analyses-competing-endogenous-rna-network-rats-with-middle-cerebral

Analyses of the Competing Endogenous RNA Network in Rats with Middle Cerebral Artery Occlusion Based on lncRNA, miRNA, and mRNA Expression

This study aims to investigate the regulatory roles and underlying mechanisms of lncRNAs acting as ceRNAs in ischemic stroke. Based on the ceRNA ...

Anhui University of Chinese Medicine

Xin'an Medical Research Institute

Ministry of Education

Anhui Province Key Laboratory of Chinese Medicinal Formula

You-Gui Yin (YGY) is a traditional Chinese medicine (TCM) decoction composed of eight Chinese herbs. The interaction between TCM and Western medicine has attracted much attention nowadays. It is therefore necessary to study the clinical application of YGY in combination with Western medicine from the perspective of metabolic enzymes. This study aims to investigate the effect of YGY on the activities of seven CYP450 isozymes (CYP1A2, CYP2B6, CYP2C8, CYP2C9, CYP2C19, CYP2D6, and CYP3A4) in rats. Twenty-four Sprague-Dawley (SD) rats were randomly divided into four groups: high, middle, and low-dose YGY-treated groups and the control group. They were given 13.78, 20.67, and 31 g/kg/d YGY decoction by oral administration and normal saline (10 mL/kg), respectively, for 14 days. Half an hour after the last administration, a mixed probe substrate (1 mg/kg) was administered by tail vein injection. Then, blood was taken from the venous plexus at different time points. The protein expression level of the CYP450 enzymes in the control and treatment groups was determined by western blot. The effect of YGY on the activity of CYP isoenzymes was studied by comparing the plasma pharmacokinetics between the control and treatment groups. Compared with the control group, YGY at a high (31 g/kg) dosage could decrease AUC, AUC and   of diclofenac, omeprazole, and midazolam by at least 35.4%, while increase CL by at least 88.9%; this revealed that YGY could induce CYP2C9, CYP2C19, and CYP3A4. The results show that when we use You-Gui Yin decoction in combination with other drugs, especially drugs metabolized by CYP2C9, CYP2C19, and CYP3A4 enzymes, the interaction between drugs needs special attention.

Effect of You-Gui Yin on the Activities of Seven Cytochrome P450 Isozymes in Rats.

Zuo-Gui Yin Decoction (ZGYD), a traditional Chinese prescription, is mainly used in various kinds of andrology and gynecology diseases. However, the study on the interaction of ZGYD and drugs has not been reported. Therefore, evaluating the interaction between ZGYD and metabolic enzymes is helpful to guide rational drug use.

Evaluation of Zuo-Gui Yin Decoction Effects on Six CYP450 Enzymes in Rats Using a Cocktail Method by UPLC-MS/MS.

To date, our understanding of the relationship between middle cerebral artery occlusion/reperfusion (MCAO/R)-induced ischemia stroke and circular RNAs (circRNAs) remains limited. The objective of this research was to explore the roles and underlying mechanisms of the circDnajc1/miR-27a-5p/C1qc signaling pathway in the development of ischemia stroke. For in vitro experiments, an oxygen-glucose deprivation/reperfusion (OGD/R) cell model was established following neuron-microglia co-culture. circDnajc1 siRNA and pcDNA3.1-circDnajc1 overexpression vectors were constructed and transfected into primary microglia. For in vivo experiments, an MCAO/R rat model was established. The molecular mechanisms were investigated using flow cytometry, RT-qPCR, immunofluorescence, RNA immunoprecipitation, and luciferase reporter gene assays. In vitro experiments showed that circDnajc1-induced cellular activation following microglia OGD/R injury, promoted the release of inflammatory factors, downregulated miR-27a-5p, upregulated C1qc, C3, and C5ar, and promotes neuronal apoptosis after microglia OGD/R injury. Interference with circDnajc1 reversed these effects. In vivo experiments, circDnajc1 knockdown exerted a protective effect on neurons in MCAO/R rats and inhibited microglial activation and the release of inflammatory factors. Consistent with the in vitro experiments, circDnajc1 regulated on the expression of downstream target genes of miR-27a-5p, C1qc, C3, and C5ar. CircDnajc1 plays a critical role in ischemia stroke and may influence nervous system homeostasis by regulating the circDnajc1/miR-27a-5p/C1qc signaling axis, which promotes microglia activation and regulates inflammatory factor release and neuronal apoptosis.

Article	Year
Effect of You-Gui Yin on the Activities of Seven Cytochrome P450 Isozymes in Rats. Evidence-based complementary and alternative medicine : eCAM\| PubMed ID: 32508959	2020
Evaluation of Zuo-Gui Yin Decoction Effects on Six CYP450 Enzymes in Rats Using a Cocktail Method by UPLC-MS/MS. BioMed research international\| PubMed ID: 36060135	2022
Exploring the Role of Microglia Activation in Ischemia Stroke Based on the circDnajc1/miR-27a-5p/C1qc Signaling Axis. Molecular neurobiology\| PubMed ID: 40483386	2025

Shizhong Hong

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