collection-human-follicular-fluid-follicle-somatic-cells-immature

Collection of Human Follicular Fluid, Follicle Somatic Cells, and Immature Oocytes from Individuals Undergoing<em> In Vitro </em>Fertilization

Human ovary follicular contents, including follicular fluid (FF), oocytes, cumulus cells, and somatic cells, offer a powerful yet underutilized ...

Northwestern University

To evaluate the relationship between maternal body mass index (BMI) and embryonic aneuploidy of maternal origin.

Maternal body mass index is not associated with increased rates of maternal embryonic aneuploidy.

To determine if there is a relationship between paternal factors and embryonic aneuploidy of paternal origin using preimplantation genetic testing for aneuploidy (PGT-A).

Relationship between paternal factors and embryonic aneuploidy of paternal origin.

Specific serum beta human chorionic gonadotropin (β-hCG) parameters that can predict live birth after an embryo transfer have yet to be defined.

Early β-hCG levels predict live birth after single embryo transfer.

To assess childbearing intentions, concerns about future fertility, knowledge about the age-related decline in fertility, and interest in receiving fertility education among undergraduate students.

Undergraduate students have low fertility knowledge and high anxiety regarding future fertility: An opportunity for education.

To describe the serum anti-Müllerian hormone (AMH) concentrations in a large, well-phenotyped cohort of women with polycystic ovary syndrome (PCOS) and evaluate whether AMH predicts successful ovulation induction in women treated with clomiphene and metformin.

Antimüllerian hormone level predicts ovulation in women with polycystic ovary syndrome treated with clomiphene and metformin.

Adverse pregnancy outcomes (APOs), including hypertensive disorders of pregnancy (HDP), low birthweight (LBW), and preterm birth (PTB), along with peripartum cardiomyopathy (PPCM) are associated with short- and long-term maternal and fetal cardiovascular risks. This review focuses on the genetic contributions to the risk of APOs and PPCM.

Genetic contributions to risk of adverse pregnancy outcomes.

This study aimed to determine the association between changes in age distribution and maternal mortality rates (MMR) in a subset of the United States between 2014 and 2021.

Changes in Age Distribution and Maternal Mortality in a Subset of the U.S., 2014-2021.

The ovary is one of the first organs to show overt signs of aging in the human body, and ovarian aging is associated with a loss of gamete quality and quantity. The age-dependent decline in ovarian function contributes to infertility and an altered endocrine milieu, which has ramifications for overall health. The aging ovarian microenvironment becomes fibro-inflammatory and stiff with age, and this has implications for ovarian physiology and pathology, including follicle growth, gamete quality, ovulation dynamics, and ovarian cancer. Thus, developing a non-invasive tool to measure and monitor the stiffness of the human ovary would represent a major advance for female reproductive health and longevity. Shear wave elastography is a quantitative ultrasound imaging method for evaluation of soft tissue stiffness. Shear wave elastography has been used clinically in assessment of liver fibrosis and characterization of tendinopathies and various neoplasms in thyroid, breast, prostate, and lymph nodes as a non-invasive diagnostic and prognostic tool. In this study, we review the underlying principles of shear wave elastography and its current clinical uses outside the reproductive tract as well as its successful application of shear wave elastography to reproductive tissues, including the uterus and cervix. We also describe an emerging use of this technology in evaluation of human ovarian stiffness via transvaginal ultrasound. Establishing ovarian stiffness as a clinical biomarker of ovarian aging may have implications for predicting the ovarian reserve and outcomes of Assisted Reproductive Technologies as well as for the assessment of the efficacy of emerging therapeutics to extend reproductive longevity. This parameter may also have broad relevance in other conditions where ovarian stiffness and fibrosis may be implicated, such as polycystic ovarian syndrome, late off target effects of chemotherapy and radiation, premature ovarian insufficiency, conditions of differences of sexual development, and ovarian cancer. Summary sentence:  Shear Wave Elastography is a non-invasive technique to study human tissue stiffness, and here we review its clinical applications and implications for reproductive health and disease.

Article	Year
Maternal body mass index is not associated with increased rates of maternal embryonic aneuploidy. Fertility and sterility\| PubMed ID: 35105446	2022
Relationship between paternal factors and embryonic aneuploidy of paternal origin. Fertility and sterility\| PubMed ID: 35637025	2022
Early β-hCG levels predict live birth after single embryo transfer. Journal of assisted reproduction and genetics\| PubMed ID: 36074224	2022
Undergraduate students have low fertility knowledge and high anxiety regarding future fertility: An opportunity for education. Heliyon\| PubMed ID: 36967893	2023
Antimüllerian hormone level predicts ovulation in women with polycystic ovary syndrome treated with clomiphene and metformin. Fertility and sterility\| PubMed ID: 38154770	2024
Genetic contributions to risk of adverse pregnancy outcomes. Current cardiovascular risk reports\| PubMed ID: 38186860	2023
Changes in Age Distribution and Maternal Mortality in a Subset of the U.S., 2014-2021. American journal of preventive medicine\| PubMed ID: 38506785	2024
Shear wave elastography to assess stiffness of the human ovary and other reproductive tissues across the reproductive lifespan in health and disease†. Biology of reproduction\| PubMed ID: 38609185	2024
The ethics of egg sharing. Fertility and sterility\| PubMed ID: 39520425	2024
It is time to rethink coordination of fresh oocyte retrievals with microscopic testicular sperm extraction. Fertility and sterility\| PubMed ID: 39732276	2025
Ovarian aging: a missing diagnosis in reproductive medicine. Nature medicine\| PubMed ID: 40481207	2025

Lydia Hughes

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