N-&#xD130;&#xBBF8;&#xB110; &#xAF2C;&#xB9AC;&#xC640; encapsidated RNAs &#xAC04;&#xC758; &#xC815;&#xC804;&#xAE30; &#xC0C1;&#xD638; &#xC791;&#xC6A9;&#xC758; &#xC2DC;&#xD5D8;.

La prote&iacute;na de la c&aacute;psida de los virus de ARN de cadena positiva a menudo contiene una carga positiva cola que se extiende hacia el centro de la c&aacute;pside e interacciona con el genoma viral. Interacci&oacute;n electrost&aacute;tica entre la cola y el RNA se ha postulado como una importante fuerza en la Asamblea de virus y estabilizaci&oacute;n. El objetivo de este trabajo es examinar la correlaci&oacute;n entre la interacci&oacute;n electrost&aacute;tica y cantidad de ARN envasado en el tripartito Brome Mosaic Virus (BMV). Nanoindentaci&oacute;n experimento utilizando at&oacute;mica fuerza microscopia demostr&oacute; que la rigidez de viriones BMV con diferentes RNAs variados por una gama que es 10 veces superior a la que se puede predecir por electrost&aacute;tica. Mutantes de la BMV con disminuci&oacute;n cantidades menores de cargas positivas genoma de ARN, mientras que mutantes con aumentaron positivo cargos empaquetan adicionales RNAs hasta &sim;900 nt. Sin embargo, el RNAs extra incluido truncado BMV RNAs, una copia adicional de RNA4, RNAs celulares potenciales o una combinaci&oacute;n de los tres, lo que indica que el cambio de los cargos de la c&aacute;pside podr&iacute;a resultar en varios resultados diferentes en RNA encapsidation. Adem&aacute;s, mutante con arginines espec&iacute;ficos cambiada a lisinas en la c&aacute;pside tambi&eacute;n exhibi&oacute; defectos en la encapsidation espec&iacute;fica de RNA4 de la BMV. Los resultados experimentales indican eso electrost&aacute;tica es un componente importante en RNA encapsidation pero fue incapaz de explicar todos los efectos observados en RNA encapsidation. Modelado termodin&aacute;mico incorporando la electrost&aacute;tica fue capaz de predecir la longitud aproximada del ARN que encapsula la mayor&iacute;a de viriones mutantes, pero no para un mutante con cargas positivas agrupados extremas. Crio-microscop&iacute;a de viriones que encapsula una copia adicional de RNA4 revel&oacute; que, a pesar del aumento en el genoma de RNA, la estructura de la c&aacute;pside m&iacute;nimamente cambi&oacute;. Estos resultados demostraron experimentalmente los efectos de la electrost&aacute;tica y las restricciones adicionales en el encapsidation de RNAs de la BMV, que podr&iacute;a ser aplicable a otros virus.

Un examen de las interacciones electrost&aacute;ticas entre la cola del N-terminal de la prote&iacute;na de la c&aacute;psida de Brome Mosaic Virus y genoma ARN.

The capacity of numerous bacterial species to tolerate antibiotics and other toxic compounds arises in part from the activity of energy-dependent transporters. In Gram-negative bacteria, many of these transporters form multicomponent 'pumps' that span both inner and outer membranes and are driven energetically by a primary or secondary transporter component. A model system for such a pump is the acridine resistance complex of Escherichia coli. This pump assembly comprises the outer-membrane channel TolC, the secondary transporter AcrB located in the inner membrane, and the periplasmic AcrA, which bridges these two integral membrane proteins. The AcrAB-TolC efflux pump is able to transport vectorially a diverse array of compounds with little chemical similarity, thus conferring resistance to a broad spectrum of antibiotics. Homologous complexes are found in many Gram-negative species, including in animal and plant pathogens. Crystal structures are available for the individual components of the pump and have provided insights into substrate recognition, energy coupling and the transduction of conformational changes associated with the transport process. However, how the subunits are organized in the pump, their stoichiometry and the details of their interactions are not known. Here we present the pseudo-atomic structure of a complete multidrug efflux pump in complex with a modulatory protein partner from E. coli. The model defines the quaternary organization of the pump, identifies key domain interactions, and suggests a cooperative process for channel assembly and opening. These findings illuminate the basis for drug resistance in numerous pathogenic bacterial species. 

Structure of the AcrAB-TolC multidrug efflux pump.

Advances in electron cryo-microscopy have enabled structure determination of macromolecules at near-atomic resolution. However, structure determination, even using de novo methods, remains susceptible to model bias and overfitting. Here we describe a complete workflow for data acquisition, image processing, all-atom modelling and validation of brome mosaic virus, an RNA virus. Data were collected with a direct electron detector in integrating mode and an exposure beyond the traditional radiation damage limit. The final density map has a resolution of 3.8 Å as assessed by two independent data sets and maps. We used the map to derive an all-atom model with a newly implemented real-space optimization protocol. The validity of the model was verified by its match with the density map and a previous model from X-ray crystallography, as well as the internal consistency of models from independent maps. This study demonstrates a practical approach to obtain a rigorously validated atomic resolution electron cryo-microscopy structure. 

An atomic model of brome mosaic virus using direct electron detection and real-space optimization.

Estrogen receptor (ER/ESR1) is a transcription factor critical for development, reproduction, metabolism, and cancer. ER function hinges on its ability to recruit primary and secondary coactivators, yet structural information on the full-length receptor-coactivator complex to complement preexisting and sometimes controversial biochemical information is lacking. Here, we use cryoelectron microscopy (cryo-EM) to determine the quaternary structure of an active complex of DNA-bound ERα, steroid receptor coactivator 3 (SRC-3/NCOA3), and a secondary coactivator (p300/EP300). Our structural model suggests the following assembly mechanism for the complex: each of the two ligand-bound ERα monomers independently recruits one SRC-3 protein via the transactivation domain of ERα; the two SRC-3s in turn bind to different regions of one p300 protein through multiple contacts. We also present structural evidence for the location of activation function 1 (AF-1) in a full-length nuclear receptor, which supports a role for AF-1 in SRC-3 recruitment. 

Structure of a biologically active estrogen receptor-coactivator complex on DNA.

The matrix protein 1 (M1) is the most abundant structural protein in influenza A virus particles. It oligomerizes to form the matrix layer under the lipid membrane, sustaining stabilization of the morphology of the virion. The present study indicates that M1 forms oligomers based on a fourfold symmetrical oligomerization pattern. Further analysis revealed that the oligomerization pattern of M1 was controlled by a highly conserved region within the C-terminal domain. Two polar residues of this region, serine-183 (S183) and threonine-185 (T185), were identified to be critical for the oligomerization pattern of M1. M1 point mutants suggest that single S183A or T185A substitution could result in the production of morphologically filamentous particles, while double substitutions, M1-S183A/T185A, totally disrupted the fourfold symmetry and resulted in the failure of virus production. These data indicate that the polar groups in these residues are essential to control the oligomerization pattern of M1. Thus, the present study will aid in determining the mechanisms of influenza A virus matrix layer formation during virus morphogenesis.

Two polar residues within C-terminal domain of M1 are critical for the formation of influenza A Virions.

Inositol-1,4,5-trisphosphate receptors (InsP3Rs) are ubiquitous ion channels responsible for cytosolic Ca(2+) signalling and essential for a broad array of cellular processes ranging from contraction to secretion, and from proliferation to cell death. Despite decades of research on InsP3Rs, a mechanistic understanding of their structure-function relationship is lacking. Here we present the first, to our knowledge, near-atomic (4.7 Å) resolution electron cryomicroscopy structure of the tetrameric mammalian type 1 InsP3R channel in its apo-state. At this resolution, we are able to trace unambiguously ∼85% of the protein backbone, allowing us to identify the structural elements involved in gating and modulation of this 1.3-megadalton channel. Although the central Ca(2+)-conduction pathway is similar to other ion channels, including the closely related ryanodine receptor, the cytosolic carboxy termini are uniquely arranged in a left-handed α-helical bundle, directly interacting with the amino-terminal domains of adjacent subunits. This configuration suggests a molecular mechanism for allosteric regulation of channel gating by intracellular signals. 

Gating machinery of InsP3R channels revealed by electron cryomicroscopy.

Electron cryomicroscopy (cryo-EM) has been used to determine the atomic coordinates (models) from density maps of biological assemblies. These models can be assessed by their overall fit to the experimental data and stereochemical information. However, these models do not annotate the actual density values of the atoms nor their positional uncertainty. Here, we introduce a computational procedure to derive an atomic model from a cryo-EM map with annotated metadata. The accuracy of such a model is validated by a faithful replication of the experimental cryo-EM map computed using the coordinates and associated metadata. The functional interpretation of any structural features in the model and its utilization for future studies can be made in the context of its measure of uncertainty. We applied this protocol to the 3.3-Å map of the mature P22 bacteriophage capsid, a large and complex macromolecular assembly. With this protocol, we identify and annotate previously undescribed molecular interactions between capsid subunits that are crucial to maintain stability in the absence of cementing proteins or cross-linking, as occur in other bacteriophages.

Accurate model annotation of a near-atomic resolution cryo-EM map.

Bacterial efflux pumps confer multidrug resistance by transporting diverse antibiotics from the cell. In Gram-negative bacteria, some of these pumps form multi-protein assemblies that span the cell envelope. Here, we report the near-atomic resolution cryoEM structures of the  AcrAB-TolC multidrug efflux pump in resting and drug transport states, revealing a quaternary structural switch that allosterically couples and synchronizes initial ligand binding with channel opening. Within the transport-activated state, the channel remains open even though the pump cycles through three distinct conformations. Collectively, our data provide a dynamic mechanism for the assembly and operation of the AcrAB-TolC pump.

An allosteric transport mechanism for the AcrAB-TolC multidrug efflux pump.

Nuclear receptors recruit multiple coactivators sequentially to activate transcription. This "ordered" recruitment allows different coactivator activities to engage the nuclear receptor complex at different steps of transcription. Estrogen receptor (ER) recruits steroid receptor coactivator-3 (SRC-3) primary coactivator and secondary coactivators, p300/CBP and CARM1. CARM1 recruitment lags behind the binding of SRC-3 and p300 to ER. Combining cryo-electron microscopy (cryo-EM) structure analysis and biochemical approaches, we demonstrate that there is a close crosstalk between early- and late-recruited coactivators. The sequential recruitment of CARM1 not only adds a protein arginine methyltransferase activity to the ER-coactivator complex, it also alters the structural organization of the pre-existing ERE/ERα/SRC-3/p300 complex. It induces a p300 conformational change and significantly increases p300 HAT activity on histone H3K18 residues, which, in turn, promotes CARM1 methylation activity on H3R17 residues to enhance transcriptional activity. This study reveals a structural role for a coactivator sequential recruitment and biochemical process in ER-mediated transcription.

Structural and Functional Impacts of ER Coactivator Sequential Recruitment.

The cell envelope of Gram-negative bacteria comprises an outer membrane, a cytoplasmic inner membrane, and an interstitial space. The tripartite multidrug transporter AcrAB-TolC, which uses proton electrochemical gradients to vectorially drive the efflux of drugs from the cell, spans this envelope. We describe here details of the methods used to prepare the recombinant tripartite assembly for high-resolution structure determination by cryo-EM.

Purification of AcrAB-TolC Multidrug Efflux Pump for Cryo-EM Analysis.

Inositol-1,4,5-trisphosphate receptors (InsPRs) are cation channels that mobilize Ca from intracellular stores in response to a wide range of cellular stimuli. The paradigm of InsPR activation is the coupled interplay between binding of InsP and Ca that switches the ion conduction pathway between closed and open states to enable the passage of Ca through the channel. However, the molecular mechanism of how the receptor senses and decodes ligand-binding signals into gating motion remains unknown. Here, we present the electron cryo-microscopy structure of InsPR1 from rat cerebellum determined to 4.1 Å resolution in the presence of activating concentrations of Ca and adenophostin A (AdA), a structural mimetic of InsP and the most potent known agonist of the channel. Comparison with the 3.9 Å-resolution structure of InsPR1 in the Apo-state, also reported herein, reveals the binding arrangement of AdA in the tetrameric channel assembly and striking ligand-induced conformational rearrangements within cytoplasmic domains coupled to the dilation of a hydrophobic constriction at the gate. Together, our results provide critical insights into the mechanistic principles by which ligand-binding allosterically gates InsPR channel.

Cryo-EM reveals ligand induced allostery underlying InsPR channel gating.

Cation channels of the transient receptor potential (TRP) family serve important physiological roles by opening in response to diverse intra- and extracellular stimuli that regulate their lower or upper gates. Despite extensive studies, the mechanism coupling these gates has remained obscure. Previous structures have failed to resolve extracellular loops, known in the TRPV subfamily as 'pore turrets', which are proximal to the upper gates. We established the importance of the pore turret through activity assays and by solving structures of rat TRPV2, both with and without an intact turret at resolutions of 4.0 Å and 3.6 Å, respectively. These structures resolve the full-length pore turret and reveal fully open and partially open states of TRPV2, both with unoccupied vanilloid pockets. Our results suggest a mechanism by which physiological signals, such as lipid binding, can regulate the lower gate and couple to the upper gate through a pore-turret-facilitated mechanism.

Structures of TRPV2 in distinct conformations provide insight into role of the pore turret.

Globally, human respiratory syncytial virus (RSV) is a leading cause of lower respiratory tract infections in newborns, young children, and the elderly for which there is no vaccine. The RSV fusion (F) glycoprotein is a major target for vaccine development. Here, we describe a novel monoclonal antibody (designated as R4.C6) that recognizes both pre-fusion and post-fusion RSV F, and binds with nanomole affinity to a unique neutralizing site comprised of antigenic sites II and IV on the globular head. A 3.9 Å-resolution structure of RSV F-R4.C6 Fab complex was obtained by single particle cryo-electron microscopy and 3D reconstruction. The structure unraveled detailed interactions of R4.C6 with antigenic site II on one protomer and site IV on a neighboring protomer of post-fusion RSV F protein. These findings significantly further our understanding of the antigenic complexity of the F protein and provide new insights into RSV vaccine design.

Structure basis of neutralization by a novel site II/IV antibody against respiratory syncytial virus fusion protein.

Multidrug efflux pumps actively expel a wide range of toxic substrates from the cell and play a major role in intrinsic and acquired drug resistance. In Gram-negative bacteria, these pumps form tripartite assemblies that span the cell envelope. However, the in situ structure and assembly mechanism of multidrug efflux pumps remain unknown. Here we report the in situ structure of the Escherichia coli AcrAB-TolC multidrug efflux pump obtained by electron cryo-tomography and subtomogram averaging. The fully assembled efflux pump is observed in a closed state under conditions of antibiotic challenge and in an open state in the presence of AcrB inhibitor. We also observe intermediate AcrAB complexes without TolC and discover that AcrA contacts the peptidoglycan layer of the periplasm. Our data point to a sequential assembly process in living bacteria, beginning with formation of the AcrAB subcomplex and suggest domains to target with efflux pump inhibitors.

In situ structure and assembly of the multidrug efflux pump AcrAB-TolC.

Single-stranded RNA bacteriophages (ssRNA phages) infect Gram-negative bacteria via a single maturation protein (Mat), which attaches to a retractile pilus of the host. Here we present structures of the ssRNA phage MS2 in complex with the Escherichia coli F-pilus, showing a network of hydrophobic and electrostatic interactions at the Mat-pilus interface. Moreover, binding of the pilus induces slight orientational variations of the Mat relative to the rest of the phage capsid, priming the Mat-connected genomic RNA (gRNA) for its release from the virions. The exposed tip of the attached Mat points opposite to the direction of the pilus retraction, which may facilitate the translocation of the gRNA from the capsid into the host cytosol. In addition, our structures determine the orientation of the assembled F-pilin subunits relative to the cell envelope, providing insights into the F-like type IV secretion systems.

Structural basis for the adsorption of a single-stranded RNA bacteriophage.

Electron cryotomography is currently the only method capable of visualizing cells in three dimensions at nanometer resolutions. While modern instruments produce massive amounts of tomography data containing extremely rich structural information, data processing is very labor intensive and the results are often limited by the skills of the personnel rather than the data. We present an integrated workflow that covers the entire tomography data processing pipeline, from automated tilt series alignment to subnanometer resolution subtomogram averaging. Resolution enhancement is made possible through the use of per-particle per-tilt contrast transfer function correction and alignment. The workflow greatly reduces human bias, increases throughput and more closely approaches data-limited resolution for subtomogram averaging in both purified macromolecules and cells.

A complete data processing workflow for cryo-ET and subtomogram averaging.

TrkH is a bacterial ion channel implicated in K uptake and pH regulation. TrkH assembles with its regulatory protein, TrkA, which closes the channel when bound to ADP and opens it when bound to ATP. However, it is unknown how nucleotides control the gating of TrkH through TrkA. Here we report the structures of the TrkH-TrkA complex in the presence of ADP or ATP. TrkA forms a tetrameric ring when bound to ADP and constrains TrkH to a closed conformation. The TrkA ring splits into two TrkA dimers in the presence of ATP and releases the constraints on TrkH, resulting in an open channel conformation. Functional studies show that both the tetramer-to-dimer conversion of TrkA and the loss of constraints on TrkH are required for channel gating. In addition, deletion of TrkA in Escherichia coli depolarizes the cell, suggesting that the TrkH-TrkA complex couples changes in intracellular nucleotides to membrane potential.

TrkA undergoes a tetramer-to-dimer conversion to open TrkH which enables changes in membrane potential.

In many viruses, including rotavirus (RV), the major pathogen of infantile gastroenteritis, capping of viral messenger RNAs is a pivotal step for efficient translation of the viral genome. In RV, VP3 caps the nascent transcripts synthesized from the genomic dsRNA segments by the RV polymerase VP1 within the particle core. Here, from cryo-electron microscopy, x-ray crystallography, and biochemical analyses, we show that VP3 forms a stable tetrameric assembly with each subunit having a modular domain organization, which uniquely integrates five distinct enzymatic steps required for capping the transcripts. In addition to the previously known guanylyl- and methyltransferase activities, we show that VP3 exhibits hitherto unsuspected RNA triphosphatase activity necessary for initiating transcript capping and RNA helicase activity likely required for separating the RNA duplex formed transiently during endogenous transcription. From our studies, we propose a new mechanism for how VP3 inside the virion core caps the nascent transcripts exiting from the polymerase.

2.7 Å cryo-EM structure of rotavirus core protein VP3, a unique capping machine with a helicase activity.

Steroid receptors activate gene transcription by recruiting coactivators to initiate transcription of their target genes. For most nuclear receptors, the ligand-dependent activation function domain-2 (AF-2) is a primary contributor to the nuclear receptor (NR) transcriptional activity. In contrast to other steroid receptors, such as ERα, the activation function of androgen receptor (AR) is largely dependent on its ligand-independent AF-1 located in its N-terminal domain (NTD). It remains unclear why AR utilizes a different AF domain from other receptors despite that NRs share similar domain organizations. Here, we present cryoelectron microscopy (cryo-EM) structures of DNA-bound full-length AR and its complex structure with key coactivators, SRC-3 and p300. AR dimerization follows a unique head-to-head and tail-to-tail manner. Unlike ERα, AR directly contacts a single SRC-3 and p300. The AR NTD is the primary site for coactivator recruitment. The structures provide a basis for understanding assembly of the AR:coactivator complex and its domain contributions for coactivator assembly and transcriptional regulation.

Article	Year
An examination of the electrostatic interactions between the N-terminal tail of the Brome Mosaic Virus coat protein and encapsidated RNAs. Journal of molecular biology\| PubMed ID: 22472420	2012
Structure of the AcrAB-TolC multidrug efflux pump. Nature\| PubMed ID: 24747401
An atomic model of brome mosaic virus using direct electron detection and real-space optimization. Nature communications\| PubMed ID: 25185801
Structure of a biologically active estrogen receptor-coactivator complex on DNA. Molecular cell\| PubMed ID: 25728767
Two polar residues within C-terminal domain of M1 are critical for the formation of influenza A Virions. Cellular microbiology\| PubMed ID: 25939747	2015
Gating machinery of InsP3R channels revealed by electron cryomicroscopy. Nature\| PubMed ID: 26458101
Accurate model annotation of a near-atomic resolution cryo-EM map. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 28270620
An allosteric transport mechanism for the AcrAB-TolC multidrug efflux pump. eLife\| PubMed ID: 28355133
Structural and Functional Impacts of ER Coactivator Sequential Recruitment. Molecular cell\| PubMed ID: 28844863
Purification of AcrAB-TolC Multidrug Efflux Pump for Cryo-EM Analysis. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 29177826
Cryo-EM reveals ligand induced allostery underlying InsPR channel gating. Cell research\| PubMed ID: 30470765
Structures of TRPV2 in distinct conformations provide insight into role of the pore turret. Nature structural & molecular biology\| PubMed ID: 30598551
Structure basis of neutralization by a novel site II/IV antibody against respiratory syncytial virus fusion protein. PloS one\| PubMed ID: 30730999
In situ structure and assembly of the multidrug efflux pump AcrAB-TolC. Nature communications\| PubMed ID: 31201302
Structural basis for the adsorption of a single-stranded RNA bacteriophage. Nature communications\| PubMed ID: 31311931	2019
A complete data processing workflow for cryo-ET and subtomogram averaging. Nature methods\| PubMed ID: 31611690
TrkA undergoes a tetramer-to-dimer conversion to open TrkH which enables changes in membrane potential. Nature communications\| PubMed ID: 31992706
2.7 Å cryo-EM structure of rotavirus core protein VP3, a unique capping machine with a helicase activity. Science advances\| PubMed ID: 32494598
Structural Insights of Transcriptionally Active, Full-Length Androgen Receptor Coactivator Complexes. Molecular cell\| PubMed ID: 32668201

Zhao Wang

Biography

JoVE Journal Publications

Other Publications