analyses-competing-endogenous-rna-network-rats-with-middle-cerebral

Analyses of the Competing Endogenous RNA Network in Rats with Middle Cerebral Artery Occlusion Based on lncRNA, miRNA, and mRNA Expression

This study aims to investigate the regulatory roles and underlying mechanisms of lncRNAs acting as ceRNAs in ischemic stroke. Based on the ceRNA ...

effect-piribedil-on-gut-microbiota-patients-with-parkinson-s

Effect of Piribedil on the Gut Microbiota of Patients with Parkinson&#39;s Disease

This study aimed to determine whether piribedil therapy alters the gut microbiota of patients with Parkinson&#8217;s disease (PD), providing a theoretical ...

The First Affiliated Hospital of Anhui University of Chinese Medicine

Anhui University of Chinese Medicine

Taohong Siwu Decoction (THSWD) is a traditional Chinese herbal prescription that is effective for ischemic stroke, Whether THSWD regulates the CCL2/CCR2 axis and thus reduces the inflammatory response induced by ischemic stroke is not known. The aim of this study was to elucidate the mechanism of action of THSWD in the treatment of ischemic stroke using bioinformatics combined with  and  experiments.

Exploration of the mechanism of Taohong Siwu Decoction for the treatment of ischemic stroke based on CCL2/CCR2 axis.

Ischemic stroke (IS) is the most prevalent type of cerebrovascular disease. Taohong Siwu Decoction (THSWD) has been demonstrated to have neuroprotective benefits during Cerebral Ischemia-Reperfusion Injury (CIRI). Whether THSWD mitigates CIRI by modulating the circDnajc1/miR-27a-5p/C1qc signaling axis is not known.

Exploring the mechanism of Taohong Siwu Decoction in treating ischemic stroke injury via the circDnajc1/miR-27a-5p/C1qc signaling axis.

To date, our understanding of the relationship between middle cerebral artery occlusion/reperfusion (MCAO/R)-induced ischemia stroke and circular RNAs (circRNAs) remains limited. The objective of this research was to explore the roles and underlying mechanisms of the circDnajc1/miR-27a-5p/C1qc signaling pathway in the development of ischemia stroke. For in vitro experiments, an oxygen-glucose deprivation/reperfusion (OGD/R) cell model was established following neuron-microglia co-culture. circDnajc1 siRNA and pcDNA3.1-circDnajc1 overexpression vectors were constructed and transfected into primary microglia. For in vivo experiments, an MCAO/R rat model was established. The molecular mechanisms were investigated using flow cytometry, RT-qPCR, immunofluorescence, RNA immunoprecipitation, and luciferase reporter gene assays. In vitro experiments showed that circDnajc1-induced cellular activation following microglia OGD/R injury, promoted the release of inflammatory factors, downregulated miR-27a-5p, upregulated C1qc, C3, and C5ar, and promotes neuronal apoptosis after microglia OGD/R injury. Interference with circDnajc1 reversed these effects. In vivo experiments, circDnajc1 knockdown exerted a protective effect on neurons in MCAO/R rats and inhibited microglial activation and the release of inflammatory factors. Consistent with the in vitro experiments, circDnajc1 regulated on the expression of downstream target genes of miR-27a-5p, C1qc, C3, and C5ar. CircDnajc1 plays a critical role in ischemia stroke and may influence nervous system homeostasis by regulating the circDnajc1/miR-27a-5p/C1qc signaling axis, which promotes microglia activation and regulates inflammatory factor release and neuronal apoptosis.

Exploring the Role of Microglia Activation in Ischemia Stroke Based on the circDnajc1/miR-27a-5p/C1qc Signaling Axis.

Taohong Siwu Decoction (THSWD), a classic prescription, is commonly used to treat ischemic stroke. However, the effects of THSWD on endothelial cells (ECs) in cerebral ischemia-reperfusion injury (IRI) are unclear.

ArticleTotal : 2	Year
Publication title Cited by 1	2025
Publication title	2026

Article	Year
Exploration of the mechanism of Taohong Siwu Decoction for the treatment of ischemic stroke based on CCL2/CCR2 axis. Frontiers in pharmacology\| PubMed ID: 39268469	2024
Exploring the mechanism of Taohong Siwu Decoction in treating ischemic stroke injury via the circDnajc1/miR-27a-5p/C1qc signaling axis. Phytomedicine : international journal of phytotherapy and phytopharmacology\| PubMed ID: 39626448	2025
Exploring the Role of Microglia Activation in Ischemia Stroke Based on the circDnajc1/miR-27a-5p/C1qc Signaling Axis. Molecular neurobiology\| PubMed ID: 40483386	2025
Regulation of NR4A1 by Taohong Siwu decoction inhibits endothelial cell apoptosis in cerebral Ischemia-Reperfusion injury. Journal of ethnopharmacology\| PubMed ID: 40653039	2025

Shuangping Li

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