developmental-toxicity-assay-based-on-real-time-monitoring-fibroblast

Developmental Toxicity Assay Based on Real-Time Monitoring of Fibroblast Growth Factor Signal Disruption in Human Induced Pluripotent Stem Cells

Developmental toxicity (Devtox) is typically evaluated in animal models. However, there are challenges in ensuring accuracy, such as false negatives due ...

Yokohama National University

National Institute of Health Sciences

The vascular and neuronal networks are structurally complex and highly branched. These networks play an important role in supplying oxygen and sending signals to the tissues and organs in the body. This study proposes procedures to prepare in vitro neurovascular models on glass substrates and collagen gels for a better understanding of neurovascular interactions. Human umbilical vein endothelial cells (HUVECs) elongated the neurites of neuronal model cells (PC12 cells) on a glass substrate. In collagen gel cultures, the timing of supplementing nerve growth factor (NGF) was crucial for the formation of vascular and neural networks. Thus, neurite outgrowth was more effectively promoted by initially culturing the two cell types in the medium for vascular endothelial cells for 5 days to induce vascular organization and then adding NGF, rather than culturing the cells in the presence of NGF from the beginning. This simple sequential method may be useful for preparing 3-dimensional neurovascular models.

Koki Murayama

Biography

JoVE Journal Publications

Other Publications