automating-tumor-implantation-zebrafish-larvae-for-cancer-research

Automating Tumor Implantation in Zebrafish Larvae for Cancer Research and Medicine

Zebrafish larval injections, including subcutaneous and brain-orthotopic tumor xenografting as well as intravenous drug administration, have become ...

the-causal-effects-plasma-metabolites-on-achilles-tendon-injury

The Causal Effects of Plasma Metabolites on Achilles Tendon Injury: A Mendelian Randomization Study

To investigate the causal effects of plasma metabolites on the progression and recovery of Achilles tendon injury (ATI), Mendelian randomization (MR) was ...

Life Science Methods BV

Ningxia medical university

In this study, the authors compared the efficiency of automated robotic and manual injection methods for the CRISPR-RfxCas13d (CasRx) system for mRNA knockdown and Cas9-mediated DNA targeting in zebrafish embryos. They targeted the no tail () gene as a proof-of-principle, evaluating the induced embryonic phenotypes. Both Cas9 and CasRx systems caused loss of function phenotypes for . Cas9 protein exhibited a higher percentage of severe phenotypes compared with mRNA, while CasRx protein and mRNA showed similar efficiency. Both robotic and manual injections demonstrated comparable phenotype percentages and mortality rates. The findings highlight the potential of RNA-targeting CRISPR effectors for precise gene knockdown and endorse automated microinjection at a speed of 1.0 s per embryo as a high-throughput alternative to manual methods.

Comparing robotic and manual injection methods in zebrafish embryos for high-throughput RNA silencing using CRISPR-RfxCas13d.

The incidence of non-tuberculous mycobacteria (NTM) is increasing globally, often surpassing the incidence of new tuberculosis (TB) cases in developed countries. Most NTM are environmental organisms; however, there are a number of opportunistic and pathogenic species that can cause severe infections in animals and humans. Many NTM are intrinsically resistant to anti-TB therapies and are incredibly difficult to treat, resulting in poor treatment outcomes for these patients. Recent advances in preclinical animal models such as the zebrafish models have led to the discovery of highly active antimicrobial and host-directed therapies (HDTs) targeting NTM infections that can be applied to treat human infections. Here, we summarize recent progress and technological advancements in the discovery and development of antimicrobial drugs and HDTs that have been applied to NTM zebrafish infection models. We highlight the future directions for this increasingly applicable animal model for the discovery of next-generation therapies to treat NTM diseases.

Zebrafish Models for Drug Discovery and Therapeutic Validation against Non-Tuberculous Mycobacteria.

The aim of this study was to identify whether policies in different stages of medical system reform had been effective in decreasing inequalities and increasing the density of health workers in rural areas in China between 1985 and 2011.

Inequality trends of health workforce in different stages of medical system reform (1985-2011) in China.

The change of public service has usually been considered to affect public happiness. However, since the publication of the Easterlin Paradox, the causal relationship between public service and public happiness has been furiously questioned by public affairs researchers. It has been documented through resolving the four causal factors of public happiness within public administration, new public administration, new public management, and governance that public-service-driven public happiness may be attributed to four happiness dimensions: Objective Reality, Subjective Reality, Inter-Subjective Reality, and Virtual Reality. This article reports the results of significance tests of the relationship between public service and public happiness from analyses of large datasets collected from Weibo systems in 31 Chinese provincial governments from 2010 to 2020. The analyses show that the public service change during this period has not yet led to satisfactory improvement in all four happiness dimensions. Finally, we propose strategies for governments to modify public services to enhance public happiness.

Public Service and Public Happiness: Inferences From Big Weibo Datasets for 31 Chinese Provincial Governments.

Two β-Diketiminate cobalt(II) compounds of formula [LCo(μ-Cl)]·2CH (1) and [LCoClPy]·0.5CH·0.5CH (2) (L = [PhC-(PhCN-Dip)], Dip = 2,6-PrCH) have been synthesized and structurally characterized by single crystal X-ray diffraction. Compound 1 exhibits a dinuclear structure, whereas 2 is a mononuclear structure having a pyridine ligand. The tetra-coordinate Co centers in both compounds show distorted tetrahedral geometry configuration. Magnetic measurements reveal that only 2 exhibits field-induced slow relaxation of magnetization with an effective spin-reversal energy barrier of 16.27 K, though large easy-axis magnetic anisotropies for both compounds are unveiled by the analysis of  calculations. Dimeric Co-Co units in 1 indicate intramolecular antiferromagnetic interaction. Compared to individual Co fragment of 1, the first excited quartet state of 2 is strongly destabilized, leading to a smaller unquenched orbital angular momentum and  value. The comprehensive factors above are responsible for the difference in the magnetic behavior between 1 and 2.

Modulation of architectures and magnetic dynamics in pseudotetrahedral cobalt(II) complexes.

A new mononuclear Dy(iii) complex, with the formula [Dy(Hcpt)]·2HO (1), has been successfully prepared  self-assembly between Dy(iii) ions and 2-cyano-'-(1-(pyridin-2-yl)amido)acetyl (Hcpt) ligand. X-ray diffraction study shows that the Dy(iii) ion is nine-coordinated by three Hcpt ligands with a tridentate chelating mode, leading to an approximately monocapped square-antiprismatic ( ) geometry. Magnetic data analysis demonstrates that 1 performs field-induced slow magnetic relaxation with a relaxation barrier of 97.90 K, due to the quantum tunneling effect suppressed upon a static dc field of 2000 Oe. To deeply understand the magnetic behaviors, the relaxation mechanisms and magneto-structure relationship are rationally discussed using  calculations as well.

A mononuclear nine-coordinated Dy(iii) complex exhibiting field-induced single-ion magnetism behaviour.

Over the past decade, cross-sectional studies have established a link between competitive classroom climate and learning motivation. However, the precise predictive direction has remained unclear, and the potential mechanisms underlying the link have yet to be investigated. According to the social comparison theory, competitive classroom climate is positively associated with learning motivation, and upward and downward comparison may play a role in mediating this process.

The Concurrent and Longitudinal Relations Between Competitive Classroom Climate and Learning Motivation Among Chinese Adolescent Students: The Mediating Roles of Social Comparisons.

To compare the effect of three different surgical methods on rabbit Achilles tendon rupture.

Comparison of the Effects of Open Surgery and Minimally Invasive Surgery on the Achilles Tendon Rupture Healing Based on Angiogenesis.

Fast and selective detection of contaminants plays a key role in meeting human health and environmental concerns. Herein, two groups of isostructural lanthanide MOFs, [Ln(Hpta)(oxalic acid)]·HO (1-Eu, 2-Gd) and [Ln(pta)(oxalic acid)(HO)]·2HO (3-Eu, 4-Gd) (Hpta = 2-(4-pyridyl)-terephthalic acid, CO = oxalic acid), were synthesized by solvothermal method. Single crystal X-ray diffraction reveals that 1 and 2 are 3D neutral frameworks, while 3 and 4 consist of 2D layers with parallelogram holes and stack into 3D networks through O-H⋯N and O-H⋯O hydrogen bonding interactions. All complexes remain crystalline and stable below 400 °C, suggesting preeminent thermostability. Noteworthily, only 3 shows excellent chemical stability in water and organic solvent. Therefore, the solid-state fluorescence spectrum was used to characterize 3 which exhibited intense red luminescence. The N active sites in the pore channels of 3 are conducive to displaying a distinct quenching effect for Fe cations in aqueous solutions, CrO anions in DMF and DMA solutions, and nitrofuran antibiotics in the DMF solvent. Overall, 3 is a prospective luminescent sensor for detecting Fe, CrO and nitrofuran antibiotics.

Lanthanide-MOFs as multi-responsive photoluminescence sensor for sensitively detecting Fe, CrO and nitrofuran antibiotics.

Selective neck dissection (SND) involving levels II–IV for N1b papillary thyroid carcinoma (PTC) may compromise spinal accessory nerve (SAN) function. Robotic thyroidectomy via the bilateral axillo-breast approach (BABA RT) offers technical advantages for SAN preservation. We compared SAN functional outcomes following BABA RT versus open thyroid surgery (OTS) with SND for N1b PTC in a prospective cohort (50 patients per group; July 2024 to December 2024) and a 1:1 propensity score–matched (PSM) retrospective cohort (121 per group; December 2020 to June 2024). SAN function was assessed via electromyography (EMG), active shoulder abduction, trapezius muscle strength, and the Shoulder Pain and Disability Index (SPADI). Oncologic outcomes were evaluated. The prospective cohort showed BABA RT outperformed OTS at 3 months postoperatively: greater SPADI reduction (median difference [MD], -5.5 [95% CI, -8.0 to -3.0];  < 0.001), greater shoulder abduction (MD, 9.5° [95% CI, 6.8 to 12.1];  < 0.001), and better EMG scores and trapezius muscle strength ( < 0.001). 76.0% of BABA RT patients achieved shoulder abduction ≥ 151° within 3 months versus 14.0% with OTS ( < 0.001). This advantage persisted at 6 months, with a higher rate of SPADI ≤ 2 (56.0% vs. 28.0%;  = 0.005). A retrospective PSM-matched cohort confirmed the SPADI advantage at 6 months. Both cohorts showed BABA RT retrieved more level IIb nodes with comparable oncologic safety. BABA RT provides superior SAN functional outcomes and higher level IIb nodal yield than OTS, supporting its use in selected patients with N1b PTC.

Superior spinal accessory nerve function after robotic versus open selective neck dissection in papillary thyroid carcinoma: a retrospective and prospective cohort study.

ArticleTotal : 2	Year
Publication title	2025
Publication title	2026

Article	Year
Comparing robotic and manual injection methods in zebrafish embryos for high-throughput RNA silencing using CRISPR-RfxCas13d. BioTechniques\| PubMed ID: 38420933	2024
Zebrafish Models for Drug Discovery and Therapeutic Validation against Non-Tuberculous Mycobacteria. Cold Spring Harbor perspectives in medicine\| PubMed ID: 40147945	2025
Inequality trends of health workforce in different stages of medical system reform (1985-2011) in China. Human resources for health\| PubMed ID: 26645960	2015
Public Service and Public Happiness: Inferences From Big Weibo Datasets for 31 Chinese Provincial Governments. Frontiers in big data\| PubMed ID: 35464123	2022
Modulation of architectures and magnetic dynamics in pseudotetrahedral cobalt(II) complexes. Dalton transactions (Cambridge, England : 2003)\| PubMed ID: 35514278	2022
A mononuclear nine-coordinated Dy(iii) complex exhibiting field-induced single-ion magnetism behaviour. RSC advances\| PubMed ID: 35558827	2022
The Concurrent and Longitudinal Relations Between Competitive Classroom Climate and Learning Motivation Among Chinese Adolescent Students: The Mediating Roles of Social Comparisons. Psychology research and behavior management\| PubMed ID: 35592765	2022
Comparison of the Effects of Open Surgery and Minimally Invasive Surgery on the Achilles Tendon Rupture Healing Based on Angiogenesis. Computational intelligence and neuroscience\| PubMed ID: 36093506	2022
Lanthanide-MOFs as multi-responsive photoluminescence sensor for sensitively detecting Fe, CrO and nitrofuran antibiotics. RSC advances\| PubMed ID: 37671001	2023
Superior spinal accessory nerve function after robotic versus open selective neck dissection in papillary thyroid carcinoma: a retrospective and prospective cohort study. Journal of robotic surgery\| PubMed ID: 41803606	2026

Yi Ding

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