measuring-intestinal-barrier-permeability-detecting-flux-fluorescent

Measuring Intestinal Barrier Permeability by Detecting Flux of Fluorescent Tracers Across Human Colonoid Monolayers

The intestinal barrier is a critical site of regulation between luminal antigens and the host immune system, and its dysregulation is implicated in ...

Children's Hospital Colorado

University of Colorado

Inflammatory bowel disease is associated with intestinal energetic derangements, including reduced creatine kinase (CK) expression. CK is critical to cellular energetics, catalyzing bidirectional transfer of high-energy phosphate between creatine and ATP, and phosphocreatine (PCr) and ADP. However, the impact of CK loss on intestinal epithelial cells (IECs) remains unclear. We aimed to characterize energetic and functional consequences of CK deficiency in IECs and identify a treatment to circumvent CK loss.

Ciara Schaepe

Biography

JoVE Journal Publications

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